Anti-Haptoglobin antibody [EPR22856-212]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Haptoglobin antibody [EPR22856-212] (AB256454)

Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Haptoglobin with ab256454 at 1/5000 dilution (0.149 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in rat liver. The section was incubated with ab256454 for 10 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

• IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Haptoglobin antibody [EPR22856-212] (AB256454)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen section of rat lung tissue labeling Haptoglobin with ab256454 at 1/500 dilution, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution (green). The nuclear counter stain is DAPI (blue). Positive staining on alveolar epithelial type II cells of rat lung (PMID : 19501987).

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Haptoglobin antibody [EPR22856-212] (AB256454)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Hepa1-6 (mouse hepatoma epithelial cell) cells labeling Haptoglobin with ab256454 at 1/700 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Haptoglobin antibody [EPR22856-212] (AB256454)

Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Haptoglobin with ab256454 at 1/5000 dilution (0.149 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in mouse lung (PMID : 14697247). The section was incubated with ab256454 for 10 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

• IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Haptoglobin antibody [EPR22856-212] (AB256454)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen section of mouse lung tissue labeling Haptoglobin with ab256454 at 1/500 dilution, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution (green). The nuclear counter stain is DAPI (blue). Positive staining on alveolar epithelial type II cells and bronchi of mouse lung (PMID : 19501987).

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Haptoglobin antibody [EPR22856-212] (AB256454)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Haptoglobin with ab256454 at 1/5000 dilution (0.149 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in mouse liver (PMID : 14697247). The section was incubated with ab256454 for 10 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

• WB

Lab

Western blot - Anti-Haptoglobin antibody [EPR22856-212] (AB256454)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 19805376, 16543473).

Exposure time : Lanes 1-2 : 3 seconds; Lane 3 : 10 seconds.

All lanes:

Western blot - Anti-Haptoglobin antibody [EPR22856-212] (ab256454) at 1/1000 dilution

Lane 1:

Human serum at 20 µg

Lane 2:

Human plasma at 20 µg

Lane 3:

Human liver lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 45 kDa

Observed band size: 42 kDa,45 kDa

false

• WB

Lab

Western blot - Anti-Haptoglobin antibody [EPR22856-212] (AB256454)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 19805376, 16543473).

Exposure time : Lanes 1-2 : 3 6 seconds; Lanes 3-4 : 3minutes; Lane 5 : 3 seconds; Lane 6 : 59 seconds.

All lanes:

Western blot - Anti-Haptoglobin antibody [EPR22856-212] (ab256454) at 1/2000 dilution

Lane 1:

Mouse serum at 20 µg

Lane 2:

Mouse plasma at 20 µg

Lane 3:

Mouse liver lysate at 20 µg

Lane 4:

Mouse lung lysate at 20 µg

Lane 5:

Rat plasma at 20 µg

Lane 6:

Rat liver lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 45 kDa

Observed band size: 36 kDa,42 kDa,45 kDa,52 kDa

false