JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB281155

Anti-HAI-1 antibody [EPR23151-84] - BSA and Azide free (Capture)

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal HAI-1 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

HAI1, UNQ223/PRO256, SPINT1, Kunitz-type protease inhibitor 1, Hepatocyte growth factor activator inhibitor type 1, HAI-1

1 Images
Sandwich ELISA - Anti-HAI-1 antibody [EPR23151-84] - BSA and Azide free (Capture) (AB281155)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-HAI-1 antibody [EPR23151-84] - BSA and Azide free (Capture) (AB281155)

Example of Human HAI-1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23151-84

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
style
left-column

Product details

ab281155 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human SPINT1. The recommended pair for sandwich ELISA is:
Capture: ab281155, Human SPINT1 Capture Antibody (unconjugated)
Detector: ab281005, Human SPINT1 Detector Antibody (unconjugated)
The reference range value is 31 - 2000 pg/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HAI-1 known also as Hepatocyte Growth Factor Activator Inhibitor 1 or SPINT1 is a type 1 transmembrane protein with a molecular mass of around 55 kDa. This protein commonly expresses in epithelial cells particularly in tissues like the stomach and liver. It serves as a serine protease inhibitor playing a role in regulating the activity of certain proteases. Mouse HAI protein comparable in function and structure is often used in research as a model for understanding HAI-1 in humans. Researchers frequently use HAI detectors and HAI-1 ELISA methods for precise quantification and analysis.
Biological function summary

HAI-1 performs a regulatory role by inhibiting proteases such as matriptase and hepsin which are critical for many cellular functions. As part of a complex HAI-1 binds to these proteases controlling their activity to prevent excessive proteolysis which can damage tissues. This regulatory function is key in maintaining epithelial integrity and homeostasis impacting processes like cell growth and migration. Anti-HAI studies show that disrupting this balance can lead to a cascade of biological alterations.

Pathways

Several cellular processes heavily depend on the balance maintained by HAI-1 in protease activity. One significant pathway involves the HGF/c-Met signaling cascade which impacts cell motility and proliferation. By inhibiting matriptase HAI-1 indirectly affects this pathway regulating cellular responses. Additionally HAI-1 also relates to epithelial-mesenchymal transition (EMT) through controlling proteolytic activity which can influence how cells migrate and differentiate in development or healing.

Deregulation of HAI-1 often links to cancer progression specifically in cases like breast or prostate cancer. In these contexts improper regulation of proteases can lead to enhanced tumor invasiveness and metastasis. HAI-1's interaction with the c-Met receptor pathway facilitated by HGFA illustrates its significant role in cancer biology. Disorders of the liver such as fibrosis also reveal connections where HAI-1 modulation of protease activity affects extracellular matrix turnover implicating HAI-1 in the fibrogenic response alongside proteins like matriptase.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Inhibitor of HGFAC (PubMed : 9045658). Inhibits serine protease activity of ST14/matriptase in vitro (PubMed : 28710277). Inhibits serine protease activity of TMPRSS13, via the BPTI/Kunitz inhibitor 1 domain (PubMed : 20977675).
See full target information SPINT1
style
left-column
style
left-column
style
right-column

Complementary Products

Assay Kits

AB288176

Human HAI-1 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human HAI-1 ELISA Kit (AB288176)

  • 0
  • 0
Assay Kits

AB288176

Human HAI-1 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human HAI-1 ELISA Kit (AB288176)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
Primary Antibodies

AB188868

Anti-BAFF-R antibody [EPR14633]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAFF-R antibody [EPR14633] (AB188868)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com