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AB181997

Anti-HAPLN1 antibody [EPR6338]

3

(3 Reviews)

|

(4 Publications)

Rabbit Recombinant Monoclonal HAPLN1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 4 publications.

View Alternative Names

CRTL1, HAPLN1, Hyaluronan and proteoglycan link protein 1, Cartilage-linking protein 1, Proteoglycan link protein, Cartilage-link protein

5 Images
Flow Cytometry (Intracellular) - Anti-HAPLN1 antibody [EPR6338] (AB181997)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-HAPLN1 antibody [EPR6338] (AB181997)

Intracellular Flow Cytometry analysis of MCF-7 (human breast carcinoma) cells labeling HAPLN1 with purified ab181997 at 1/2000 dilution (red). The secondary antibody was Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution. A Rabbit monoclonal IgG (Black) was used as the isotype control and cells without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.

Immunocytochemistry/ Immunofluorescence - Anti-HAPLN1 antibody [EPR6338] (AB181997)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HAPLN1 antibody [EPR6338] (AB181997)

Immunofluorescent analysis of MCF7 cells (paraformaldehyde-fixed, 4%) labeling HAPLN1 with ab181997 at 1/250 dilution, followed by Goat anti rabbit IgG(Dylight 488) at 1/200 dilution and counter-stained with DAPI (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HAPLN1 antibody [EPR6338] (AB181997)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HAPLN1 antibody [EPR6338] (AB181997)

Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling HAPLN1 with ab181997 at 1/50 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Western blot - Anti-HAPLN1 antibody [EPR6338] (AB181997)
  • WB

Supplier Data

Western blot - Anti-HAPLN1 antibody [EPR6338] (AB181997)

All lanes:

Western blot - Anti-HAPLN1 antibody [EPR6338] (ab181997) at 1/1000 dilution

All lanes:

Human spleen lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/1000 dilution

Predicted band size: 40 kDa

false

Western blot - Anti-HAPLN1 antibody [EPR6338] (AB181997)
  • WB

Supplier Data

Western blot - Anti-HAPLN1 antibody [EPR6338] (AB181997)

All lanes:

Western blot - Anti-HAPLN1 antibody [EPR6338] (ab181997) at 1/1000 dilution

Lane 1:

MCF7 lysate at 10 µg

Lane 2:

Caco2 lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L)-HRP at 1/1000 dilution

Predicted band size: 40 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6338

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, Flow Cyt (Intra), WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/2000", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HAPLN1 also known as hyaluronan and proteoglycan link protein 1 is an important structural component in the extracellular matrix. This protein links hyaluronan to proteoglycans which contributes to the integrity and function of the matrix. HAPLN1 has a molecular mass of approximately 39 kDa and is expressed in various tissues including cartilage and nervous tissues. Its expression plays a significant role in maintaining the properties of these tissues.
Biological function summary

Hyaluronan and proteoglycan link protein 1 supports the organization and stabilization of the extracellular matrix network. As part of this framework HAPLN1 forms complexes with hyaluronan and aggrecan ensuring structural stability and resilience in tissues experiencing mechanical stress. The association with such complexes impacts cellular signaling and tissue hydration which are important for maintaining tissue homeostasis and function.

Pathways

HAPLN1 participates in extracellular matrix organization and cell signaling processes. It is actively involved in the aggrecan pathway which influences cartilage development and repair. HAPLN1 is also connected to proteins like aggrecan and link proteins that help mediate interactions within the matrix and regulate cellular responses to mechanical stress.

Abnormal expression or function of HAPLN1 has links to osteoarthritis and intervertebral disc degeneration. In these conditions the disruption of extracellular matrix composition alters tissue integrity and function. An imbalance involving HAPLN1 along with proteins like aggrecan contributes to the pathogenesis and progression of these disorders affecting joint and disc health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Stabilizes the aggregates of proteoglycan monomers with hyaluronic acid in the extracellular cartilage matrix.
See full target information HAPLN1
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of pharmaceutical analysis 14:335-347 PubMed38618242

2024

Hapln1 promotes dedifferentiation and proliferation of iPSC-derived cardiomyocytes by promoting versican-based GDF11 trapping.

Applications

Unspecified application

Species

Unspecified reactive species

Ding-Jun Hao,Yue Qin,Shi-Jie Zhou,Bu-Huai Dong,Jun-Song Yang,Peng Zou,Li-Ping Wang,Yuan-Ting Zhao

Journal of cellular physiology 237:2469-2477 PubMed35220586

2022

Single-cell RNA sequencing reveals the cell types heterogenicity of human discoid lateral meniscus cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Sun,Wenhui Zhang,Fangzhou Liu,Deng Li,Zhiqing Cai,Zhencheng Huang,Meiyi Chen,Zhencan Lin,Jie Xu,Ruofan Ma

Molecular psychiatry 23:2302-2313 PubMed30254235

2018

The hippocampal extracellular matrix regulates pain and memory after injury.

Applications

Unspecified application

Species

Unspecified reactive species

Maral Tajerian,Victor Hung,Huy Nguyen,Gail Lee,Lydia-Marie Joubert,Andrey Victorovich Malkovskiy,Bende Zou,Simon Xie,Ting-Ting Huang,J David Clark

Neuron 99:702-719.e6 PubMed30078576

2018

Extracellular Matrix Components HAPLN1, Lumican, and Collagen I Cause Hyaluronic Acid-Dependent Folding of the Developing Human Neocortex.

Applications

Unspecified application

Species

Unspecified reactive species

Katherine R Long,Ben Newland,Marta Florio,Nereo Kalebic,Barbara Langen,Anna Kolterer,Pauline Wimberger,Wieland B Huttner
View all publications
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