AB230667

Anti-HADHB antibody

Name: Anti-HADHB antibody (ab230667) | Abcam
Brand: Abcam Limited
SKU: AB230667
Price: 585 USD
Availability: InStock

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(5 Publications)

Rabbit Polyclonal HADHB antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HADHB.

View Alternative Names

MSTP029, HADHB, TP-beta

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HADHB antibody (AB230667)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HADHB antibody (AB230667)

Formalin-fixed, paraffin-embedded human heart tissue stained for HADHB with ab230667 at a 1/50 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-HADHB antibody (AB230667)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-HADHB antibody (AB230667)

A549 (Human lung carcinoma cell line) cells stained for HADHB using ab230667 at a dilution of 1/10 in ICC/IF.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HADHB antibody (AB230667)

Formalin-fixed, paraffin-embedded human liver tissue stained for HADHB with ab230667 at a 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HADHB antibody (AB230667)

Formalin-fixed, paraffin-embedded human pancreas tissue stained for HADHB with ab230667 at a 1/50 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-HADHB antibody (AB230667)

All lanes:

Western blot - Anti-HADHB antibody (ab230667) at 1/500 dilution

Lane 1:

BT-474 (Human ductal breast epithelial tumor cell line) cell extracts

Lane 2:

SW480 (Human colorectal adenocarcinoma cell line) cell extracts

Lane 3:

HepG2 (Human liver hepatocellular carcinoma cell line) cell extracts

Lane 4:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell extracts

Lane 5:

Jurkat (Human T cell leukemia cell line from peripheral blood) cell extracts

Lane 6:

Mouse liver extracts

Lane 7:

Mouse kidney extracts

Lane 8:

Mouse heart extracts

Lane 9:

Rat liver extracts

Predicted band size: 51 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human HADHB. The exact immunogen used to generate this antibody is proprietary information.

P55084

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/10 - 1/100", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein HADHB also called the beta subunit of the Mitochondrial Trifunctional Protein is a part of the enzyme system that breaks down fatty acids. It has an approximate molecular mass of 51 kDa. This protein performs mechanical functions that include enoyl-CoA hydratase hydroxyacyl-CoA dehydrogenase and thiolase activities which facilitate the conversion of fatty acids into acetyl-CoA units. HADHB is expressed in tissues with high energy demands especially in the liver heart and skeletal muscle where it plays an essential role in energy production.

Biological function summary

The HADHB protein forms a complex with HADHA creating the Mitochondrial Trifunctional Protein. This complex is involved in the inner mitochondrial membrane's beta-oxidation of long-chain fatty acids. Long-chain fatty acids serve as a vital energy source and the HADHB-HADHA complex makes their metabolism more efficient. This process provides ATP essential for maintaining cellular energy homeostasis especially during fasting and intense exercise.

Pathways

HADHB participates in the fatty acid beta-oxidation pathway a critical component of lipid metabolism. Through this pathway HADHB interacts with proteins like ACADVL and ACADM which catalyze different stages of fatty acid breakdown. Beta-oxidation plays an important role in producing acetyl-CoA subsequently entering the citric acid cycle linking HADHB to energy metabolism. These interactions emphasize the importance of HADHB in maintaining metabolic balance within cells.

HADHB mutations can lead to mitochondrial trifunctional protein deficiency which results in metabolic disorders like fatty acid oxidation disorders and peripheral neuropathy. These conditions impair the body's ability to break down fatty acids affecting energy homeostasis. Disorders related to HADHB may also involve proteins such as CPT1A which transfers fatty acids into mitochondria highlighting the interconnectedness of these metabolic pathways. Understanding HADHB's role in these diseases enhances the potential for targeted therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Mitochondrial trifunctional enzyme catalyzes the last three of the four reactions of the mitochondrial beta-oxidation pathway (PubMed : 29915090, PubMed : 30850536, PubMed : 8135828). The mitochondrial beta-oxidation pathway is the major energy-producing process in tissues and is performed through four consecutive reactions breaking down fatty acids into acetyl-CoA (PubMed : 29915090). Among the enzymes involved in this pathway, the trifunctional enzyme exhibits specificity for long-chain fatty acids (PubMed : 30850536). Mitochondrial trifunctional enzyme is a heterotetrameric complex composed of two proteins, the trifunctional enzyme subunit alpha/HADHA carries the 2,3-enoyl-CoA hydratase and the 3-hydroxyacyl-CoA dehydrogenase activities, while the trifunctional enzyme subunit beta/HADHB described here bears the 3-ketoacyl-CoA thiolase activity (PubMed : 29915090, PubMed : 30850536, PubMed : 8135828).

See full target information HADHB

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 12:1069875 PubMed36518312

2022

Hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit beta gene as a tumour suppressor in stomach adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Li,Jian-Bo Xiong,Zhi-Gang Jie,Hui Xiong

Human molecular genetics 31:3144-3160 PubMed35567413

2022

Mice lacking MBNL1 and MBNL2 exhibit sudden cardiac death and molecular signatures recapitulating myotonic dystrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Kuang-Yung Lee,Carol Seah,Ching Li,Yu-Fu Chen,Chwen-Yu Chen,Ching-I Wu,Po-Cheng Liao,Yu-Chiau Shyu,Hailey R Olafson,Kendra K McKee,Eric T Wang,Chi-Hsiao Yeh,Chao-Hung Wang

Cell death & disease 12:824 PubMed34471096

2021

Cyclic AMP-binding protein Epac1 acts as a metabolic sensor to promote cardiomyocyte lipotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Marion Laudette,Yannis Sainte-Marie,Grégoire Cousin,Dorian Bergonnier,Ismahane Belhabib,Stéphanie Brun,Karina Formoso,Loubna Laib,Florence Tortosa,Camille Bergoglio,Bertrand Marcheix,Jan Borén,Olivier Lairez,Jérémy Fauconnier,Alexandre Lucas,Jeanne Mialet-Perez,Cédric Moro,Frank Lezoualc'h

Cell transplantation 30:9636897211033275 PubMed34338573

2021

CTRP13 Protects H9c2 Cells Against Hypoxia/Reoxygenation (H/R)-Induced Injury Via Regulating the AMPK/Nrf2/ARE Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Weifeng Jiang,Jungang Song,Suitao Zhang,Yanyan Ye,Jun Wang,Yilin Zhang

International journal of molecular sciences 21: PubMed33171690

2020

Pyrroloquinoline Quinone Modifies Lipid Profile, but Not Insulin Sensitivity, of Palmitic Acid-Treated L6 Myotubes.

Applications

Unspecified application

Species

Unspecified reactive species

Elżbieta Supruniuk,Agnieszka Mikłosz,Adrian Chabowski

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-HADHB antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HADHB antibody (AB230667)

Immunocytochemistry/ Immunofluorescence - Anti-HADHB antibody (AB230667)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HADHB antibody (AB230667)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HADHB antibody (AB230667)

Western blot - Anti-HADHB antibody (AB230667)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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