Rabbit Polyclonal HADHB antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human HADHB aa 200-300.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human HADHB aa 200-300. The exact immunogen used to generate this antibody is proprietary information. Database link P55084
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Chimpanzee | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mg of lysate | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Mitochondrial trifunctional enzyme catalyzes the last three of the four reactions of the mitochondrial beta-oxidation pathway (PubMed:29915090, PubMed:30850536, PubMed:8135828). The mitochondrial beta-oxidation pathway is the major energy-producing process in tissues and is performed through four consecutive reactions breaking down fatty acids into acetyl-CoA (PubMed:29915090). Among the enzymes involved in this pathway, the trifunctional enzyme exhibits specificity for long-chain fatty acids (PubMed:30850536). Mitochondrial trifunctional enzyme is a heterotetrameric complex composed of two proteins, the trifunctional enzyme subunit alpha/HADHA carries the 2,3-enoyl-CoA hydratase and the 3-hydroxyacyl-CoA dehydrogenase activities, while the trifunctional enzyme subunit beta/HADHB described here bears the 3-ketoacyl-CoA thiolase activity (PubMed:29915090, PubMed:30850536, PubMed:8135828).
Alternative names
MSTP029, HADHB, TP-beta
Recommended products
Rabbit Polyclonal HADHB antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human HADHB aa 200-300.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human HADHB aa 200-300. The exact immunogen used to generate this antibody is proprietary information. Database link P55084
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein HADHB also called the beta subunit of the Mitochondrial Trifunctional Protein is a part of the enzyme system that breaks down fatty acids. It has an approximate molecular mass of 51 kDa. This protein performs mechanical functions that include enoyl-CoA hydratase hydroxyacyl-CoA dehydrogenase and thiolase activities which facilitate the conversion of fatty acids into acetyl-CoA units. HADHB is expressed in tissues with high energy demands especially in the liver heart and skeletal muscle where it plays an essential role in energy production.
- Biological function summary
The HADHB protein forms a complex with HADHA creating the Mitochondrial Trifunctional Protein. This complex is involved in the inner mitochondrial membrane's beta-oxidation of long-chain fatty acids. Long-chain fatty acids serve as a vital energy source and the HADHB-HADHA complex makes their metabolism more efficient. This process provides ATP essential for maintaining cellular energy homeostasis especially during fasting and intense exercise.
- Pathways
HADHB participates in the fatty acid beta-oxidation pathway a critical component of lipid metabolism. Through this pathway HADHB interacts with proteins like ACADVL and ACADM which catalyze different stages of fatty acid breakdown. Beta-oxidation plays an important role in producing acetyl-CoA subsequently entering the citric acid cycle linking HADHB to energy metabolism. These interactions emphasize the importance of HADHB in maintaining metabolic balance within cells.
- Associated diseases and disorders
HADHB mutations can lead to mitochondrial trifunctional protein deficiency which results in metabolic disorders like fatty acid oxidation disorders and peripheral neuropathy. These conditions impair the body's ability to break down fatty acids affecting energy homeostasis. Disorders related to HADHB may also involve proteins such as CPT1A which transfers fatty acids into mitochondria highlighting the interconnectedness of these metabolic pathways. Understanding HADHB's role in these diseases enhances the potential for targeted therapeutic strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-HADHB antibody (ab240601)
Lysates prepared using NETN lysis buffer.
All lanes: Western blot - Anti-HADHB antibody (ab240601) at 0.1 µg/mL
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 3: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 4: TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg
Lane 5: NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 51 kDa
Exposure time: 10s
Immunoprecipitation - Anti-HADHB antibody (ab240601)
HADHB was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (0.5 or 1 mg for IP, 20% of IP loaded) with ab240601 at 6 μg/reaction. Western blot was performed from the immunoprecipitate using ab240601 at 0.4 μg/ml.
Lane 1: ab240601 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.Detection: Chemiluminescence with exposure time of 10 seconds.
Prepared using NETN lysis buffer.
All lanes: Immunoprecipitation - Anti-HADHB antibody (ab240601)
Predicted band size: 51 kDa
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