AB279390

Anti-HAND1 antibody [OTI1G10]

Name: Anti-HAND1 antibody [OTI1G10] (ab279390) | Abcam
Brand: Abcam Limited
SKU: AB279390
Rating: 5 (1 reviews)

(1 Review)

(1 Publication)

Mouse Monoclonal HAND1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Recombinant full length protein - Human, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human HAND1.

View Alternative Names

BHLHA27, EHAND, HAND1, Heart- and neural crest derivatives-expressed protein 1, Class A basic helix-loop-helix protein 27, bHLHa27, eHAND

4 Images

Flow Cytometry (Intracellular) - Anti-HAND1 antibody [OTI1G10] (AB279390)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-HAND1 antibody [OTI1G10] (AB279390)

Flow cytometric Analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cells using ab279390 at a 1/100 dilution (Red), compared to a non-specific negative control antibody (Blue).

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-HAND1 antibody [OTI1G10] (AB279390)

Flow cytometric Analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells using ab279390 at a 1/100 dilution (Red), compared to a non-specific negative control antibody (Blue).

Supplier Data

Western blot - Anti-HAND1 antibody [OTI1G10] (AB279390)

All lanes:

Western blot - Anti-HAND1 antibody [OTI1G10] (ab279390) at 1/500 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with the pCMV6-ENTRY control at 5 µg

Lane 2:

HEK-293T transfected cells pCMV6-ENTRY HAND1 cDNA for 48 hrs and lysed at 5 µg

Predicted band size: 24 kDa

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Supplier Data

Western blot - Anti-HAND1 antibody [OTI1G10] (AB279390)

All lanes:

Western blot - Anti-HAND1 antibody [OTI1G10] (ab279390) at 1/200 dilution

Lane 1:

Testis tissue extracts at 10 µg

Lane 2:

Omentum tissue extracts at 10 µg

Lane 3:

Uterus tissue extract at 10 µg

Lane 4:

Breast tissue extract at 10 µg

Lane 5:

Brain tissue extract at 10 µg

Lane 6:

Liver tissue extract at 10 µg

Lane 7:

Ovary tissue extract at 10 µg

Lane 8:

Thyroid gland tissue extract at 10 µg

Lane 9:

Colon tissue extract at 10 µg

Predicted band size: 24 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI1G10

Isotype

IgG2b

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human HAND1.

O96004

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

Purified from TCS.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HAND1 also known as eHAND or Thing1 is a transcription factor with a mass of approximately 23 kDa. It belongs to the basic helix-loop-helix (bHLH) family of transcription factors and acts by binding to DNA sequences to regulate gene expression. HAND1 plays a role in dimer formation usually forming heterodimers with other bHLH proteins. This target expresses predominantly in the heart and in extraembryonic tissues such as the placenta and trophoblast. During various stages of development HAND1 shows different expression patterns depending on the tissue type.

Biological function summary

The function of HAND1 involves critical roles in differentiation and development especially in cardiac development and placental formation. HAND1 is part of a transcriptional complex that guides embryonic cells into specific lineages. It influences cardiomyocyte proliferation and contributes to the structural formation of the developing heart. In placental tissues HAND1 controls trophoblast differentiation which is essential for proper placenta development and function.

Pathways

HAND1 interacts with several biological pathways notably the Wnt signaling pathway and TGF-beta signaling pathway. These pathways control cell proliferation differentiation and migration which are important for development. HAND1 forms functional relationships with other proteins such as HAND2 during these processes ensuring proper transcriptional regulation needed for developmental decisions. Wnt signaling can modulate HAND1 activity indirectly by influencing the expression of dimer partners.

HAND1 relates to congenital heart defects and pre-eclampsia. Variations in HAND1 expression or mutations can disrupt normal cardiac morphogenesis leading to structural heart issues present at birth. In placental disorders like pre-eclampsia altered HAND1 expression can affect trophoblast differentiation and placental function. The interaction with other proteins such as GATA4 highlights its role in pathogenesis. Understanding HAND1's function and regulatory mechanisms provides insights into the development of these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor that plays an essential role in both trophoblast giant cell differentiation and in cardiac morphogenesis (By similarity). Binds the DNA sequence 5'-NRTCTG-3' (non-canonical E-box) (By similarity). Acts as a transcriptional repressor of SOX15 (By similarity). In the adult, could be required for ongoing expression of cardiac-specific genes (PubMed : 9931445).

See full target information HAND1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell biology international 46:1144-1155 PubMed35293659

2022

KLF11 promotes the progression of glioma via regulating Holliday junction recognition protein.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Li,Chunhong Wang,Rui Cheng,Haiyang Su,Lijun Wang,Lei Ji,Hongming Ji

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HAND1 antibody [OTI1G10]

Flow Cytometry (Intracellular) - Anti-HAND1 antibody [OTI1G10] (AB279390)

Flow Cytometry (Intracellular) - Anti-HAND1 antibody [OTI1G10] (AB279390)

Western blot - Anti-HAND1 antibody [OTI1G10] (AB279390)

Western blot - Anti-HAND1 antibody [OTI1G10] (AB279390)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

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