Anti-Haptoglobin antibody [EPR22856-233]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Haptoglobin antibody [EPR22856-233] (AB256455)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Haptoglobin with ab256455 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in mouse liver (PMID : 14697247). The section was incubated with ab256455 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

• IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Haptoglobin antibody [EPR22856-233] (AB256455)

Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen mouse lung tissue labeling Haptoglobin with ab256455 at 1/500 (1.17 μg/ml) dilution followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Haptoglobin antibody [EPR22856-233] (AB256455)

Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue labeling Haptoglobin with ab256455 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control : no staining on mouse cerebral cortex (PMID : 14697247). The section was incubated with ab256455 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Haptoglobin antibody [EPR22856-233] (AB256455)

Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Haptoglobin with ab256455 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on bronchi of mouse lung (PMID : 14697247). The section was incubated with ab256455 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

• WB

Lab

Western blot - Anti-Haptoglobin antibody [EPR22856-233] (AB256455)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 19805376, 16543473).

Exposure time : Lanes 1-2 : 3 seconds; Lanes 3-4 : 103 seconds.

All lanes:

Western blot - Anti-Haptoglobin antibody [EPR22856-233] (ab256455) at 1/1000 dilution

Lane 1:

Mouse serum at 20 µg

Lane 2:

Mouse plasma at 20 µg

Lane 3:

Mouse liver lysate at 20 µg

Lane 4:

Mouse lung lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 45 kDa

Observed band size: 36 kDa,42 kDa,45 kDa,52 kDa

false