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Rabbit Recombinant Monoclonal Haptoglobin antibody. Suitable for IHC-P, WB, IHC-Fr, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 7 publications.

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Images

Western blot - Anti-Haptoglobin antibody [EPR22856-212] (AB256454), expandable thumbnail
  • Western blot - Anti-Haptoglobin antibody [EPR22856-212] (AB256454), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Haptoglobin antibody [EPR22856-212] (AB256454), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Haptoglobin antibody [EPR22856-212] (AB256454), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Haptoglobin antibody [EPR22856-212] (AB256454), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IFIHC-FrFlow Cyt (Intra)
Human
Expected
Not recommended
Tested
Not recommended
Expected
Expected
Mouse
Tested
Not recommended
Tested
Not recommended
Tested
Tested
Rat
Tested
Not recommended
Tested
Not recommended
Tested
Expected

Tested
Tested

Species
Mouse
Dilution info
1/5000
Notes

This antibody is not recommended for human IHC-P.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/5000
Notes

This antibody is not recommended for human IHC-P.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

-

Species
Rat
Dilution info
1/500
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/700
Notes

-

Expected
Expected

Species
Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an antioxidant, has antibacterial activity, and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidly cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway. The uncleaved form of allele alpha-2 (2-2), known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens.

Alternative names

Haptoglobin, Zonulin, HP

Recommended products

Rabbit Recombinant Monoclonal Haptoglobin antibody. Suitable for IHC-P, WB, IHC-Fr, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 7 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR22856-212
Purification technique
Affinity purification Protein A
Specificity

This antibody is not recommended for human IHC-P.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Haptoglobin (Hp) also known as Hp protein is a glycoprotein with a molecular weight ranging from 85 to 100 kDa varying due to its ability to form different dimeric and multimeric structures. It is primarily synthesized in the liver and circulating in the plasma. Mechanically haptoglobin binds free hemoglobin released from erythrocytes preventing oxidative damage and hemoglobin-driven deleterious effects. Haptoglobin's binding to hemoglobin forms a haptoglobin-hemoglobin complex that gets cleared by the CD163 receptor on macrophages mainly in the liver and spleen.

Biological function summary

Haptoglobin functions as an antioxidant and an acute phase protein playing roles in inflammation and immune response. It is an essential component of the antioxidant defense system reducing oxidative stress by binding free hemoglobin. Additionally haptoglobin can participate in complex formation with hemoglobin to facilitate the clearance of excess free hemoglobin from circulation. Its expression increases during inflammation under the regulation of cytokines like IL-6.

Pathways

The haptoglobin protein is part of the hemoglobin clearance pathway integral for iron metabolism and homeostasis. This pathway ensures the safe removal of hemoglobin from the blood helping to prevent kidney damage. Haptoglobin is related to other proteins such as CD163 and the liver-derived hepatocellular proteins involved in the clearance process. Also haptoglobin is linked to antioxidant pathways collaborating with oxidative stress regulators to manage reactive oxygen species levels.

Associated diseases and disorders

Changes in haptoglobin levels are associated with hemolytic anemia and inflammatory conditions. Low haptoglobin levels often suggest hemolytic anemia due to its rapid binding with free hemoglobin in the bloodstream. During inflammation and infections 'anti-Hp' tests (haptoglobin tests) may show increased haptoglobin levels as a response to cytokine signaling. Moreover haptoglobin polymorphisms have a connection with ischemic stroke susceptibility where its isoforms show differing efficiencies in binding free hemoglobin. These findings highlight haptoglobin's relevance as a biomarker for these conditions and illustrate its interaction with hematologic and immune proteins in disease contexts.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-Haptoglobin antibody [EPR22856-212] (ab256454), expandable thumbnail

    Western blot - Anti-Haptoglobin antibody [EPR22856-212] (ab256454)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID:19805376, 16543473).

    Exposure time: Lanes 1-2: 3 seconds; Lane 3: 10 seconds.

    All lanes: Western blot - Anti-Haptoglobin antibody [EPR22856-212] (ab256454) at 1/1000 dilution

    Lane 1: Human serum at 20 µg

    Lane 2: Human plasma at 20 µg

    Lane 3: Human liver lysate at 20 µg

    Secondary

    All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Predicted band size: 45 kDa

    Observed band size: 42 kDa, 45 kDa

  • Western blot - Anti-Haptoglobin antibody [EPR22856-212] (ab256454), expandable thumbnail

    Western blot - Anti-Haptoglobin antibody [EPR22856-212] (ab256454)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID:19805376, 16543473).

    Exposure time: Lanes 1-2:3 6 seconds; Lanes 3-4: 3minutes; Lane 5: 3 seconds; Lane 6: 59 seconds.

    All lanes: Western blot - Anti-Haptoglobin antibody [EPR22856-212] (ab256454) at 1/2000 dilution

    Lane 1: Mouse serum at 20 µg

    Lane 2: Mouse plasma at 20 µg

    Lane 3: Mouse liver lysate at 20 µg

    Lane 4: Mouse lung lysate at 20 µg

    Lane 5: Rat plasma at 20 µg

    Lane 6: Rat liver lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 45 kDa

    Observed band size: 36 kDa, 42 kDa, 45 kDa, 52 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Haptoglobin antibody [EPR22856-212] (ab256454), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Haptoglobin antibody [EPR22856-212] (ab256454)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Haptoglobin with ab256454 at 1/5000 dilution (0.149 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining in rat liver. The section was incubated with ab256454 for 10 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Haptoglobin antibody [EPR22856-212] (ab256454), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Haptoglobin antibody [EPR22856-212] (ab256454)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Haptoglobin with ab256454 at 1/5000 dilution (0.149 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining in mouse liver (PMID: 14697247). The section was incubated with ab256454 for 10 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Haptoglobin antibody [EPR22856-212] (ab256454), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Haptoglobin antibody [EPR22856-212] (ab256454)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Haptoglobin with ab256454 at 1/5000 dilution (0.149 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining in mouse lung (PMID: 14697247). The section was incubated with ab256454 for 10 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Flow Cytometry (Intracellular) - Anti-Haptoglobin antibody [EPR22856-212] (ab256454), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Haptoglobin antibody [EPR22856-212] (ab256454)

    Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Hepa1-6 (mouse hepatoma epithelial cell) cells labeling Haptoglobin with ab256454 at 1/700 dilution (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunohistochemistry (Frozen sections) - Anti-Haptoglobin antibody [EPR22856-212] (ab256454), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Haptoglobin antibody [EPR22856-212] (ab256454)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen section of rat lung tissue labeling Haptoglobin with ab256454 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (green). The nuclear counter stain is DAPI (blue). Positive staining on alveolar epithelial type II cells of rat lung (PMID: 19501987).

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Frozen sections) - Anti-Haptoglobin antibody [EPR22856-212] (ab256454), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Haptoglobin antibody [EPR22856-212] (ab256454)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen section of mouse lung tissue labeling Haptoglobin with ab256454 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (green). The nuclear counter stain is DAPI (blue). Positive staining on alveolar epithelial type II cells and bronchi of mouse lung (PMID: 19501987).

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

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Product protocols

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