AB247763

Anti-HAUSP / USP7 antibody [EPR4254] - BSA and Azide free

RabMAb
Recombinant
KO Validated
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Rabbit Recombinant Monoclonal HAUSP / USP7 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

HAUSP, USP7, Ubiquitin carboxyl-terminal hydrolase 7, Deubiquitinating enzyme 7, Herpesvirus-associated ubiquitin-specific protease, Ubiquitin thioesterase 7, Ubiquitin-specific-processing protease 7

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Western blot - Anti-HAUSP / USP7 antibody [EPR4254] - BSA and Azide free (AB247763)

This data was developed using ab109109, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-HAUSP / USP7 antibody [EPR4254] (<a href='/en-us/products/primary-antibodies/hausp-usp7-antibody-epr4254-ab109109'>ab109109</a>) at 1/1000 dilution

Lane 1:

T47-D cell lysate at 10 µg

Lane 2:

Hela cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 128 kDa

Observed band size: 135 kDa

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Lab

Western blot - Anti-HAUSP / USP7 antibody [EPR4254] - BSA and Azide free (AB247763)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109109).

Lanes 1- 2 : Merged signal (red and green). Green - ab109109 observed at 128 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

ab109109 was shown to react with HAUSP / USP7 in wild-type HEK-293T cells in western blot. The band observed in CRISPR/Cas9 edited cell line ab266535 (CRISPR/Cas9 edited cell lysate ab257284) lane below 128kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HEK-293T and USP7 CRISPR/Cas9 edited HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab109109 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-HAUSP / USP7 antibody [EPR4254] (<a href='/en-us/products/primary-antibodies/hausp-usp7-antibody-epr4254-ab109109'>ab109109</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

USP7 CRISPR/Cas9 edited HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human USP7 (HAUSP) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-usp7-hausp-knockout-hek-293t-cell-line-ab266535'>ab266535</a>)

Predicted band size: 128 kDa

Observed band size: 128 kDa

false

Lab

Western blot - Anti-HAUSP / USP7 antibody [EPR4254] - BSA and Azide free (AB247763)

This data was developed using ab109109, the same antibody clone in a different buffer formulation.

Lane 1 : Wild-type HAP1 cell lysate (20 μg)

Lane 2 : HAUSP / USP7 knockout HAP1 cell lysate (20 μg)

Lane 3 : HeLa cell lysate (20 μg)

Lane 4 : MCF-7 cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab109109 observed at 135 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab109109 was shown to specifically react with HAUSP / USP7 when HAUSP / USP7 knockout samples were used. Wild-type and HAUSP / USP7 knockout samples were subjected to SDS-PAGE. ab109109 and ab8245 (loading control to GADPH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-HAUSP / USP7 antibody [EPR4254] (<a href='/en-us/products/primary-antibodies/hausp-usp7-antibody-epr4254-ab109109'>ab109109</a>)

Predicted band size: 128 kDa

false

Unconjugated

Anti-HAUSP / USP7 antibody [EPR4254]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4254

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab247763 is the carrier-free version of ab109109.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HAUSP also known as USP7 is a deubiquitinating enzyme with a molecular mass of approximately 135 kDa. It functions by removing ubiquitin molecules from target proteins influencing their stability and activity. HAUSP is widely expressed in various human tissues with notable presence in the nucleus and cytoplasm. Its expression is essential for modulation of multiple signaling pathways. The enzyme's capacity to regulate protein ubiquitination dynamics makes it a significant player in cellular function.

Biological function summary

HAUSP influences several critical cellular processes including DNA repair transcriptional regulation and cell cycle progression. It often interacts with other proteins such as p53 to modify their functions by altering their ubiquitination status. HAUSP does not function in isolation but forms part of larger protein complexes where it plays a role in processing substrate proteins. Its ability to activate or deactivate proteins through deubiquitination highlights its importance in maintaining cellular homeostasis.

Pathways

Research identifies HAUSP as an important component in both the p53 pathway and the Wnt signaling pathway. Through the p53 pathway HAUSP directly interacts with the p53 tumor suppressor protein influencing cell cycle and apoptosis decisions. Its participation in the Wnt signaling pathway associates it with beta-catenin where it supports cellular proliferation and differentiation. These pathway interactions illustrate HAUSP's integral role in maintaining cellular function and responding to various signaling inputs.

