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Mouse Recombinant Monoclonal hair cortex Cytokeratin/K40 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat samples.

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Images

Western blot - Anti-hair cortex Cytokeratin/K40 antibody [AE13] (AB289545), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hair cortex Cytokeratin/K40 antibody [AE13] (AB289545), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hair cortex Cytokeratin/K40 antibody [AE13] (AB289545), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hair cortex Cytokeratin/K40 antibody [AE13] (AB289545), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hair cortex Cytokeratin/K40 antibody [AE13] (AB289545), expandable thumbnail

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBIHC-PIHC-FrICC/IF
Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Tested
Tested
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Tested
Tested
Not recommended
Not recommended

Not recommended
Not recommended

Species
Human, Rat, Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Rat, Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Rat, Mouse
Dilution info
-
Notes

-

Target data

Function

May play a role in late hair differentiation.

Alternative names

KA36, KRT40, Cytokeratin-40, Keratin-40, Type I hair keratin Ka36, CK-40, K40

Recommended products

Mouse Recombinant Monoclonal hair cortex Cytokeratin/K40 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat samples.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
AE13
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Hair cortex Cytokeratin also known as K40 is a type of intermediate filament protein with a molecular mass of approximately 40 kDa. It is an important structural component in the hair shaft particularly within the cortex where it contributes to the mechanical properties of hair fiber. K40 is predominantly expressed in keratinocytes found in the hair follicle's inner layers including the scalp. This protein provides toughness and elasticity to the hair and it plays a significant role in maintaining hair integrity.

Biological function summary

Cytokeratin K40 helps form a stable cytoskeletal network that supports the structural framework of the hair shaft. Within the keratin family K40 is part of a larger complex of keratin proteins that interact to form strong fibrous structures. It partners with other keratins like AE13 to reinforce the mechanical durability of hair. This complex network supports the rigidity and resilience necessary to resist environmental damage and mechanical stress.

Pathways

The function of hair cortex Cytokeratin K40 interweaves with processes involved in epithelial cell differentiation and hair formation. K40 aligns with the keratinization pathway where it collaborates with related keratins to implement the transformation of keratinocytes into the keratin-rich hair cortex. This pathway connects K40 with proteins like AE1 and AE13 which together facilitate the assembly of a robust cytoskeletal matrix essential for forming healthy hair.

Associated diseases and disorders

Alterations in Cytokeratin K40 expression can relate to hair shaft disorders and conditions like alopecia. These diseases often arise when the normal architecture and strength of hair fibers are compromised. Within this context changes in K40 may also impact the activity of other keratin proteins such as b and AE13 exacerbating the severity of hair brittleness and growth issues. Understanding the role of K40 may help develop therapeutic strategies to manage or treat associated hair disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-hair cortex Cytokeratin/K40 antibody [AE13] (ab289545), expandable thumbnail

    Western blot - Anti-hair cortex Cytokeratin/K40 antibody [AE13] (ab289545)

    Blocking and diluting buffer: 5% NFDM/TBST

    Exposure time: 10 seconds

    All lanes: Western blot - Anti-hair cortex Cytokeratin/K40 antibody [AE13] (ab289545) at 1/1000 dilution

    Lane 1: Mouse skin tissue lysate at 20 µg

    Lane 2: Mouse brain tissue lysate at 20 µg

    Lane 3: Rat skin tissue lysate at 20 µg

    Lane 4: Rat brain tissue lysate at 20 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 48 kDa

    Observed band size: 48 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hair cortex Cytokeratin/K40 antibody [AE13] (ab289545), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hair cortex Cytokeratin/K40 antibody [AE13] (ab289545)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue labeling hair cortex Cytokeratin/K40 with ab289545 at 1/500 (1.032 ug/ml) dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879). Positive staining on mouse skin (PMID:2432071). The section was incubated with ab289545 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hair cortex Cytokeratin/K40 antibody [AE13] (ab289545), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hair cortex Cytokeratin/K40 antibody [AE13] (ab289545)

    Immunohistochemical analysis of paraffin-embedded rat skin tissue labeling hair cortex Cytokeratin/K40 with ab289545 at 1/500 (1.032 ug/ml) dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879). Positive staining on rat skin. The section was incubated with ab289545 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hair cortex Cytokeratin/K40 antibody [AE13] (ab289545), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hair cortex Cytokeratin/K40 antibody [AE13] (ab289545)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling hair cortex Cytokeratin/K40 with ab289545 at 1/500 (1.032 ug/ml) dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879). The section was incubated with ab289545 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Negative Control: No staining on mouse cerebrum.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hair cortex Cytokeratin/K40 antibody [AE13] (ab289545), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hair cortex Cytokeratin/K40 antibody [AE13] (ab289545)

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling hair cortex Cytokeratin/K40 with ab289545 at 500 (1.032 ug/ml) dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879). The section was incubated with ab289545 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Negative Control: No staining on rat cerebrum.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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