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AB289545

Anti-hair cortex Cytokeratin/K40 antibody [AE13]

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Mouse Recombinant Monoclonal hair cortex Cytokeratin/K40 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat samples.

View Alternative Names

KA36, KRT40, Cytokeratin-40, Keratin-40, Type I hair keratin Ka36, CK-40, K40

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hair cortex Cytokeratin/K40 antibody [AE13] (AB289545)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hair cortex Cytokeratin/K40 antibody [AE13] (AB289545)

Immunohistochemical analysis of paraffin-embedded rat skin tissue labeling hair cortex Cytokeratin/K40 with ab289545 at 1/500 (1.032 ug/ml) dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Positive staining on rat skin. The section was incubated with ab289545 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hair cortex Cytokeratin/K40 antibody [AE13] (AB289545)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hair cortex Cytokeratin/K40 antibody [AE13] (AB289545)

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling hair cortex Cytokeratin/K40 with ab289545 at 500 (1.032 ug/ml) dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). The section was incubated with ab289545 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Negative Control : No staining on rat cerebrum.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hair cortex Cytokeratin/K40 antibody [AE13] (AB289545)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hair cortex Cytokeratin/K40 antibody [AE13] (AB289545)

Immunohistochemical analysis of paraffin-embedded mouse skin tissue labeling hair cortex Cytokeratin/K40 with ab289545 at 1/500 (1.032 ug/ml) dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Positive staining on mouse skin (PMID : 2432071). The section was incubated with ab289545 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hair cortex Cytokeratin/K40 antibody [AE13] (AB289545)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hair cortex Cytokeratin/K40 antibody [AE13] (AB289545)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling hair cortex Cytokeratin/K40 with ab289545 at 1/500 (1.032 ug/ml) dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). The section was incubated with ab289545 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Negative Control : No staining on mouse cerebrum.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Western blot - Anti-hair cortex Cytokeratin/K40 antibody [AE13] (AB289545)
  • WB

Supplier Data

Western blot - Anti-hair cortex Cytokeratin/K40 antibody [AE13] (AB289545)

Blocking and diluting buffer : 5% NFDM/TBST

Exposure time : 10 seconds

All lanes:

Western blot - Anti-hair cortex Cytokeratin/K40 antibody [AE13] (ab289545) at 1/1000 dilution

Lane 1:

Mouse skin tissue lysate at 20 µg

Lane 2:

Mouse brain tissue lysate at 20 µg

Lane 3:

Rat skin tissue lysate at 20 µg

Lane 4:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 48 kDa

Observed band size: 48 kDa

false

  • Carrier free

    Anti-hair cortex Cytokeratin/K40 antibody [AE13] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

AE13

Isotype

IgG1

Carrier free

No

Reacts with

Rat, Mouse

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hair cortex Cytokeratin also known as K40 is a type of intermediate filament protein with a molecular mass of approximately 40 kDa. It is an important structural component in the hair shaft particularly within the cortex where it contributes to the mechanical properties of hair fiber. K40 is predominantly expressed in keratinocytes found in the hair follicle's inner layers including the scalp. This protein provides toughness and elasticity to the hair and it plays a significant role in maintaining hair integrity.
Biological function summary

Cytokeratin K40 helps form a stable cytoskeletal network that supports the structural framework of the hair shaft. Within the keratin family K40 is part of a larger complex of keratin proteins that interact to form strong fibrous structures. It partners with other keratins like AE13 to reinforce the mechanical durability of hair. This complex network supports the rigidity and resilience necessary to resist environmental damage and mechanical stress.

Pathways

The function of hair cortex Cytokeratin K40 interweaves with processes involved in epithelial cell differentiation and hair formation. K40 aligns with the keratinization pathway where it collaborates with related keratins to implement the transformation of keratinocytes into the keratin-rich hair cortex. This pathway connects K40 with proteins like AE1 and AE13 which together facilitate the assembly of a robust cytoskeletal matrix essential for forming healthy hair.

Alterations in Cytokeratin K40 expression can relate to hair shaft disorders and conditions like alopecia. These diseases often arise when the normal architecture and strength of hair fibers are compromised. Within this context changes in K40 may also impact the activity of other keratin proteins such as b and AE13 exacerbating the severity of hair brittleness and growth issues. Understanding the role of K40 may help develop therapeutic strategies to manage or treat associated hair disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May play a role in late hair differentiation.
See full target information KRT40
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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