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AB229817

Anti-HAO2 antibody

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(1 Publication)

Rabbit Polyclonal HAO2 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human HAO2 aa 1-200.

View Alternative Names

HAOX2, GIG16, HAO2, 2-Hydroxyacid oxidase 2, Cell growth-inhibiting gene 16 protein, Long chain alpha-hydroxy acid oxidase, Long-chain L-2-hydroxy acid oxidase

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HAO2 antibody (AB229817)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HAO2 antibody (AB229817)

Paraffin-embedded human kidney tissue stained for HAO2 using ab229817 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-HAO2 antibody (AB229817)
  • WB

Supplier Data

Western blot - Anti-HAO2 antibody (AB229817)

All lanes:

Western blot - Anti-HAO2 antibody (ab229817) at 1/500 dilution

Lane 1:

MCF7 (human breast adenocarcinoma cell line) lysate

Lane 2:

Rat kidney lysate

Lane 3:

Mouse kidney lysate

Secondary

All lanes:

Goat anti-rabbit IgG at 1/50000 dilution

Predicted band size: 39 kDa

Observed band size: 39 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HAO2 antibody (AB229817)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HAO2 antibody (AB229817)

Paraffin-embedded human liver tissue stained for HAO2 using ab229817 at 1/100 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human HAO2 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q9NYQ3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The HAO2 protein also known as hydroxyacid oxidase 2 plays an enzyme role in the cellular environment. It catalyzes the oxidation of glycolate to glyoxylate which involves the substrate binding and conversion. With a molecular mass approximately 40 kDa HAO2 expresses mainly in tissues like liver and pancreas where it supports metabolic processes. Understanding its precise mechanical role helps clarify its action in metabolic pathways.
Biological function summary

HAO2 functions as a significant part of the peroxisomal beta-oxidation process. It does not seem to act as a component of a larger protein complex; it rather operates independently in modifying substrates. Its actions contribute to the regulation of cellular pH and reactive oxygen species through its enzymatic reactions. HAO2's activity impacts cellular energy balance by participating in specific metabolic processes involving medium-chain hydroxy acids.

Pathways

HAO2 integrates into the metabolic pathway of glyoxylate and dicarboxylate metabolism where it executes important reactions involving hydroxy acids. In addition HAO2 co-functions with proteins such as lactate dehydrogenase in the related energy metabolism pathways aiding in energy production and balance within cells. These interconnected pathways exhibit how HAO2 supports metabolic function and stability.

HAO2 shows relevance in metabolic disorders such as primary hyperoxaluria. This condition results from an overproduction of oxalate due to defective substrate metabolism where HAO2 acts. Furthermore aberrant HAO2 function associates with renal issues due to its role in oxalate metabolism. In this context HAO2 has an indirect connection to proteins like alanine-glyoxylate aminotransferase which also impacts oxalate metabolism linking them through the biological disorder framework.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Oxidase that catalyzes the oxidation of medium and long chain hydroxyacids such as 2-hydroxyhexadecanoate and 2-hydroxyoctanoate, to the correspondong 2-oxoacids (PubMed : 10777549). Its role in the oxidation of 2-hydroxy fatty acids may contribute to the general pathway of fatty acid alpha-oxidation (Probable). Active in vitro with the artificial electron acceptor 2,6-dichlorophenolindophenol (DCIP), but O2 is believed to be the physiological electron acceptor, leading to the production of H2O2. Is not active on glycolate, glyoxylate, L-lactate and 2-hydroxybutanoate (PubMed : 10777549).
See full target information HAO2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 12:911715 PubMed35795047

2022

CircASPH Promotes Hepatocellular Carcinoma Progression Through Methylation and Expression of HAO2.

Applications

Unspecified application

Species

Unspecified reactive species

Han Zhuo,Jinguo Xia,Jin Zhang,Junwei Tang,Sheng Han,Qitong Zheng,Deming Zhu,Feihong Zhang,Zhenggang Xu,Dongwei Sun,Zhongming Tan,Chen Wu
View all publications
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