Rabbit Polyclonal HAO2 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Hydroxyacid oxidase 2 aa 1-200.
IgG
Rabbit
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
WB | IHC-P | |
---|---|---|
Human | Tested | Tested |
Mouse | Tested | Expected |
Rat | Tested | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500 - 1/5000 | Notes - |
Species Mouse | Dilution info 1/500 - 1/5000 | Notes - |
Species Rat | Dilution info 1/500 - 1/5000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/20 - 1/200 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Oxidase that catalyzes the oxidation of medium and long chain hydroxyacids such as 2-hydroxyhexadecanoate and 2-hydroxyoctanoate, to the correspondong 2-oxoacids (PubMed:10777549). Its role in the oxidation of 2-hydroxy fatty acids may contribute to the general pathway of fatty acid alpha-oxidation (Probable). Active in vitro with the artificial electron acceptor 2,6-dichlorophenolindophenol (DCIP), but O2 is believed to be the physiological electron acceptor, leading to the production of H2O2. Is not active on glycolate, glyoxylate, L-lactate and 2-hydroxybutanoate (PubMed:10777549).
HAOX2, GIG16, HAO2, HAOX2, GIG16, 2-Hydroxyacid oxidase 2, HAOX2, Cell growth-inhibiting gene 16 protein, Long chain alpha-hydroxy acid oxidase, Long-chain L-2-hydroxy acid oxidase
Rabbit Polyclonal HAO2 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Hydroxyacid oxidase 2 aa 1-200.
IgG
Rabbit
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
Affinity purification Protein G
Purity >95%.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
The HAO2 protein also known as hydroxyacid oxidase 2 plays an enzyme role in the cellular environment. It catalyzes the oxidation of glycolate to glyoxylate which involves the substrate binding and conversion. With a molecular mass approximately 40 kDa HAO2 expresses mainly in tissues like liver and pancreas where it supports metabolic processes. Understanding its precise mechanical role helps clarify its action in metabolic pathways.
HAO2 functions as a significant part of the peroxisomal beta-oxidation process. It does not seem to act as a component of a larger protein complex; it rather operates independently in modifying substrates. Its actions contribute to the regulation of cellular pH and reactive oxygen species through its enzymatic reactions. HAO2’s activity impacts cellular energy balance by participating in specific metabolic processes involving medium-chain hydroxy acids.
HAO2 integrates into the metabolic pathway of glyoxylate and dicarboxylate metabolism where it executes important reactions involving hydroxy acids. In addition HAO2 co-functions with proteins such as lactate dehydrogenase in the related energy metabolism pathways aiding in energy production and balance within cells. These interconnected pathways exhibit how HAO2 supports metabolic function and stability.
HAO2 shows relevance in metabolic disorders such as primary hyperoxaluria. This condition results from an overproduction of oxalate due to defective substrate metabolism where HAO2 acts. Furthermore aberrant HAO2 function associates with renal issues due to its role in oxalate metabolism. In this context HAO2 has an indirect connection to proteins like alanine-glyoxylate aminotransferase which also impacts oxalate metabolism linking them through the biological disorder framework.
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All lanes: Western blot - Anti-HAO2 antibody (ab229817) at 1/500 dilution
Lane 1: MCF7 (human breast adenocarcinoma cell line) lysate
Lane 2: Rat kidney lysate
Lane 3: Mouse kidney lysate
All lanes: Goat anti-rabbit IgG at 1/50000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDa
Paraffin-embedded human kidney tissue stained for HAO2 using ab229817 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human liver tissue stained for HAO2 using ab229817 at 1/100 dilution in immunohistochemical analysis.
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