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Rabbit Polyclonal HAO2 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Hydroxyacid oxidase 2 aa 1-200.

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Images

Western blot - Anti-HAO2 antibody (AB229817), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HAO2 antibody (AB229817), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HAO2 antibody (AB229817), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human Hydroxyacid oxidase 2 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NYQ3

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested
Mouse
Tested
Expected
Rat
Tested
Expected

Tested
Tested

Species

Human

Dilution info

1/500 - 1/5000

Notes

-

Species

Mouse

Dilution info

1/500 - 1/5000

Notes

-

Species

Rat

Dilution info

1/500 - 1/5000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/20 - 1/200

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Target data

Function

Oxidase that catalyzes the oxidation of medium and long chain hydroxyacids such as 2-hydroxyhexadecanoate and 2-hydroxyoctanoate, to the correspondong 2-oxoacids (PubMed:10777549). Its role in the oxidation of 2-hydroxy fatty acids may contribute to the general pathway of fatty acid alpha-oxidation (Probable). Active in vitro with the artificial electron acceptor 2,6-dichlorophenolindophenol (DCIP), but O2 is believed to be the physiological electron acceptor, leading to the production of H2O2. Is not active on glycolate, glyoxylate, L-lactate and 2-hydroxybutanoate (PubMed:10777549).

Alternative names

Recommended products

Rabbit Polyclonal HAO2 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Hydroxyacid oxidase 2 aa 1-200.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human Hydroxyacid oxidase 2 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NYQ3
Purification technique

Affinity purification Protein G

Concentration
Loading...
Purification notes

Purity >95%.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The HAO2 protein also known as hydroxyacid oxidase 2 plays an enzyme role in the cellular environment. It catalyzes the oxidation of glycolate to glyoxylate which involves the substrate binding and conversion. With a molecular mass approximately 40 kDa HAO2 expresses mainly in tissues like liver and pancreas where it supports metabolic processes. Understanding its precise mechanical role helps clarify its action in metabolic pathways.

Biological function summary

HAO2 functions as a significant part of the peroxisomal beta-oxidation process. It does not seem to act as a component of a larger protein complex; it rather operates independently in modifying substrates. Its actions contribute to the regulation of cellular pH and reactive oxygen species through its enzymatic reactions. HAO2’s activity impacts cellular energy balance by participating in specific metabolic processes involving medium-chain hydroxy acids.

Pathways

HAO2 integrates into the metabolic pathway of glyoxylate and dicarboxylate metabolism where it executes important reactions involving hydroxy acids. In addition HAO2 co-functions with proteins such as lactate dehydrogenase in the related energy metabolism pathways aiding in energy production and balance within cells. These interconnected pathways exhibit how HAO2 supports metabolic function and stability.

Associated diseases and disorders

HAO2 shows relevance in metabolic disorders such as primary hyperoxaluria. This condition results from an overproduction of oxalate due to defective substrate metabolism where HAO2 acts. Furthermore aberrant HAO2 function associates with renal issues due to its role in oxalate metabolism. In this context HAO2 has an indirect connection to proteins like alanine-glyoxylate aminotransferase which also impacts oxalate metabolism linking them through the biological disorder framework.

Product promise

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3 product images

  • Western blot - Anti-HAO2 antibody (ab229817), expandable thumbnail

    Western blot - Anti-HAO2 antibody (ab229817)

    All lanes: Western blot - Anti-HAO2 antibody (ab229817) at 1/500 dilution

    Lane 1: MCF7 (human breast adenocarcinoma cell line) lysate

    Lane 2: Rat kidney lysate

    Lane 3: Mouse kidney lysate

    Secondary

    All lanes: Goat anti-rabbit IgG at 1/50000 dilution

    Predicted band size: 39 kDa

    Observed band size: 39 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HAO2 antibody (ab229817), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HAO2 antibody (ab229817)

    Paraffin-embedded human kidney tissue stained for HAO2 using ab229817 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HAO2 antibody (ab229817), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HAO2 antibody (ab229817)

    Paraffin-embedded human liver tissue stained for HAO2 using ab229817 at 1/100 dilution in immunohistochemical analysis.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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