Rabbit Polyclonal HADHB antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Trifunctional enzyme subunit beta, mitochondrial.
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- Frontiers in oncology 12:10698752022Hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit beta gene as a tumour suppressor in stomach adenocarcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYun Li,Jian-Bo Xiong,Zhi-Gang Jie,Hui XiongPubMed 36518312
- Human molecular genetics 31:3144-31602022Mice lacking MBNL1 and MBNL2 exhibit sudden cardiac death and molecular signatures recapitulating myotonic dystrophy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKuang-Yung Lee,Carol Seah,Ching Li,Yu-Fu Chen,Chwen-Yu Chen,Ching-I Wu,Po-Cheng Liao,Yu-Chiau Shyu,Hailey R Olafson,Kendra K McKee,Eric T Wang,Chi-Hsiao Yeh,Chao-Hung WangPubMed 35567413
- Cell death & disease 12:8242021Cyclic AMP-binding protein Epac1 acts as a metabolic sensor to promote cardiomyocyte lipotoxicity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarion Laudette,Yannis Sainte-Marie,Grégoire Cousin,Dorian Bergonnier,Ismahane Belhabib,Stéphanie Brun,Karina Formoso,Loubna Laib,Florence Tortosa,Camille Bergoglio,Bertrand Marcheix,Jan Borén,Olivier Lairez,Jérémy Fauconnier,Alexandre Lucas,Jeanne Mialet-Perez,Cédric Moro,Frank Lezoualc'hPubMed 34471096
- International journal of molecular sciences 21:2020Pyrroloquinoline Quinone Modifies Lipid Profile, but Not Insulin Sensitivity, of Palmitic Acid-Treated L6 Myotubes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElżbieta Supruniuk et. al.PubMed 33171690
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human Trifunctional enzyme subunit beta, mitochondrial. The exact immunogen used to generate this antibody is proprietary information. Database link P55084
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
| Rat | Expected | Tested | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
Species Mouse | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
Species Rat | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10.00000 - 1/100.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Mitochondrial trifunctional enzyme catalyzes the last three of the four reactions of the mitochondrial beta-oxidation pathway (PubMed:8135828, PubMed:29915090, PubMed:30850536). The mitochondrial beta-oxidation pathway is the major energy-producing process in tissues and is performed through four consecutive reactions breaking down fatty acids into acetyl-CoA (PubMed:29915090). Among the enzymes involved in this pathway, the trifunctional enzyme exhibits specificity for long-chain fatty acids (PubMed:30850536). Mitochondrial trifunctional enzyme is a heterotetrameric complex composed of two proteins, the trifunctional enzyme subunit alpha/HADHA carries the 2,3-enoyl-CoA hydratase and the 3-hydroxyacyl-CoA dehydrogenase activities, while the trifunctional enzyme subunit beta/HADHB described here bears the 3-ketoacyl-CoA thiolase activity (PubMed:8135828, PubMed:29915090, PubMed:30850536).
Alternative names
TP-beta, MSTP029, HADHB
Recommended products
Rabbit Polyclonal HADHB antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Trifunctional enzyme subunit beta, mitochondrial.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human Trifunctional enzyme subunit beta, mitochondrial. The exact immunogen used to generate this antibody is proprietary information. Database link P55084
- Purification technique
Affinity purification
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein HADHB also called the beta subunit of the Mitochondrial Trifunctional Protein is a part of the enzyme system that breaks down fatty acids. It has an approximate molecular mass of 51 kDa. This protein performs mechanical functions that include enoyl-CoA hydratase hydroxyacyl-CoA dehydrogenase and thiolase activities which facilitate the conversion of fatty acids into acetyl-CoA units. HADHB is expressed in tissues with high energy demands especially in the liver heart and skeletal muscle where it plays an essential role in energy production.
- Biological function summary
The HADHB protein forms a complex with HADHA creating the Mitochondrial Trifunctional Protein. This complex is involved in the inner mitochondrial membrane's beta-oxidation of long-chain fatty acids. Long-chain fatty acids serve as a vital energy source and the HADHB-HADHA complex makes their metabolism more efficient. This process provides ATP essential for maintaining cellular energy homeostasis especially during fasting and intense exercise.
- Pathways
HADHB participates in the fatty acid beta-oxidation pathway a critical component of lipid metabolism. Through this pathway HADHB interacts with proteins like ACADVL and ACADM which catalyze different stages of fatty acid breakdown. Beta-oxidation plays an important role in producing acetyl-CoA subsequently entering the citric acid cycle linking HADHB to energy metabolism. These interactions emphasize the importance of HADHB in maintaining metabolic balance within cells.
- Associated diseases and disorders
HADHB mutations can lead to mitochondrial trifunctional protein deficiency which results in metabolic disorders like fatty acid oxidation disorders and peripheral neuropathy. These conditions impair the body's ability to break down fatty acids affecting energy homeostasis. Disorders related to HADHB may also involve proteins such as CPT1A which transfers fatty acids into mitochondria highlighting the interconnectedness of these metabolic pathways. Understanding HADHB's role in these diseases enhances the potential for targeted therapeutic strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
Western blot - Anti-HADHB antibody (ab230667)
All lanes: Western blot - Anti-HADHB antibody (ab230667) at 1/500 dilution
Lane 1: BT-474 (Human ductal breast epithelial tumor cell line) cell extracts
Lane 2: SW480 (Human colorectal adenocarcinoma cell line) cell extracts
Lane 3: HepG2 (Human liver hepatocellular carcinoma cell line) cell extracts
Lane 4: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell extracts
Lane 5: Jurkat (Human T cell leukemia cell line from peripheral blood) cell extracts
Lane 6: Mouse liver extracts
Lane 7: Mouse kidney extracts
Lane 8: Mouse heart extracts
Lane 9: Rat liver extracts
Developed using the ECL technique.
Predicted band size: 51 kDa
Immunocytochemistry/ Immunofluorescence - Anti-HADHB antibody (ab230667)
A549 (Human lung carcinoma cell line) cells stained for HADHB using ab230667 at a dilution of 1/10 in ICC/IF.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HADHB antibody (ab230667)
Formalin-fixed, paraffin-embedded human liver tissue stained for HADHB with ab230667 at a 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HADHB antibody (ab230667)
Formalin-fixed, paraffin-embedded human heart tissue stained for HADHB with ab230667 at a 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HADHB antibody (ab230667)
Formalin-fixed, paraffin-embedded human pancreas tissue stained for HADHB with ab230667 at a 1/50 dilution in immunohistochemical analysis.
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