AB13429

Anti-Haptoglobin antibody [HG-36]

Name: Anti-Haptoglobin antibody [HG-36] (ab13429) | Abcam
Brand: Abcam Limited
SKU: AB13429
Price: 470 USD
Availability: InStock
Rating: 5 (10 reviews)

(10 Reviews)

(20 Publications)

Mouse Monoclonal Haptoglobin antibody. Suitable for I-ELISA, ELISA, Dot, WB, IHC-P, ICC/IF and reacts with Purified native protein - Human, Human samples. Cited in 20 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human HP.

View Alternative Names

Haptoglobin, Zonulin, HP

1 Images

Indirect ELISA - Anti-Haptoglobin antibody [HG-36] (AB13429)

I-ELISA

Supplier Data

Indirect ELISA - Anti-Haptoglobin antibody [HG-36] (AB13429)

ab13429 was serially diluted to detect Haptoglobin by indirect ELISA. Reactivity is observed with only haptoglobin but not with human IgG and a-1-acid-glycoprotein.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

HG-36

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC/IF, I-ELISA, ELISA, Dot

applications

Immunogen

Native Full Length Protein corresponding to Human HP.

P00738

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Reactivity data

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Product details

This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Purification technique

Proprietary technique

Purification notes

Purified from hybridoma cell culture

Storage buffer

pH: 7.4 Preservative: 0.098% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Haptoglobin (Hp) also known as Hp protein is a glycoprotein with a molecular weight ranging from 85 to 100 kDa varying due to its ability to form different dimeric and multimeric structures. It is primarily synthesized in the liver and circulating in the plasma. Mechanically haptoglobin binds free hemoglobin released from erythrocytes preventing oxidative damage and hemoglobin-driven deleterious effects. Haptoglobin's binding to hemoglobin forms a haptoglobin-hemoglobin complex that gets cleared by the CD163 receptor on macrophages mainly in the liver and spleen.

Biological function summary

Haptoglobin functions as an antioxidant and an acute phase protein playing roles in inflammation and immune response. It is an essential component of the antioxidant defense system reducing oxidative stress by binding free hemoglobin. Additionally haptoglobin can participate in complex formation with hemoglobin to facilitate the clearance of excess free hemoglobin from circulation. Its expression increases during inflammation under the regulation of cytokines like IL-6.

Pathways

The haptoglobin protein is part of the hemoglobin clearance pathway integral for iron metabolism and homeostasis. This pathway ensures the safe removal of hemoglobin from the blood helping to prevent kidney damage. Haptoglobin is related to other proteins such as CD163 and the liver-derived hepatocellular proteins involved in the clearance process. Also haptoglobin is linked to antioxidant pathways collaborating with oxidative stress regulators to manage reactive oxygen species levels.

Changes in haptoglobin levels are associated with hemolytic anemia and inflammatory conditions. Low haptoglobin levels often suggest hemolytic anemia due to its rapid binding with free hemoglobin in the bloodstream. During inflammation and infections 'anti-Hp' tests (haptoglobin tests) may show increased haptoglobin levels as a response to cytokine signaling. Moreover haptoglobin polymorphisms have a connection with ischemic stroke susceptibility where its isoforms show differing efficiencies in binding free hemoglobin. These findings highlight haptoglobin's relevance as a biomarker for these conditions and illustrate its interaction with hematologic and immune proteins in disease contexts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an antioxidant, has antibacterial activity, and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidly cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.. The uncleaved form of allele alpha-2 (2-2), known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens.

See full target information HP

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Publications (20)

Recent publications for all applications. Explore the full list and refine your search

Life (Basel, Switzerland) 15: PubMed40430166

2025

Immunohistochemical Expression of Haptoglobin in Skin Lesions of Hidradenitis Suppurativa.

Applications

Unspecified application

Species

Unspecified reactive species

Nessr Abu Rached,Hanna Telkemeyer,Marina Skrygan,Martin Doerler,Yannik Haven,Lennart Ocker,Daniel Myszkowski,Thomas Meyer,Markus Stücker,Eggert Stockfleth,Falk G Bechara

Heliyon 8:e11271 PubMed36387451

2022

First-in-human autologous implantation of genetically modified adipocytes expressing LCAT for the treatment of familial LCAT deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Masayuki Aso,Tokuo T Yamamoto,Masayuki Kuroda,Jun Wada,Yoshitaka Kubota,Ko Ishikawa,Yoshiro Maezawa,Naoya Teramoto,Ayako Tawada,Sakiyo Asada,Yasuyuki Aoyagi,Mika Kirinashizawa,Akinobu Onitake,Yuta Matsuura,Kunio Yasunaga,Shun-Ichi Konno,Katsuaki Nishino,Misato Yamamoto,Junko Miyoshi,Norihiko Kobayashi,Masami Tanio,Takayuki Ikeuchi,Hidetoshi Igari,Nobuyuki Mitsukawa,Hideki Hanaoka,Koutaro Yokote,Yasushi Saito

Liver international : official journal of the International Association for the Study of the Liver 41:2427-2432 PubMed34358398

2021

Hypertransaminasemia and liver fibrosis associated with haptoglobin retention and anhaptoglobinemia in a paediatric patient.

