AB221884

Anti-HB9/HLXB9/MNX1 antibody

Name: Anti-HB9/HLXB9/MNX1 antibody (ab221884) | Abcam
Brand: Abcam Limited
SKU: AB221884
Price: 420 USD
Availability: InStock

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(7 Publications)

Anti-HB9/HLXB9/MNX1 antibody (ab221884) is a rabbit polyclonal antibody detecting HB9/HLXB9/MNX1 in ICC/IF. Suitable for Human.

View Alternative Names

HLXB9, MNX1, Motor neuron and pancreas homeobox protein 1, Homeobox protein HB9

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-HB9/HLXB9/MNX1 antibody (AB221884)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HB9/HLXB9/MNX1 antibody (AB221884)

PFA-fixed, Triton X-100 permeabilized MCF7 (human breast adenocarcinoma cell line) cells stained for HB9/HLXB9/MNX1 (green) using ab221884 at 4 μg/ml in ICC/IF.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human MNX1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P50219

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }

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Product details

What is this antibody validated in?
Anti-HB9/HLXB9/MNX1 antibody (ab221884) is a rabbit polyclonal antibody and is validated for use in Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HB9 also known as HLXB9 and MNX1 plays a mechanical role as a transcription factor in spinal motor neurons and pancreatic islets. With a molecular mass of approximately 40 kDa HB9 expression occurs mainly in the developing central nervous system and pancreas. This protein regulates the expression of genes involved in motor neuron identity and pancreatic beta-cell development. Experts often use HB9 as a marker in developmental biology due to its specific expression pattern in the spinal cord and pancreas.

Biological function summary

HB9 regulates the development and maintenance of motor neurons and pancreatic beta-cells being essential for normal function. It does not form part of a larger protein complex but interacts with various DNA sequences to influence gene transcription. Its activity plays a critical role in ensuring the proper differentiation of cell types during embryonic development.

Pathways

HB9 is integral to both the motor neuron differentiation pathway and pancreas morphogenesis. The Sonic Hedgehog (SHH) signaling pathway interacts with HB9 during the specification of spinal motor neurons. In the pancreas HB9 regulates pathways involving insulin-producing beta-cell formation alongside other transcription factors like PDX1 which closely interact with HB9 functions in pancreas development.

HB9 mutations or misregulation connect with conditions such as Currarino syndrome and type 2 diabetes. Currarino syndrome results from mutations affecting HLXB9 function leading to malformations and neuropathologies. In diabetes impaired HB9 function can disturb beta-cell formation or function exacerbating glucose metabolism disorders. HB9's link with PDX1 further highlights its relevance in diabetes as both proteins play roles in pancreatic development and function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor (By similarity). Recognizes and binds to the regulatory elements of target genes, such as visual system homeobox CHX10, negatively modulating transcription (By similarity). Plays a role in establishing motor neuron identity, in concert with LIM domain transcription factor LMO4 (By similarity). Involved in negatively modulating transcription of interneuron genes in motor neurons, acting, at least in part, by blocking regulatory sequence interactions of the ISL1-LHX3 complex (By similarity). Involved in pancreas development and function; may play a role in pancreatic cell fate specification (By similarity).

See full target information MNX1

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Medicine 5:101559 PubMed38744275

2024

The adipose-neural axis is involved in epicardial adipose tissue-related cardiac arrhythmias.

Applications

Unspecified application

Species

Unspecified reactive species

Yubao Fan,Shanshan Huang,Suhua Li,Bingyuan Wu,Qi Zhao,Li Huang,Zhenda Zheng,Xujing Xie,Jia Liu,Weijun Huang,Jiaqi Sun,Xiulong Zhu,Jieming Zhu,Andy Peng Xiang,Weiqiang Li

Haematologica 109:725-739 PubMed37317878

2024

Aberrant MNX1 expression associated with t(7;12)(q36;p13) pediatric acute myeloid leukemia induces the disease through altering histone methylation.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmed Waraky,Anders Östlund,Tina Nilsson,Dieter Weichenhan,Pavlo Lutsik,Marion Bähr,Joschka Hey,Gürcan Tunali,Jenni Adamsson,Susanna Jacobsson,Mohammad Hamdy Abdelrazak Morsy,Susann Li,Linda Fogelstrand,Christoph Plass,Lars Palmqvist

Cell research 34:193-213 PubMed38177242

2024

Decoding the spatiotemporal regulation of transcription factors during human spinal cord development.

Applications

Unspecified application

Species

Unspecified reactive species

Yingchao Shi,Luwei Huang,Hao Dong,Meng Yang,Wenyu Ding,Xiang Zhou,Tian Lu,Zeyuan Liu,Xin Zhou,Mengdi Wang,Bo Zeng,Yinuo Sun,Suijuan Zhong,Bosong Wang,Wei Wang,Chonghai Yin,Xiaoqun Wang,Qian Wu

Nature protocols 18:3918-3973 PubMed37985878

2023

Co-fractionation-mass spectrometry to characterize native mitochondrial protein assemblies in mammalian neurons and brain.

Applications

Unspecified application

Species

Unspecified reactive species

Mara Zilocchi,Matineh Rahmatbakhsh,Mohamed Taha Moutaoufik,Kirsten Broderick,Alla Gagarinova,Matthew Jessulat,Sadhna Phanse,Hiroyuki Aoki,Khaled A Aly,Mohan Babu

Neural regeneration research 19:205-211 PubMed37488868

2023

The p.S87L mutation reduces proliferation of pluripotent stem cells derived from a patient with the spinal muscular atrophy-like phenotype by inhibiting proliferation-related signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Sen Zeng,Honglan Yang,Binghao Wang,Yongzhi Xie,Ke Xu,Lei Liu,Wanqian Cao,Xionghao Liu,Beisha Tang,Mujun Liu,Ruxu Zhang

eLife 12: PubMed36602025

2023

Single-cell profiling of lncRNAs in human germ cells and molecular analysis reveals transcriptional regulation of LNC1845 on LHX8.

Applications

Unspecified application

Species

Unspecified reactive species

Nan Wang,Jing He,Xiaoyu Feng,Shengyou Liao,Yi Zhao,Fuchou Tang,Kehkooi Kee

Frontiers in integrative neuroscience 16:855071 PubMed35669734

2022

Validation of Functional Connectivity of Engineered Neuromuscular Junction With Recombinant Monosynaptic Pseudotyped ΔG-Rabies Virus Tracing.

Applications

Unspecified application

Species

Unspecified reactive species

Ulrich Stefan Bauer,Vegard Fiskum,Rajeevkumar Raveendran Nair,Rosanne van de Wijdeven,Clifford Kentros,Ioanna Sandvig,Axel Sandvig

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-HB9/HLXB9/MNX1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-HB9/HLXB9/MNX1 antibody (AB221884)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise