AB249182

Anti-HARS antibody [EPR9451] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal HARS antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

HARS, HRS, HARS1, Histidyl-tRNA synthetase, HisRS

1 Images

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Western blot - Anti-HARS antibody [EPR9451] - BSA and Azide free (AB249182)

This data was developed using ab155087, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-HARS antibody [EPR9451] - BSA and Azide free (ab249182) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Lane 3:

Human fetal lung lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 57 kDa

false

Unconjugated

Anti-HARS antibody [EPR9451]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9451

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab249182 is the carrier-free version of ab155087.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HARS also known as Histidyl-tRNA synthetase is an enzyme involved in the translation process of protein synthesis. It charges tRNA molecules with histidine facilitating the incorporation of this amino acid into growing polypeptide chains. HARS has an approximate molecular mass of 57 kDa. The enzyme is widely expressed in human tissues including high levels in the liver and skeletal muscle which supports its role in the active protein synthesis associated with these organs.

Biological function summary

Histidyl-tRNA synthetase plays a fundamental role in ensuring accuracy during protein synthesis by attaching histidine to its corresponding tRNA. This enzyme acts as a monomer but often interacts with other synthetases in a multisynthetase complex. By providing histidine-charged tRNAs HARS supports the translation machinery that synthesizes proteins based on encoded genetic instructions reflecting its essentiality in cellular health and function.

Pathways

HARS operates predominantly within the aminoacyl-tRNA biosynthesis pathway which is essential for protein translation. This pathway involves several other aminoacyl-tRNA synthetases including the leucyl-tRNA synthetase (LARS) and lysyl-tRNA synthetase (KARS) which facilitate the attachment of specific amino acids to their respective tRNAs. Additionally HARS links to the broader mTOR signaling pathway a critical pathway for regulating cell growth and protein synthesis in response to nutrient availability.

HARS mutations have association with a range of pathologies particularly autoimmune conditions such as myositis. Changes in HARS expression or function are also observed in neurodegenerative diseases like Charcot-Marie-Tooth disease a disorder characterized by peripheral nerve damage. Connections to these diseases highlight interactions between HARS and proteins involved in immune response and nerve function although the precise mechanisms may require further study.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the ATP-dependent ligation of histidine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (His-AMP) (PubMed : 29235198). Plays a role in axon guidance (PubMed : 26072516).

See full target information HARS1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of clinical and experimental 13:2973-2983 PubMed33425098

2020

Adsorption of miR-218 by lncRNA HOTAIR regulates PDE7A and affects glioma cell proliferation, invasion, and apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yigong Wei,Kun Zhou,Cheng Wang,Xiaolin Du,Qing Xiao,Changyi Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HARS antibody [EPR9451] - BSA and Azide free

Western blot - Anti-HARS antibody [EPR9451] - BSA and Azide free (AB249182)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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