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AB137591

Anti-HARS antibody

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(2 Publications)

Rabbit Polyclonal HARS antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HARS1 aa 1-300.

View Alternative Names

HARS, HRS, HARS1, Histidyl-tRNA synthetase, HisRS

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HARS antibody (AB137591)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HARS antibody (AB137591)

Immunohistochemical analysis of paraffin-embedded Human Hepatoma tissue, labelling HARS with ab137591 at 1/500 dilution.

Western blot - Anti-HARS antibody (AB137591)
  • WB

Unknown

Western blot - Anti-HARS antibody (AB137591)

7.5% SDS PAGE

All lanes:

Western blot - Anti-HARS antibody (ab137591) at 1/500 dilution

All lanes:

HCT116 whole cell lysate at 30 µg

Predicted band size: 57 kDa

false

Western blot - Anti-HARS antibody (AB137591)
  • WB

Unknown

Western blot - Anti-HARS antibody (AB137591)

7.5% SDS PAGE

All lanes:

Western blot - Anti-HARS antibody (ab137591) at 1/500 dilution

Lane 1:

HepG2 whole cell lysate at 30 µg

Lane 2:

HCT116 whole cell lysate at 30 µg

Predicted band size: 57 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human HARS1 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

P12081

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>or Tris-EDTA buffer (pH8.0)</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HARS also known as Histidyl-tRNA synthetase is an enzyme involved in the translation process of protein synthesis. It charges tRNA molecules with histidine facilitating the incorporation of this amino acid into growing polypeptide chains. HARS has an approximate molecular mass of 57 kDa. The enzyme is widely expressed in human tissues including high levels in the liver and skeletal muscle which supports its role in the active protein synthesis associated with these organs.
Biological function summary

Histidyl-tRNA synthetase plays a fundamental role in ensuring accuracy during protein synthesis by attaching histidine to its corresponding tRNA. This enzyme acts as a monomer but often interacts with other synthetases in a multisynthetase complex. By providing histidine-charged tRNAs HARS supports the translation machinery that synthesizes proteins based on encoded genetic instructions reflecting its essentiality in cellular health and function.

Pathways

HARS operates predominantly within the aminoacyl-tRNA biosynthesis pathway which is essential for protein translation. This pathway involves several other aminoacyl-tRNA synthetases including the leucyl-tRNA synthetase (LARS) and lysyl-tRNA synthetase (KARS) which facilitate the attachment of specific amino acids to their respective tRNAs. Additionally HARS links to the broader mTOR signaling pathway a critical pathway for regulating cell growth and protein synthesis in response to nutrient availability.

HARS mutations have association with a range of pathologies particularly autoimmune conditions such as myositis. Changes in HARS expression or function are also observed in neurodegenerative diseases like Charcot-Marie-Tooth disease a disorder characterized by peripheral nerve damage. Connections to these diseases highlight interactions between HARS and proteins involved in immune response and nerve function although the precise mechanisms may require further study.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the ATP-dependent ligation of histidine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (His-AMP) (PubMed : 29235198). Plays a role in axon guidance (PubMed : 26072516).
See full target information HARS1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in molecular biosciences 8:745990 PubMed34805271

2021

Hepatitis B Virus Promotes Hepatocellular Carcinoma Progression Synergistically With Hepatic Stellate Cells Facilitating the Expression and Secretion of ENPP2.

Applications

Unspecified application

Species

Unspecified reactive species

Wanyu Deng,Fu Chen,Ziyu Zhou,Yipei Huang,Junlong Lin,Fapeng Zhang,Gang Xiao,Chaoqun Liu,Chao Liu,Leibo Xu

Biochemistry 56:3619-3631 PubMed28632987

2017

The Usher Syndrome Type IIIB Histidyl-tRNA Synthetase Mutation Confers Temperature Sensitivity.

Applications

Unspecified application

Species

Unspecified reactive species

Jamie A Abbott,Ethan Guth,Cindy Kim,Cathy Regan,Victoria M Siu,C Anthony Rupar,Borries Demeler,Christopher S Francklyn,Susan M Robey-Bond
View all publications
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