Mouse Monoclonal HADHB antibody. Suitable for ICC, IP, Flow Cyt, WB and reacts with Human samples. Cited in 2 publications.
Images
![Western blot - Anti-HADHB antibody [5C3AB7] (AB110301), expandable thumbnail](https://content.abcam.com/products/images/hadhb-antibody-5c3ab7-ab110301--western-blot-img21518.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-HADHB antibody [5C3AB7] (AB110301)")
![Immunocytochemistry - Anti-HADHB antibody [5C3AB7] (AB110301), expandable thumbnail](https://content.abcam.com/products/images/hadhb-antibody-5c3ab7-ab110301--immunocytochemistry-img127372.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry - Anti-HADHB antibody [5C3AB7] (AB110301)")
Publications
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- Frontiers in oncology 11:7371272021Multi-Omics Analysis of Fatty Acid Metabolism in Thyroid Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinghui Lu,Yankun Zhang,Min Sun,Changyuan Ding,Lei Zhang,Youzi Kong,Meng Cai,Paolo Miccoli,Chunhong Ma,Xuetian YuePubMed 34976793
- Molecular cell 69:480-492.e72018Nuclear Receptor Nur77 Facilitates Melanoma Cell Survival under Metabolic Stress by Protecting Fatty Acid Oxidation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao-Xue Li et. al.PubMed 29395065
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline- Form
Liquid
- Clonality
Monoclonal
Reactivity data
Select an application| ICC | IP | Flow Cyt | WB | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.5 µg/mL | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
Mitochondrial trifunctional enzyme catalyzes the last three of the four reactions of the mitochondrial beta-oxidation pathway (PubMed:8135828, PubMed:29915090, PubMed:30850536). The mitochondrial beta-oxidation pathway is the major energy-producing process in tissues and is performed through four consecutive reactions breaking down fatty acids into acetyl-CoA (PubMed:29915090). Among the enzymes involved in this pathway, the trifunctional enzyme exhibits specificity for long-chain fatty acids (PubMed:30850536). Mitochondrial trifunctional enzyme is a heterotetrameric complex composed of two proteins, the trifunctional enzyme subunit alpha/HADHA carries the 2,3-enoyl-CoA hydratase and the 3-hydroxyacyl-CoA dehydrogenase activities, while the trifunctional enzyme subunit beta/HADHB described here bears the 3-ketoacyl-CoA thiolase activity (PubMed:8135828, PubMed:29915090, PubMed:30850536).
Alternative names
TP-beta, MSTP029, HADHB
Recommended products
Mouse Monoclonal HADHB antibody. Suitable for ICC, IP, Flow Cyt, WB and reacts with Human samples. Cited in 2 publications.
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline- Form
Liquid
- Clonality
Monoclonal
- Clone number
5C3AB7
- Purification technique
Affinity purification Immunogen
- Light chain type
kappa
- Concentration
- Purification notes
ab110301 was produced in vitro using hybridomas grown in serum-free medium, and then purified by affinity purification.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Product was previously marketed under the MitoSciences sub-brand.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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5 product images
![Western blot - Anti-HADHB antibody [5C3AB7] (ab110301), expandable thumbnail](https://content.abcam.com/products/images/hadhb-antibody-5c3ab7-ab110301--western-blot-img21518.jpg "Western blot - Anti-HADHB antibody [5C3AB7] (ab110301)")
Western blot - Anti-HADHB antibody [5C3AB7] (ab110301)
All lanes: Western blot - Anti-HADHB antibody [5C3AB7] (ab110301) at 0.5 µg/mL
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 51 kDa
![Immunocytochemistry - Anti-HADHB antibody [5C3AB7] (ab110301), expandable thumbnail](https://content.abcam.com/products/images/hadhb-antibody-5c3ab7-ab110301--immunocytochemistry-img127372.jpg "Immunocytochemistry - Anti-HADHB antibody [5C3AB7] (ab110301)")
Immunocytochemistry - Anti-HADHB antibody [5C3AB7] (ab110301)
Immunocytochemistry analysis using ab110301 at 1μg/ml staining HADHB in HDFn cells (paraformaldehyde fixed and Triton X-100 permeabilized).
The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution. DAPI was used to stain the cell nuclei (blue).![Flow Cytometry - Anti-HADHB antibody [5C3AB7] (ab110301), expandable thumbnail](https://content.abcam.com/products/images/hadhb-antibody-5c3ab7-ab110301--flow-cytometry-img60997.gif "Flow Cytometry - Anti-HADHB antibody [5C3AB7] (ab110301)")
Flow Cytometry - Anti-HADHB antibody [5C3AB7] (ab110301)
Flow cytometric analysis using ab110301 at 1μg/ml staining HADHB in HeLa cells (blue). Isotype control antibody (red).
![Western blot - Anti-HADHB antibody [5C3AB7] (ab110301), expandable thumbnail](https://content.abcam.com/products/images/hadhb-antibody-5c3ab7-ab110301--western-blot-img97047.gif "Western blot - Anti-HADHB antibody [5C3AB7] (ab110301)")
Western blot - Anti-HADHB antibody [5C3AB7] (ab110301)
As a Western blot antibody, ab110301 works with HepG2 (human) cells.
All lanes: Western blot - Anti-HADHB antibody [5C3AB7] (ab110301) at 0.5 µg/mL
All lanes: HepG2 cell lysate
Predicted band size: 51 kDa
![Immunoprecipitation - Anti-HADHB antibody [5C3AB7] (ab110301), expandable thumbnail](https://content.abcam.com/products/images/hadhb-antibody-5c3ab7-ab110301--immunoprecipitation-img4013.gif "Immunoprecipitation - Anti-HADHB antibody [5C3AB7] (ab110301)")
Immunoprecipitation - Anti-HADHB antibody [5C3AB7] (ab110301)
As an IP antibody, MS733 works with human samples.
All lanes: Immunoprecipitation - Anti-HADHB antibody [5C3AB7] (ab110301)
Predicted band size: 51 kDa
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