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AB189511

Anti-HAI-1 antibody [EPR15652]

4

(1 Review)

|

(8 Publications)

Rabbit Recombinant Monoclonal HAI-1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 8 publications.

View Alternative Names

HAI1, UNQ223/PRO256, SPINT1, Kunitz-type protease inhibitor 1, Hepatocyte growth factor activator inhibitor type 1, HAI-1

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HAI-1 antibody [EPR15652] (AB189511)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HAI-1 antibody [EPR15652] (AB189511)

Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded human transitional cell carcinoma of bladder sections labeling HAI-1 with ab189511 at a 1/100 dilution. Secondary antibody used was a ready to use HRP polymer for rabbit IgG. Counterstained with hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Western blot - Anti-HAI-1 antibody [EPR15652] (AB189511)
  • WB

Supplier Data

Western blot - Anti-HAI-1 antibody [EPR15652] (AB189511)

All lanes:

Western blot - Anti-HAI-1 antibody [EPR15652] (ab189511) at 1/50000 dilution

All lanes:

Human placenta lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 58 kDa

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Western blot - Anti-HAI-1 antibody [EPR15652] (AB189511)
  • WB

Supplier Data

Western blot - Anti-HAI-1 antibody [EPR15652] (AB189511)

All lanes:

Western blot - Anti-HAI-1 antibody [EPR15652] (ab189511) at 1/10000 dilution

All lanes:

HeLa cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 58 kDa

false

Western blot - Anti-HAI-1 antibody [EPR15652] (AB189511)
  • WB

Supplier Data

Western blot - Anti-HAI-1 antibody [EPR15652] (AB189511)

All lanes:

Western blot - Anti-HAI-1 antibody [EPR15652] (ab189511) at 1/50000 dilution

All lanes:

HCT116 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 58 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15652

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Assay Kits

AB288176

Human HAI-1 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human HAI-1 ELISA Kit (AB288176)

  • 0
  • 0
Assay Kits

AB288176

Human HAI-1 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human HAI-1 ELISA Kit (AB288176)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
Primary Antibodies

AB188868

Anti-BAFF-R antibody [EPR14633]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAFF-R antibody [EPR14633] (AB188868)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HAI-1 known also as Hepatocyte Growth Factor Activator Inhibitor 1 or SPINT1 is a type 1 transmembrane protein with a molecular mass of around 55 kDa. This protein commonly expresses in epithelial cells particularly in tissues like the stomach and liver. It serves as a serine protease inhibitor playing a role in regulating the activity of certain proteases. Mouse HAI protein comparable in function and structure is often used in research as a model for understanding HAI-1 in humans. Researchers frequently use HAI detectors and HAI-1 ELISA methods for precise quantification and analysis.
Biological function summary

HAI-1 performs a regulatory role by inhibiting proteases such as matriptase and hepsin which are critical for many cellular functions. As part of a complex HAI-1 binds to these proteases controlling their activity to prevent excessive proteolysis which can damage tissues. This regulatory function is key in maintaining epithelial integrity and homeostasis impacting processes like cell growth and migration. Anti-HAI studies show that disrupting this balance can lead to a cascade of biological alterations.

Pathways

Several cellular processes heavily depend on the balance maintained by HAI-1 in protease activity. One significant pathway involves the HGF/c-Met signaling cascade which impacts cell motility and proliferation. By inhibiting matriptase HAI-1 indirectly affects this pathway regulating cellular responses. Additionally HAI-1 also relates to epithelial-mesenchymal transition (EMT) through controlling proteolytic activity which can influence how cells migrate and differentiate in development or healing.

