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AB110301

Anti-HADHB antibody [5C3AB7]

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(1 Review)

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(2 Publications)

Mouse Monoclonal HADHB antibody. Suitable for ICC, IP, Flow Cyt, WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

MSTP029, HADHB, TP-beta

5 Images
Flow Cytometry - Anti-HADHB antibody [5C3AB7] (AB110301)
  • Flow Cyt

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Flow Cytometry - Anti-HADHB antibody [5C3AB7] (AB110301)

Flow cytometric analysis using ab110301 at 1μg/ml staining HADHB in HeLa cells (blue). Isotype control antibody (red).

Immunocytochemistry - Anti-HADHB antibody [5C3AB7] (AB110301)
  • ICC

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Immunocytochemistry - Anti-HADHB antibody [5C3AB7] (AB110301)

Immunocytochemistry analysis using ab110301 at 1μg/ml staining HADHB in HDFn cells (paraformaldehyde fixed and Triton X-100 permeabilized).
The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution. DAPI was used to stain the cell nuclei (blue).

Immunoprecipitation - Anti-HADHB antibody [5C3AB7] (AB110301)
  • IP

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Immunoprecipitation - Anti-HADHB antibody [5C3AB7] (AB110301)

As an IP antibody, MS733 works with human samples.

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Immunoprecipitation - Anti-HADHB antibody [5C3AB7] (ab110301)

Predicted band size: 51 kDa

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Western blot - Anti-HADHB antibody [5C3AB7] (AB110301)
  • WB

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Western blot - Anti-HADHB antibody [5C3AB7] (AB110301)

As a Western blot antibody, ab110301 works with HepG2 (human) cells.

All lanes:

Western blot - Anti-HADHB antibody [5C3AB7] (ab110301) at 0.5 µg/mL

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HepG2 cell lysate

Predicted band size: 51 kDa

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Western blot - Anti-HADHB antibody [5C3AB7] (AB110301)
  • WB

Lab

Western blot - Anti-HADHB antibody [5C3AB7] (AB110301)

All lanes:

Western blot - Anti-HADHB antibody [5C3AB7] (ab110301) at 0.5 µg/mL

Predicted band size: 51 kDa

true

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

5C3AB7

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

ICC, IP, Flow Cyt, WB

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1 µg/mL", "ICC-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg/mL", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5 µg/mL", "WB-species-notes": "<p></p>" } } }
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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab110301 was produced in vitro using hybridomas grown in serum-free medium, and then purified by affinity purification.
Storage buffer
pH: 7.5 Preservative: 0.02% Sodium azide Constituents: HEPES buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein HADHB also called the beta subunit of the Mitochondrial Trifunctional Protein is a part of the enzyme system that breaks down fatty acids. It has an approximate molecular mass of 51 kDa. This protein performs mechanical functions that include enoyl-CoA hydratase hydroxyacyl-CoA dehydrogenase and thiolase activities which facilitate the conversion of fatty acids into acetyl-CoA units. HADHB is expressed in tissues with high energy demands especially in the liver heart and skeletal muscle where it plays an essential role in energy production.
Biological function summary

The HADHB protein forms a complex with HADHA creating the Mitochondrial Trifunctional Protein. This complex is involved in the inner mitochondrial membrane's beta-oxidation of long-chain fatty acids. Long-chain fatty acids serve as a vital energy source and the HADHB-HADHA complex makes their metabolism more efficient. This process provides ATP essential for maintaining cellular energy homeostasis especially during fasting and intense exercise.

Pathways

HADHB participates in the fatty acid beta-oxidation pathway a critical component of lipid metabolism. Through this pathway HADHB interacts with proteins like ACADVL and ACADM which catalyze different stages of fatty acid breakdown. Beta-oxidation plays an important role in producing acetyl-CoA subsequently entering the citric acid cycle linking HADHB to energy metabolism. These interactions emphasize the importance of HADHB in maintaining metabolic balance within cells.

HADHB mutations can lead to mitochondrial trifunctional protein deficiency which results in metabolic disorders like fatty acid oxidation disorders and peripheral neuropathy. These conditions impair the body's ability to break down fatty acids affecting energy homeostasis. Disorders related to HADHB may also involve proteins such as CPT1A which transfers fatty acids into mitochondria highlighting the interconnectedness of these metabolic pathways. Understanding HADHB's role in these diseases enhances the potential for targeted therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Mitochondrial trifunctional enzyme catalyzes the last three of the four reactions of the mitochondrial beta-oxidation pathway (PubMed : 29915090, PubMed : 30850536, PubMed : 8135828). The mitochondrial beta-oxidation pathway is the major energy-producing process in tissues and is performed through four consecutive reactions breaking down fatty acids into acetyl-CoA (PubMed : 29915090). Among the enzymes involved in this pathway, the trifunctional enzyme exhibits specificity for long-chain fatty acids (PubMed : 30850536). Mitochondrial trifunctional enzyme is a heterotetrameric complex composed of two proteins, the trifunctional enzyme subunit alpha/HADHA carries the 2,3-enoyl-CoA hydratase and the 3-hydroxyacyl-CoA dehydrogenase activities, while the trifunctional enzyme subunit beta/HADHB described here bears the 3-ketoacyl-CoA thiolase activity (PubMed : 29915090, PubMed : 30850536, PubMed : 8135828).
See full target information HADHB
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 11:737127 PubMed34976793

2021

Multi-Omics Analysis of Fatty Acid Metabolism in Thyroid Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jinghui Lu,Yankun Zhang,Min Sun,Changyuan Ding,Lei Zhang,Youzi Kong,Meng Cai,Paolo Miccoli,Chunhong Ma,Xuetian Yue

Molecular cell 69:480-492.e7 PubMed29395065

2018

Nuclear Receptor Nur77 Facilitates Melanoma Cell Survival under Metabolic Stress by Protecting Fatty Acid Oxidation.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Xue Li,Zhi-Jing Wang,Yu Zheng,Yun-Feng Guan,Peng-Bo Yang,Xiang Chen,Cong Peng,Jian-Ping He,Yuan-Li Ai,Sheng-Fu Wu,Kun-Yi Chien,Qiao Wu,Hang-Zi Chen
View all publications
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Product promise

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