AB16113

Anti-hair cortex Cytokeratin/K40 antibody [AE13]

Name: Anti-hair cortex Cytokeratin/K40 antibody [AE13] (ab16113) | Abcam
Brand: Abcam Limited
SKU: AB16113
Rating: 4 (7 reviews)

(7 Reviews)

(45 Publications)

Mouse Monoclonal hair cortex Cytokeratin/K40 antibody. Suitable for Flow Cyt, WB, IHC-P, IHC-Fr and reacts with Human, Mouse samples. Cited in 45 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human KRT40.

View Alternative Names

KA36, KRT40, Cytokeratin-40, Keratin-40, Type I hair keratin Ka36, CK-40, K40

2 Images

Flow Cytometry - Anti-hair cortex Cytokeratin/K40 antibody [AE13] (AB16113)

Flow Cyt

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Flow Cytometry - Anti-hair cortex Cytokeratin/K40 antibody [AE13] (AB16113)

Overlay histogram showing A431 cells stained with ab16113 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16113, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A431 cells fixed with 100% methanol (5 min)/permeabilized in 0.1% PBS-Triton used under the same conditions.

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Western blot - Anti-hair cortex Cytokeratin/K40 antibody [AE13] (AB16113)

All lanes:

Western blot - Anti-hair cortex Cytokeratin/K40 antibody [AE13] (ab16113) at 1 µg/mL

Lane 1:

Western blot - Mouse skin tissue lysate (14 days) (<a href='/en-us/products/tissue-lysates/mouse-skin-tissue-lysate-14-days-ab7271'>ab7271</a>) at 10 µg

Lane 2:

Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/5000 dilution

Predicted band size: 48 kDa

Observed band size: 25 kDa,44 kDa,55 kDa

true

Exposure time: 20min

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

AE13

Isotype

IgG1

Carrier free

Reacts with

Mouse, Human

Applications

WB, Flow Cyt, IHC-Fr, IHC-P

applications

Immunogen

Native Full Length Protein corresponding to Human KRT40.

Q6A162

Specificity

This antibody is specific for the family of hair cortex keratins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/20 - 1/50", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Product details

This antibody is an excellent marker for hair and nail differentiation.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.2 Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hair cortex Cytokeratin also known as K40 is a type of intermediate filament protein with a molecular mass of approximately 40 kDa. It is an important structural component in the hair shaft particularly within the cortex where it contributes to the mechanical properties of hair fiber. K40 is predominantly expressed in keratinocytes found in the hair follicle's inner layers including the scalp. This protein provides toughness and elasticity to the hair and it plays a significant role in maintaining hair integrity.

Biological function summary

Cytokeratin K40 helps form a stable cytoskeletal network that supports the structural framework of the hair shaft. Within the keratin family K40 is part of a larger complex of keratin proteins that interact to form strong fibrous structures. It partners with other keratins like AE13 to reinforce the mechanical durability of hair. This complex network supports the rigidity and resilience necessary to resist environmental damage and mechanical stress.

Pathways

The function of hair cortex Cytokeratin K40 interweaves with processes involved in epithelial cell differentiation and hair formation. K40 aligns with the keratinization pathway where it collaborates with related keratins to implement the transformation of keratinocytes into the keratin-rich hair cortex. This pathway connects K40 with proteins like AE1 and AE13 which together facilitate the assembly of a robust cytoskeletal matrix essential for forming healthy hair.

Alterations in Cytokeratin K40 expression can relate to hair shaft disorders and conditions like alopecia. These diseases often arise when the normal architecture and strength of hair fibers are compromised. Within this context changes in K40 may also impact the activity of other keratin proteins such as b and AE13 exacerbating the severity of hair brittleness and growth issues. Understanding the role of K40 may help develop therapeutic strategies to manage or treat associated hair disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May play a role in late hair differentiation.

See full target information KRT40

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Publications (45)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eadx4511 PubMed40938992

2025

The citrullinating enzyme PADI4 governs progenitor cell proliferation and translation in developing hair follicles.