HAUSP has implications in cancer and neurodegenerative diseases. Aberrant activity or expression of HAUSP has been linked to tumorigenesis particularly through its interaction with the proteins p53 and MDM2 affecting cell survival and proliferation. In neurodegenerative conditions altered HAUSP activity may impact protein homeostasis and degradation pathways contributing to disease progression. Its connection to these proteins and disease states highlights its potential as a therapeutic target in associated disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Hydrolase that deubiquitinates target proteins such as ARMC5, FOXO4, DEPTOR, KAT5, p53/TP53, MDM2, ERCC6, DNMT1, UHRF1, PTEN, KMT2E/MLL5 and DAXX (PubMed : 11923872, PubMed : 15053880, PubMed : 16964248, PubMed : 18716620, PubMed : 25283148, PubMed : 25865756, PubMed : 26678539, PubMed : 28655758, PubMed : 33544460, PubMed : 35216969). Together with DAXX, prevents MDM2 self-ubiquitination and enhances the E3 ligase activity of MDM2 towards p53/TP53, thereby promoting p53/TP53 ubiquitination and proteasomal degradation (PubMed : 15053880, PubMed : 16845383, PubMed : 18566590, PubMed : 20153724). Deubiquitinates p53/TP53, preventing degradation of p53/TP53, and enhances p53/TP53-dependent transcription regulation, cell growth repression and apoptosis (PubMed : 25283148). Deubiquitinates p53/TP53 and MDM2 and strongly stabilizes p53/TP53 even in the presence of excess MDM2, and also induces p53/TP53-dependent cell growth repression and apoptosis (PubMed : 11923872, PubMed : 26786098). Deubiquitination of FOXO4 in presence of hydrogen peroxide is not dependent on p53/TP53 and inhibits FOXO4-induced transcriptional activity (PubMed : 16964248). In association with DAXX, is involved in the deubiquitination and translocation of PTEN from the nucleus to the cytoplasm, both processes that are counteracted by PML (PubMed : 18716620). Deubiquitinates KMT2E/MLL5 preventing KMT2E/MLL5 proteasomal-mediated degradation (PubMed : 26678539). Involved in cell proliferation during early embryonic development. Involved in transcription-coupled nucleotide excision repair (TC-NER) in response to UV damage : recruited to DNA damage sites following interaction with KIAA1530/UVSSA and promotes deubiquitination of ERCC6, preventing UV-induced degradation of ERCC6 (PubMed : 22466611, PubMed : 22466612). Involved in maintenance of DNA methylation via its interaction with UHRF1 and DNMT1 : acts by mediating deubiquitination of UHRF1 and DNMT1, preventing their degradation and promoting DNA methylation by DNMT1 (PubMed : 21745816, PubMed : 22411829). Deubiquitinates alkylation repair enzyme ALKBH3. OTUD4 recruits USP7 and USP9X to stabilize ALKBH3, thereby promoting the repair of alkylated DNA lesions (PubMed : 25944111). Acts as a chromatin regulator via its association with the Polycomb group (PcG) multiprotein PRC1-like complex; may act by deubiquitinating components of the PRC1-like complex (PubMed : 20601937). Able to mediate deubiquitination of histone H2B; it is however unsure whether this activity takes place in vivo (PubMed : 20601937). Exhibits a preference towards 'Lys-48'-linked ubiquitin chains (PubMed : 22689415). Increases regulatory T-cells (Treg) suppressive capacity by deubiquitinating and stabilizing the transcription factor FOXP3 which is crucial for Treg cell function (PubMed : 23973222). Plays a role in the maintenance of the circadian clock periodicity via deubiquitination and stabilization of the CRY1 and CRY2 proteins (PubMed : 27123980). Deubiquitinates REST, thereby stabilizing REST and promoting the maintenance of neural progenitor cells (PubMed : 21258371). Deubiquitinates SIRT7, inhibiting SIRT7 histone deacetylase activity and regulating gluconeogenesis (PubMed : 28655758). Involved in the regulation of WASH-dependent actin polymerization at the surface of endosomes and the regulation of endosomal protein recycling (PubMed : 26365382). It maintains optimal WASH complex activity and precise F-actin levels via deubiquitination of TRIM27 and WASHC1 (PubMed : 26365382). Mediates the deubiquitination of phosphorylated DEPTOR, promoting its stability and leading to decreased mTORC1 signaling (PubMed : 35216969).. (Microbial infection) Contributes to the overall stabilization and trans-activation capability of the herpesvirus 1 trans-acting transcriptional protein ICP0/VMW110 during HSV-1 infection.. (Microbial infection) Upon infection with Epstein-Barr virus, the interaction with viral EBNA1 increases the association of USP7 with PML proteins, which is required for the polyubiquitylation and degradation of PML.

See full target information USP7

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HAUSP / USP7 antibody [EPR4254] - BSA and Azide free

Western blot - Anti-HAUSP / USP7 antibody [EPR4254] - BSA and Azide free (AB247763)

Western blot - Anti-HAUSP / USP7 antibody [EPR4254] - BSA and Azide free (AB247763)

Western blot - Anti-HAUSP / USP7 antibody [EPR4254] - BSA and Azide free (AB247763)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise