JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB779

Anti-Hairy Cell Leukemia antibody [DBA.44]

Be the first to review this product! Submit a review

|

(3 Publications)

Mouse Monoclonal Hairy Cell Leukemia antibody. Suitable for IHC-P, IHC-Fr and reacts with Human samples. Cited in 3 publications.
1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hairy Cell Leukemia antibody [DBA.44] (AB779)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hairy Cell Leukemia antibody [DBA.44] (AB779)

Formalin fixed paraffin embedded human tonsil tissue, staining Hairy Cell Leukemia with ab779 in immunohistochemical analysis

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

DBA.44

Isotype

IgM

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

IHC-Fr, IHC-P

applications

style
left-column

Alternative Products

Primary Antibodies

AB857

Anti-Hairy Cell Leukemia antibody [DBA.44], prediluted

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hairy Cell Leukemia antibody [DBA.44], prediluted (AB857)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB857

Anti-Hairy Cell Leukemia antibody [DBA.44], prediluted

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hairy Cell Leukemia antibody [DBA.44], prediluted (AB857)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB182858

Anti-Bcl-2 antibody [EPR17509]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [EPR17509] (AB182858)

  • 5
  • 13
Primary Antibodies

AB7291

Anti-alpha Tubulin antibody [DM1A] - Loading Control

BOND RX™ Validated

Western blot - Anti-alpha Tubulin antibody [DM1A] - Loading Control (AB7291)

  • 5
  • 92
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/15 - 1/30", "IHCP-species-notes": "<p>Primary incubation for 1 hour at room temperature.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.3 - 7.5 Preservative: 0.05% Sodium azide Constituents: Tissue culture supernatant, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hairy Cell Leukemia (HCL) is a rare type of chronic lymphocytic leukemia. It involves an abnormal proliferation of B lymphocytes which present with hair-like cytoplasmic projections. The disease is named for the hair-like cells that appear due to the presence of these projections. HCL is primarily found in the blood and bone marrow. These cells express a distinct immunophenotype with co-expression of CD11c CD25 CD103 and DBA.44 antigens making it recognizable through flow cytometry. HCL cells also carry a weight of approximately 60 kDa. The aberrant B cells infiltrate the bone marrow leading to fibrosis and subsequent pancytopenia.
Biological function summary

HCL affects the immune system by impairing normal B cell function. These abnormal cells fail to produce normal antibodies leading to weakened immune responses. The leukemia cells often accumulate in the spleen and liver causing organomegaly. Hairy cells interfere with hematopoiesis by occupying spaces in the bone marrow. Despite their abnormal nature they require support from the microenvironment for survival. HCL cells commonly exist as part of a complex with extracellular matrix proteins that help their adhesion and survival.

Pathways

HCL is tightly linked with the B-cell receptor (BCR) signaling pathway. The activation of BCR plays a significant role in the survival and proliferation of these malignant cells. HCL also involves the downstream pathway of the MAPK/ERK signaling cascade important for cell proliferation and survival. Proteins like spleen tyrosine kinase (SYK) are engaged actively in these pathways. The interaction between SYK and BCR signaling is essential for the pathogenesis of HCL as it promotes cell survival and proliferation.

Hairy cell leukemia is linked closely to immunosuppression and increased susceptibility to infections. This is due to the ineffective immune response stemming from the dysfunctional B cells. Furthermore HCL is related to autoimmune disorders as the compromised immune surveillance can lead to autoantibody production. Clinically hairy cell leukemia is associated with the malfunction of proteins like BRAF where mutations in the BRAF gene particularly the V600E mutation are commonly observed in patients with HCL. This mutation provides targets for therapeutic interventions such as BRAF inhibitors which specifically aim to exploit the mutated pathways for treatment.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

See full target information Hairy Cell Leukemia
style
left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Brain research bulletin 155:67-80 PubMed31756421

2019

Toll-like receptor 4 (TLR4) influences the glial reaction in the spinal cord and the neural response to injury following peripheral nerve crush.

Applications

Unspecified application

Species

Unspecified reactive species

Patrícia Ribeiro,Mateus V Castro,Matheus Perez,Luciana P Cartarozzi,Aline B Spejo,Gabriela B Chiarotto,Taize M Augusto,Alexandre L R Oliveira

Neural plasticity 2016:2932784 PubMed27642524

2016

Direct Spinal Ventral Root Repair following Avulsion: Effectiveness of a New Heterologous Fibrin Sealant on Motoneuron Survival and Regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Mateus Vidigal de Castro,Roberta Barbizan,Rui Seabra Ferreira,Benedito Barraviera,Alexandre Leite Rodrigues de Oliveira

Blood 74:2476-85 PubMed2679917

1989

Production of anti-B monoclonal antibodies (DBB.42, DBA.44, DNA.7, and DND.53) reactive on paraffin-embedded tissues with a new B-lymphoma cell line grafted into athymic nude mice.

Applications

Unspecified application

Species

Unspecified reactive species

T al Saati,S Caspar,P Brousset,S Chittal,P Caverivière,H Hounieu,N Dastugue,J B Idoipe,J Icart,C Mazerolles
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com