Rabbit Polyclonal Haspin antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HASPIN aa 1-150.
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- iScience 26:1080112023A Haspin-ARHGAP11A axis regulates epithelial morphogenesis through Rho-ROCK dependent modulation of LIMK1-Cofilin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRoberto Quadri,Giuseppe Rotondo,Sarah Sertic,Sara Pozzi,Maria Chiara dell'Oca,Luisa Guerrini,Marco Muzi-FalconiPubMed 37841592
- International journal of molecular sciences 22:2021Phosphorylation of H3-Thr3 by Haspin Is Required for Primary Cilia Regulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRoberto Quadri,Sarah Sertic,Anna Ghilardi,Diego Rondelli,Guido Roberto Gallo,Luca Del Giacco,Marco Muzi-FalconiPubMed 34299370
- Cancer research 80:798-8102019Inhibition of Haspin Kinase Promotes Cell-Intrinsic and Extrinsic Antitumor Activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJohannes C Melms,Sreeram Vallabhaneni,Caitlin E Mills,Clarence Yapp,Jia-Yun Chen,Eugenio Morelli,Patricia Waszyk,Sushil Kumar,Derrick Deming,Nienke Moret,Steven Rodriguez,Kartik Subramanian,Meri Rogava,Adam N R Cartwright,Adrienne Luoma,Shaolin Mei,Titus J Brinker,David M Miller,Alexander Spektor,Dirk Schadendorf,Nicolo Riggi,Kai W Wucherpfennig,Peter K Sorger,Benjamin IzarPubMed 31882401
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human HASPIN aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q8TF76
Reactivity data
Select an application| ICC/IF | |
|---|---|
| Human | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
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Target data
Function
Serine/threonine-protein kinase that phosphorylates histone H3 at 'Thr-3' (H3T3ph) during mitosis. May act through H3T3ph to both position and modulate activation of AURKB and other components of the chromosomal passenger complex (CPC) at centromeres to ensure proper chromatid cohesion, metaphase alignment and normal progression through the cell cycle.
Alternative names
Serine/threonine-protein kinase haspin, Germ cell-specific gene 2 protein, H-haspin, Haploid germ cell-specific nuclear protein kinase, HASPIN, GSG2
Recommended products
Rabbit Polyclonal Haspin antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HASPIN aa 1-150.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human HASPIN aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q8TF76
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Haspin also known as Germ Cell-Specific Gene 2 (GSG2) is a serine/threonine-protein kinase with a molecular weight of approximately 86 kDa. Haspin expresses predominantly in the testes but researchers have detected it in other tissues too. This kinase is well recognized for its role during cell division especially in the regulation of chromosome alignment and segregation by phosphorylating histone H3 at threonine 3.
- Biological function summary
Haspin kinase plays a significant role in mitosis and meiosis. It orchestrates the proper attachment of microtubules to kinetochores by modifying chromosomal components. Haspin is part of a larger protein complex during cell division working closely with the chromosomal passenger complex to ensure accurate segregation of chromosomes. Its activity ensures the integrity and stability of cellular division processes.
- Pathways
Haspin kinase functions centrally in cell cycle regulation and mitotic spindle assembly pathways. It interacts with key proteins in these pathways such as Aurora B kinase. The phosphorylation of histone H3 by haspin establishes a critical docking site for proteins in the chromosomal passenger complex facilitating proper chromosome alignment and spindle checkpoint activation.
- Associated diseases and disorders
Incorrect regulation of haspin activity associates with various cancers and chromosomal instability disorders. Overexpression or mutations in haspin can contribute to tumorigenesis by disrupting normal cell division. Additionally haspin's interaction with the chromosomal passenger complex proteins like survivin influences the development of colorectal cancer.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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We do not recommend this combination. It is not covered by our product promise.
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1 product image
Immunocytochemistry/ Immunofluorescence - Anti-Haspin antibody (ab226222)
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for Haspin (green) using ab226222 at 4 μg/ml in ICC/IF.
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