AB226222

Anti-Haspin antibody

Name: Anti-Haspin antibody (ab226222) | Abcam
Brand: Abcam Limited
SKU: AB226222
Price: 525 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(4 Publications)

Rabbit Polyclonal Haspin antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HASPIN aa 1-150.

View Alternative Names

GSG2, HASPIN, Serine/threonine-protein kinase haspin, Germ cell-specific gene 2 protein, H-haspin, Haploid germ cell-specific nuclear protein kinase

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-Haspin antibody (AB226222)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Haspin antibody (AB226222)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for Haspin (green) using ab226222 at 4 μg/ml in ICC/IF.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human HASPIN aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q8TF76

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Haspin also known as Germ Cell-Specific Gene 2 (GSG2) is a serine/threonine-protein kinase with a molecular weight of approximately 86 kDa. Haspin expresses predominantly in the testes but researchers have detected it in other tissues too. This kinase is well recognized for its role during cell division especially in the regulation of chromosome alignment and segregation by phosphorylating histone H3 at threonine 3.

Biological function summary

Haspin kinase plays a significant role in mitosis and meiosis. It orchestrates the proper attachment of microtubules to kinetochores by modifying chromosomal components. Haspin is part of a larger protein complex during cell division working closely with the chromosomal passenger complex to ensure accurate segregation of chromosomes. Its activity ensures the integrity and stability of cellular division processes.

Pathways

Haspin kinase functions centrally in cell cycle regulation and mitotic spindle assembly pathways. It interacts with key proteins in these pathways such as Aurora B kinase. The phosphorylation of histone H3 by haspin establishes a critical docking site for proteins in the chromosomal passenger complex facilitating proper chromosome alignment and spindle checkpoint activation.

Incorrect regulation of haspin activity associates with various cancers and chromosomal instability disorders. Overexpression or mutations in haspin can contribute to tumorigenesis by disrupting normal cell division. Additionally haspin's interaction with the chromosomal passenger complex proteins like survivin influences the development of colorectal cancer.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Serine/threonine-protein kinase that phosphorylates histone H3 at 'Thr-3' (H3T3ph) during mitosis. May act through H3T3ph to both position and modulate activation of AURKB and other components of the chromosomal passenger complex (CPC) at centromeres to ensure proper chromatid cohesion, metaphase alignment and normal progression through the cell cycle.

See full target information HASPIN

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:22679 PubMed40596516

2025

DNA probe pulldown screening uncovers O-GlcNAcylation modulation of transcription factor DNA interactions.

Applications

Unspecified application

Species

Unspecified reactive species

Guofang Li,Fanxu Meng,Xiaomin Zhong,Kairan Yu,Nana Zhang,Keren Zhang,Huang Huang,Wenli Li,Jianing Zhang,Wei Wang,Yan Ren,Yubo Liu

iScience 26:108011 PubMed37841592

2023

A Haspin-ARHGAP11A axis regulates epithelial morphogenesis through Rho-ROCK dependent modulation of LIMK1-Cofilin.

Applications

Unspecified application

Species

Unspecified reactive species

Roberto Quadri,Giuseppe Rotondo,Sarah Sertic,Sara Pozzi,Maria Chiara dell'Oca,Luisa Guerrini,Marco Muzi-Falconi

International journal of molecular sciences 22: PubMed34299370

2021

Phosphorylation of H3-Thr3 by Haspin Is Required for Primary Cilia Regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Roberto Quadri,Sarah Sertic,Anna Ghilardi,Diego Rondelli,Guido Roberto Gallo,Luca Del Giacco,Marco Muzi-Falconi

Cancer research 80:798-810 PubMed31882401

2019

Inhibition of Haspin Kinase Promotes Cell-Intrinsic and Extrinsic Antitumor Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Johannes C Melms,Sreeram Vallabhaneni,Caitlin E Mills,Clarence Yapp,Jia-Yun Chen,Eugenio Morelli,Patricia Waszyk,Sushil Kumar,Derrick Deming,Nienke Moret,Steven Rodriguez,Kartik Subramanian,Meri Rogava,Adam N R Cartwright,Adrienne Luoma,Shaolin Mei,Titus J Brinker,David M Miller,Alexander Spektor,Dirk Schadendorf,Nicolo Riggi,Kai W Wucherpfennig,Peter K Sorger,Benjamin Izar

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Haspin antibody

Immunocytochemistry/ Immunofluorescence - Anti-Haspin antibody (AB226222)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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