Anti-hair cortex Cytokeratin/K40 antibody [AE13] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hair cortex Cytokeratin/K40 antibody [AE13] - BSA and Azide free (AB255963)

This data was developed using ab289545 the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling hair cortex Cytokeratin/K40 with ab289545 at 500 (1.032 ug/ml) dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). The section was incubated with ab289545 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Negative Control : No staining on rat cerebrum.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hair cortex Cytokeratin/K40 antibody [AE13] - BSA and Azide free (AB255963)

This data was developed using the same antibody clone in a different buffer formulation (ab289545).

Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat skin labeling hair cortex Cytokeratin with ab289545 at 1/500 dilution. A Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) was used as the secondary antibody. Counterstained with Hematoxylin. Negative control also shown.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Positive staining on rat skin. The section was incubated with ab289545 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hair cortex Cytokeratin/K40 antibody [AE13] - BSA and Azide free (AB255963)

This data was developed using the same antibody clone in a different buffer formulation (ab289545).

Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse skin labeling hair cortex Cytokeratin with ab289545 at 1/500 dilution. A Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) was used as the secondary antibody. Counterstained with Hematoxylin. Negative control also shown.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Positive staining on mouse skin. The section was incubated with ab289545 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hair cortex Cytokeratin/K40 antibody [AE13] - BSA and Azide free (AB255963)

This data was developed using ab289545 the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling hair cortex Cytokeratin/K40 with ab289545 at 1/500 (1.032 ug/ml) dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). The section was incubated with ab289545 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Negative Control : No staining on mouse cerebrum.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• WB

Lab

Western blot - Anti-hair cortex Cytokeratin/K40 antibody [AE13] - BSA and Azide free (AB255963)

This data was developed using the same antibody clone in a different buffer formulation (ab289545)

Blocking buffer and concentration : 5% NFDM/TBST

Diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-hair cortex Cytokeratin/K40 antibody [AE13] - BSA and Azide free (ab255963) at 1/1000 dilution

Lane 1:

Mouse skin tissue lysate at 20 µg

Lane 2:

Mouse brain tissue lysate at 20 µg

Lane 3:

Rat skin tissue lysate at 20 µg

Lane 4:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 48 kDa

Observed band size: 48 kDa

false

Exposure time: 10s