AB137613

Anti-HAX1 antibody

Name: Anti-HAX1 antibody (ab137613) | Abcam
Brand: Abcam Limited
SKU: AB137613

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(8 Publications)

Rabbit Polyclonal HAX1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Rat, Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HAX1 aa 1-300.

View Alternative Names

HS1BP1, HAX1, HCLS1-associated protein X-1, HS1-associating protein X-1, HS1-binding protein 1, HAX-1, HSP1BP-1

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-HAX1 antibody (AB137613)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-HAX1 antibody (AB137613)

ab137613 at a 1/500 dilution, staining HAX1 in methanol-fixed A431 cells by Immunofluorescence (green).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HAX1 antibody (AB137613)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HAX1 antibody (AB137613)

ab137613, at a 1/100 dilution, staining HAX1 in paraffin embedded NCIN87 xenograft tissue by Immunohistochemistry.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HAX1 antibody (AB137613)

Immunofluorescence analysis of HeLa cells fixed in ice-cold MeOH for 5 min labelling HAX1 protein at cell membrane using ab137613 at a 1/500 dilution (Green).
alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody at a 1/1000 dilution (Red). DAPI was used for nuclear staining (Blue).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HAX1 antibody (AB137613)

Immunofluorescence analysis of A431 cells fixed in ice-cold MeOH for 5 min labelling HAX1 protein at cytoplasm using ab137613 at a 1/500 dilution (Green).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HAX1 antibody (AB137613)

Paraffin embedded rat ovary tissue stained for HAX1 using ab137613 at 1/500 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-HAX1 antibody (AB137613)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-HAX1 antibody (ab137613) at 1/1000 dilution

Lane 1:

293T whole cell lysates at 30 µg

Lane 2:

A431 whole cell lysates at 30 µg

Lane 3:

HeLa whole cell lysates at 30 µg

Lane 4:

HepG2 whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 32 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human HAX1 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

O00165

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 10% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HAX1 also known as HS-1 associated protein X-1 is a multifunctional protein with a mass of approximately 35 kDa. It plays a significant role in cellular activities by regulating various signaling pathways and maintaining cellular homeostasis. The expression of HAX1 is found in several tissues including the brain heart and skeletal muscle indicating its diverse functional importance across different cell types.

Biological function summary

HAX1 contributes to the stabilization of mitochondria by preventing apoptosis. It interacts with other proteins to form complexes that support cell survival. HAX1's involvement in mitochondrial functions highlights its role in regulating energy production and cellular stress responses. Its capacity to inhibit programmed cell death makes it essential for maintaining cell integrity under stress conditions.

Pathways

HAX1 is integral to the mitochondrial apoptosis pathway and also plays a part in the cytoskeletal signaling pathway. In the apoptosis pathway HAX1 interacts with proteins like BCL-2 to inhibit cell death ensuring cell survival. Additionally in the cytoskeletal signaling pathway HAX1 associates with cytoskeletal proteins aiding in the reorganization of cell structure and enhancing cellular processes like migration.

HAX1 mutations or dysregulation link to severe congenital neutropenia and some neurological disorders. Mutations in HAX1 disrupt the apoptosis inhibition pathway causing a loss in neutrophil count which results in severe congenital neutropenia. This condition is often associated with proteins like ELANE. In some neurological disorders altered HAX1 levels can disturb cellular signaling and survival leading to cellular dysfunction.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Recruits the Arp2/3 complex to the cell cortex and regulates reorganization of the cortical actin cytoskeleton via its interaction with KCNC3 and the Arp2/3 complex (PubMed : 26997484). Slows down the rate of inactivation of KCNC3 channels (PubMed : 26997484). Promotes GNA13-mediated cell migration. Involved in the clathrin-mediated endocytosis pathway. May be involved in internalization of ABC transporters such as ABCB11. May inhibit CASP9 and CASP3. Promotes cell survival. May regulate intracellular calcium pools.

See full target information HAX1

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 28:e18110 PubMed38164042

2024

Cav-1 regulates the bile salt export pump on the canalicular membrane of hepatocytes by PKCα-associated signalling under cholesterol stimulation.

Applications

Unspecified application

Species

Unspecified reactive species

Liwei Pang,Meiying Cui,Shuodong Wu,Jing Kong

Nature communications 14:2028 PubMed37041140

2023

Structural basis of impaired disaggregase function in the oxidation-sensitive SKD3 mutant causing 3-methylglutaconic aciduria.

Applications

Unspecified application

Species

Unspecified reactive species

Sukyeong Lee,Sang Bum Lee,Nuri Sung,Wendy W Xu,Changsoo Chang,Hyun-Eui Kim,Andre Catic,Francis T F Tsai

Advances in clinical and experimental medicine : official organ Wroclaw Medical University 31:689-699 PubMed35302298

2022

Effect of different expression patterns of HAX-1 on the proliferation and apoptosis of human astrocyte.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaopeng Xia,Xiaojian Zhu,Shu Zhang,Xiaoxia Zhang,Yan Wang

International journal of molecular medicine 46:1849-1861 PubMed33000203

2020

MicroRNA‑125a‑5p regulates liver cancer cell growth, migration and invasion and EMT by targeting HAX1.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongming Zha,Jie Li

eLife 9: PubMed32573439

2020

Skd3 (human ClpB) is a potent mitochondrial protein disaggregase that is inactivated by 3-methylglutaconic aciduria-linked mutations.

Applications

Unspecified application

Species

Unspecified reactive species

Ryan R Cupo,James Shorter

BioMed research international 2020:4914707 PubMed32104694

2020

miR-654-5p Targets HAX-1 to Regulate the Malignancy Behaviors of Colorectal Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Fuda Huang,Xianjian Wu,Mingwei Wei,Houji Guo,Hua Li,Zesheng Shao,Yi Wu,Jian Pu

Cancer biology & therapy 20:1223-1233 PubMed31132019

2019

Hematopoietic-substrate-1 associated protein X-1 (HAX-1) regulates liver cancer cells growth, metastasis, and angiogenesis through Akt.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenyu Wu,Xiangnan Ai,Hao Hu,Siqi Wang,Yang Wang,Feng Kang,Caiguo Ouyang,Jiye Zhu

Journal of virology 92: PubMed29563290

2018

H5N1 Influenza A Virus PB1-F2 Relieves HAX-1-Mediated Restriction of Avian Virus Polymerase PA in Human Lung Cells.

Applications

Unspecified application

Species

Unspecified reactive species

B Mazel-Sanchez,I Boal-Carvalho,F Silva,R Dijkman,M Schmolke

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HAX1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-HAX1 antibody (AB137613)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HAX1 antibody (AB137613)

Immunocytochemistry/ Immunofluorescence - Anti-HAX1 antibody (AB137613)

Immunocytochemistry/ Immunofluorescence - Anti-HAX1 antibody (AB137613)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HAX1 antibody (AB137613)

Western blot - Anti-HAX1 antibody (AB137613)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Multiplex Kits

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise