Rabbit Recombinant Monoclonal HAPLN1 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples.
Images
![Flow Cytometry (Intracellular) - Anti-HAPLN1 antibody [EPR6338] - BSA and Azide free (AB240257), expandable thumbnail](https://content.abcam.com/products/images/hapln1-antibody-epr6338-bsa-and-azide-free-ab240257--flow-cytometry-intracellular-img136695.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-HAPLN1 antibody [EPR6338] - BSA and Azide free (AB240257)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|
| Human | Expected | Tested | Tested | Tested |
| Mouse | Expected | Predicted | Predicted | Predicted |
| Rat | Expected | Predicted | Predicted | Predicted |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Stabilizes the aggregates of proteoglycan monomers with hyaluronic acid in the extracellular cartilage matrix.
Alternative names
CRTL1, HAPLN1, Hyaluronan and proteoglycan link protein 1, Cartilage-linking protein 1, Proteoglycan link protein, Cartilage-link protein
Recommended products
Rabbit Recombinant Monoclonal HAPLN1 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR6338
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab240257 is the carrier-free version of Anti-HAPLN1 antibody [EPR6338] ab181997.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
HAPLN1 also known as hyaluronan and proteoglycan link protein 1 is an important structural component in the extracellular matrix. This protein links hyaluronan to proteoglycans which contributes to the integrity and function of the matrix. HAPLN1 has a molecular mass of approximately 39 kDa and is expressed in various tissues including cartilage and nervous tissues. Its expression plays a significant role in maintaining the properties of these tissues.
- Biological function summary
Hyaluronan and proteoglycan link protein 1 supports the organization and stabilization of the extracellular matrix network. As part of this framework HAPLN1 forms complexes with hyaluronan and aggrecan ensuring structural stability and resilience in tissues experiencing mechanical stress. The association with such complexes impacts cellular signaling and tissue hydration which are important for maintaining tissue homeostasis and function.
- Pathways
HAPLN1 participates in extracellular matrix organization and cell signaling processes. It is actively involved in the aggrecan pathway which influences cartilage development and repair. HAPLN1 is also connected to proteins like aggrecan and link proteins that help mediate interactions within the matrix and regulate cellular responses to mechanical stress.
- Associated diseases and disorders
Abnormal expression or function of HAPLN1 has links to osteoarthritis and intervertebral disc degeneration. In these conditions the disruption of extracellular matrix composition alters tissue integrity and function. An imbalance involving HAPLN1 along with proteins like aggrecan contributes to the pathogenesis and progression of these disorders affecting joint and disc health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Flow Cytometry (Intracellular) - Anti-HAPLN1 antibody [EPR6338] - BSA and Azide free (ab240257), expandable thumbnail](https://content.abcam.com/products/images/hapln1-antibody-epr6338-bsa-and-azide-free-ab240257--flow-cytometry-intracellular-img136695.jpg "Flow Cytometry (Intracellular) - Anti-HAPLN1 antibody [EPR6338] - BSA and Azide free (ab240257)")
Flow Cytometry (Intracellular) - Anti-HAPLN1 antibody [EPR6338] - BSA and Azide free (ab240257)
Intracellular Flow Cytometry analysis of MCF-7 (human breast carcinoma) cells labeling HAPLN1 with purified Anti-HAPLN1 antibody [EPR6338] ab181997 at 1/2000 dilution (red). The secondary antibody was Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution. A Rabbit monoclonal IgG (Black) was used as the isotype control and cells without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-HAPLN1 antibody [EPR6338] ab181997).
![Immunocytochemistry/ Immunofluorescence - Anti-HAPLN1 antibody [EPR6338] - BSA and Azide free (ab240257), expandable thumbnail](https://content.abcam.com/products/images/hapln1-antibody-epr6338-bsa-and-azide-free-ab240257--immunocytochemistry-immunofluorescence-img63161.gif "Immunocytochemistry/ Immunofluorescence - Anti-HAPLN1 antibody [EPR6338] - BSA and Azide free (ab240257)")
Immunocytochemistry/ Immunofluorescence - Anti-HAPLN1 antibody [EPR6338] - BSA and Azide free (ab240257)
Immunofluorescent analysis of MCF7 cells (paraformaldehyde-fixed, 4%) labeling HAPLN1 with Anti-HAPLN1 antibody [EPR6338] ab181997 at 1/250 dilution, followed by Goat anti rabbit IgG(Dylight 488) at 1/200 dilution and counter-stained with DAPI (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-HAPLN1 antibody [EPR6338] ab181997).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HAPLN1 antibody [EPR6338] - BSA and Azide free (ab240257), expandable thumbnail](https://content.abcam.com/products/images/hapln1-antibody-epr6338-bsa-and-azide-free-ab240257--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136931.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HAPLN1 antibody [EPR6338] - BSA and Azide free (ab240257)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HAPLN1 antibody [EPR6338] - BSA and Azide free (ab240257)
Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling HAPLN1 with Anti-HAPLN1 antibody [EPR6338] ab181997 at 1/50 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-HAPLN1 antibody [EPR6338] ab181997).
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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