Deregulation of HAI-1 often links to cancer progression specifically in cases like breast or prostate cancer. In these contexts improper regulation of proteases can lead to enhanced tumor invasiveness and metastasis. HAI-1's interaction with the c-Met receptor pathway facilitated by HGFA illustrates its significant role in cancer biology. Disorders of the liver such as fibrosis also reveal connections where HAI-1 modulation of protease activity affects extracellular matrix turnover implicating HAI-1 in the fibrogenic response alongside proteins like matriptase.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Inhibitor of HGFAC (PubMed : 9045658). Inhibits serine protease activity of ST14/matriptase in vitro (PubMed : 28710277). Inhibits serine protease activity of TMPRSS13, via the BPTI/Kunitz inhibitor 1 domain (PubMed : 20977675).
See full target information SPINT1
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 9:e21616 PubMed38027820

2023

Secreted proteins in plasma and placenta as novel non-invasive biomarkers for intrahepatic cholestasis of pregnancy: A case-control study.

Applications

Unspecified application

Species

Unspecified reactive species

Lingyan Chen,Jingyang Li,Yilan You,Zhiwen Qian,Jiayu Liu,Ying Jiang,Ying Gu,Jianping Xiao,Yan Zhang

Biochimica et biophysica acta. Molecular and cell biology of lipids 1868:159357 PubMed37315736

2023

Characterization of lipoproteins in human placenta and fetal circulation as well as gestational changes in lipoprotein assembly and secretion in human and mouse placentas.

Applications

Unspecified application

Species

Unspecified reactive species

Titli Nargis,Xinhua Lin,Elena Giordano,Laraib Ijaz,Sarah Suhail,Ellen M Gurzenda,Daniel Kiefer,Loredana Quadro,Nazeeh Hanna,M Mahmood Hussain

Cancer science 113:2179-2193 PubMed35332604

2022

Insufficiency of hepatocyte growth factor activator inhibitor-1 confers lymphatic invasion of tongue carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Aya Izumi,Koji Yamamoto,Makiko Kawaguchi,Fumiki Yamashita,Tsuyoshi Fukushima,Takumi Kiwaki,Hiroyuki Tanaka,Yoshihiro Yamashita,Hiroaki Kataoka

PloS one 16:e0252197 PubMed34185790

2021

HAI-1 is an independent predictor of lung cancer mortality and is required for M1 macrophage polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Stanley Borowicz,Daniel R Principe,Matthew J Dorman,Austin J McHenry,Gautam Sondarva,Sandeep Kumar,Vijayalakshmi Ananthanarayanan,Patricia E Simms,Ashley Hess,Ajay Rana

Oncotarget 9:12682-12694 PubMed29560101

2018

Regulation of matriptase and HAI-1 system, a novel therapeutic target in human endometrial cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Pengming Sun,Lifang Xue,Yiyi Song,Xiaodan Mao,Lili Chen,Binhua Dong,Elena Loana Braicu,Jalid Sehouli

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 39:1010428317701636 PubMed28618968

2017

miR-221 and miR-222 synergistically regulate hepatocyte growth factor activator inhibitor type 1 to promote cell proliferation and migration in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Ning,Haiyang Zhang,Xinyi Wang,Shuang Li,Le Zhang,Ting Deng,Likun Zhou,Xia Wang,Rui Liu,Ming Bai,Shaohua Ge,Hongli Li,Dingzhi Huang,Guoguang Ying,Yi Ba

Molecular medicine reports 14:1465-74 PubMed27356668

2016

Decreasing the ratio of matriptase/HAI‑1 by downregulation of matriptase as a potential adjuvant therapy in ovarian cancer.

Applications

WB

Species

Human

Pengming Sun,Zhongqing Jiang,Xiaofang Chen,Lifang Xue,Xiaodan Mao,Guanyu Ruan,Yiyi Song,Alexander Mustea

Oncogene 35:5388-5399 PubMed27109105

2016

Crosstalk between bone marrow-derived myofibroblasts and gastric cancer cells regulates cancer stemness and promotes tumorigenesis.

Applications

IHC-P

Species

Human

Liming Zhu,Xiaojiao Cheng,Jindong Shi,Lin Jiacheng,Gang Chen,Huanyu Jin,Anna B Liu,Hyunseung Pyo,Jing Ye,Yanbo Zhu,Hong Wang,Haoyan Chen,Jingyuan Fang,Li Cai,Timothy C Wang,Chung S Yang,Shui Ping Tu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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