Applications

Unspecified application

Species

Unspecified reactive species

Kim Vikhe Patil,Nil Campamà Sanz,Kylie Hin-Man Mak,Wei Yang,Karl Annusver,Jasson Makkar,David Grommisch,Li Lei,Priyanka Sharma,Ryan R Driskell,Maria Kasper,Maria Genander

Biofabrication 16: PubMed38941996

2024

A biopsy-sized 3D skin model with a perifollicular vascular plexus enables studying immune cell trafficking in the skin.

Applications

Unspecified application

Species

Unspecified reactive species

Krutav Rakesh Shah,Laura Garriga-Cerda,Alberto Pappalardo,Leila Sorrells,Hun Jin Jeong,Chang H Lee,Hasan Erbil Abaci

Biomaterials research 28:0018 PubMed40630836

2024

Three-Dimensional Cultured Human Dermal Papilla Cells in HGC-Coated Environments Enhance Hair Regeneration and Artificial Skin Integration.

Applications

Unspecified application

Species

Unspecified reactive species

Thuy Trang Truong,Heejin Park,Kyoung Hwan Park,Jin Jung Song,Byoung-Seok Lee,Kang Moo Huh,Sun-Woong Kang

Nature communications 14:4820 PubMed37563109

2023

Hdac1 and Hdac2 regulate the quiescent state and survival of hair-follicle mesenchymal niche.

Applications

Unspecified application

Species

Unspecified reactive species

Hadas Sibony-Benyamini,Emil Aamar,David Enshell-Seijffers

Scientific reports 13:4847 PubMed36964149

2023

In vitro hair follicle growth model for drug testing.

Applications

Unspecified application

Species

Unspecified reactive species

Tatsuto Kageyama,Hikaru Miyata,Jieun Seo,Ayaka Nanmo,Junji Fukuda

Molecular therapy. Nucleic acids 32:94-110 PubMed37020681

2023

Mechanical stimuli-induced CCL2 restores adult mouse cells to regenerate hair follicles.

Applications

Unspecified application

Species

Unspecified reactive species

Wang Wu,Wei Zhou,Jingwei Jiang,Mengyue Wang,Jinwei Zhang,Jing Yang,Qu Tang,Huawen Liu,Deming Liu,Wei Xu,Julia Li Zhong,Li Yang,Mingxing Lei

Cells 12: PubMed36831222

2023

CXXC5 Mediates DHT-Induced Androgenetic Alopecia via PGD.

Applications

Unspecified application

Species

Unspecified reactive species

Yeong Chan Ryu,Jiyeon Park,You-Rin Kim,Sehee Choi,Geon-Uk Kim,Eunhwan Kim,Yumi Hwang,Heejene Kim,Gyoonhee Han,Soung-Hoon Lee,Kang-Yell Choi

iScience 25:105150 PubMed36193049

2022

Wnt-activating human skin organoid model of atopic dermatitis induced by and its protective effects by .

Applications

Unspecified application

Species

Unspecified reactive species

Song-Yi Jung,Hyun Ju You,Min-Ji Kim,GwangPyo Ko,Seunghee Lee,Kyung-Sun Kang

Annals of translational medicine 10:767 PubMed35965801

2022

Self-assembled complete hair follicle organoids by coculture of neonatal mouse epidermal cells and dermal cells in Matrigel.

Applications

Unspecified application

Species

Unspecified reactive species

Sitian Xie,Liyun Chen,Mingjun Zhang,Cuiping Zhang,Haihong Li

Dermatology (Basel, Switzerland) 239:60-71 PubMed35843211

2022

Effects of NCSTN Mutation on Hair Follicle Components in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Tian-Wei Shi,Wei Cao,Qing-Zan Zhao,Hong-Xing Yu,Si-Sen Zhang,Yi-Bin Hao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-hair cortex Cytokeratin/K40 antibody [AE13]

Flow Cytometry - Anti-hair cortex Cytokeratin/K40 antibody [AE13] (AB16113)

Western blot - Anti-hair cortex Cytokeratin/K40 antibody [AE13] (AB16113)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (45)

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