Applications

Unspecified application

Species

Unspecified reactive species

Sophia Gunzer,Andreas Kraus,Inka Buchroth,Marianne Grüneberg,Cordula Westermann,Saskia Biskup,Janine Reunert,Inga Grünewald,Thorsten Marquardt

Journal of personalized medicine 10: PubMed33096722

2020

Why Does COVID-19 Affect Patients with Spinal Cord Injury Milder? A Case-Control Study: Results from Two Observational Cohorts.

Applications

Unspecified application

Species

Unspecified reactive species

Enrique Calvo,Nerea Corbacho-Alonso,Tamara Sastre-Oliva,Estefania Nuñez,Patricia Baena-Galan,German Hernandez-Fernandez,Miguel Rodriguez-Cola,Irena Jimenez-Velasco,Fernando J Corrales,Claudia Gambarrutta-Malfati,Francisco Gutierrez-Henares,Elisa Lopez-Dolado,Angel Gil-Agudo,Jesus Vazquez,Laura Mourino-Alvarez,Maria G Barderas

Experimental and therapeutic medicine 19:741-747 PubMed31897108

2020

Identification of an activation-related protein in B cells in the ABO incompatible condition.

Applications

Unspecified application

Species

Unspecified reactive species

Jingsong Cao,Cong Chen,Luogen Liu,Yunsheng Zhang,Hong Zhou,Jianhua Xiao,Yi Wang

Proteomics. Clinical applications 14:e1900062 PubMed31532894

2019

Serum Small Extracellular Vesicles Proteome of Tuberculosis Patients Demonstrated Deregulated Immune Response.

Applications

Unspecified application

Species

Unspecified reactive species

Rakesh Arya,Deepti Dabral,Hossain Md Faruquee,Himangshu Mazumdar,Saurav Jyoti Patgiri,Trinayan Deka,Rumi Basumatary,Rukuwe-U Kupa,Chayale Semy,Wetetsho Kapfo,Kevideme Liegise,Inderjeet Kaur,Tenzin Choedon,Purnima Kumar,Rajendra Kumar Behera,Pranjal Deori,Reema Nath,Kerekha Khalo,Lahari Saikia,Vinotsole Khamo,Ranjan Kumar Nanda

Plastic and reconstructive surgery. Global open 7:e2339 PubMed31592376

2019

Skin Protein Profile after Major Weight Loss and Its Role in Body Contouring Surgery.

Applications

Unspecified application

Species

Unspecified reactive species

José Renato B Gallo,Larissa B Maschio-Signorini,Celso R B Cabral,Debora A P de Campos Zuccari,Maurício L Nogueira,Antônio R Bozola,Patricia M Cury,Alessandra Vidotto

Journal of Cancer 10:4165-4177 PubMed31413735

2019

iTRAQ-based Comparative Serum Proteomic Analysis of Prostate Cancer Patients with or without Bone Metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Yan,Binshen Chen,Shaoju Min,Yubo Gao,Yiming Zhang,Peng Xu,Chaoming Li,Jiasheng Chen,Guangheng Luo,Chunxiao Liu

Journal of proteome research 18:359-371 PubMed30370771

2018

Differential Quantitative Determination of Site-Specific Intact N-Glycopeptides in Serum Haptoglobin between Hepatocellular Carcinoma and Cirrhosis Using LC-EThcD-MS/MS.

Applications

Unspecified application

Species

Unspecified reactive species

Jianhui Zhu,Zhengwei Chen,Jie Zhang,Mingrui An,Jing Wu,Qing Yu,St John Skilton,Marshall Bern,K Ilker Sen,Lingjun Li,David M Lubman

Oncology letters 15:580-587 PubMed29391890

2018

Identification of proteins with the CDw75 epitope in human colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Óscar Mariño-Crespo,Almudena Fernández-Briera,Emilio Gil-Martín

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Haptoglobin antibody [HG-36]

Indirect ELISA - Anti-Haptoglobin antibody [HG-36] (AB13429)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (20)

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