AB92606

Anti-HB9/HLXB9/MNX1 antibody

Name: Anti-HB9/HLXB9/MNX1 antibody (ab92606) | Abcam
Brand: Abcam Limited
SKU: AB92606
Price: 525 USD
Availability: InStock
Rating: 1 (2 reviews)

(2 Reviews)

(11 Publications)

Rabbit Polyclonal Motor neuron and pancreas homeobox protein 1 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 11 publications.

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HB9/HLXB9/MNX1 antibody (AB92606)

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HB9/HLXB9/MNX1 antibody (AB92606)

Immunohistochemistry analysis of HB9/HLXB9/MNX1 expression in formalin-fixed, paraffin-embedded Mouse pancreas tissue using : an isotype control (1) or ab92606 at 5μg/ml (2 and 3).

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Western blot - Anti-HB9/HLXB9/MNX1 antibody (AB92606)

All lanes:

Western blot - Anti-HB9/HLXB9/MNX1 antibody (ab92606) at 0.5 µg/mL

Lane 1:

MOLT 4 cell lysate

Lane 2:

MOLT 4 cell lysate with immunizing peptide

Secondary

All lanes:

HRP-conjugated Goat anti-Rabbit IgG

Predicted band size: 40 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HB9 also known as HLXB9 and MNX1 plays a mechanical role as a transcription factor in spinal motor neurons and pancreatic islets. With a molecular mass of approximately 40 kDa HB9 expression occurs mainly in the developing central nervous system and pancreas. This protein regulates the expression of genes involved in motor neuron identity and pancreatic beta-cell development. Experts often use HB9 as a marker in developmental biology due to its specific expression pattern in the spinal cord and pancreas.

Biological function summary

HB9 regulates the development and maintenance of motor neurons and pancreatic beta-cells being essential for normal function. It does not form part of a larger protein complex but interacts with various DNA sequences to influence gene transcription. Its activity plays a critical role in ensuring the proper differentiation of cell types during embryonic development.

Pathways

HB9 is integral to both the motor neuron differentiation pathway and pancreas morphogenesis. The Sonic Hedgehog (SHH) signaling pathway interacts with HB9 during the specification of spinal motor neurons. In the pancreas HB9 regulates pathways involving insulin-producing beta-cell formation alongside other transcription factors like PDX1 which closely interact with HB9 functions in pancreas development.

HB9 mutations or misregulation connect with conditions such as Currarino syndrome and type 2 diabetes. Currarino syndrome results from mutations affecting HLXB9 function leading to malformations and neuropathologies. In diabetes impaired HB9 function can disturb beta-cell formation or function exacerbating glucose metabolism disorders. HB9's link with PDX1 further highlights its relevance in diabetes as both proteins play roles in pancreatic development and function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Motor neuron and pancreas homeobox protein 1

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2403077 PubMed39912421

2025

Loss of MNX1 Sensitizes Tumors to Cytotoxic T Cells by Degradation of PD-L1 mRNA.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengzheng Li,Lei Chen,Ge Zhang,Shuang Wang,Enhang Xu,Jinglei Teng,Jiancheng Xu,Fang Peng,Qingjie Min,Zhuoya Wang,Shujuan Shao,Lianmei Zhao,Baoen Shan,Yang Wang,Qimin Zhan,Xuefeng Liu

Acta neuropathologica 148:45 PubMed39305312

2024

TDP-43 regulates LC3ylation in neural tissue through ATG4B cryptic splicing inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Pascual Torres,Santiago Rico-Rios,Miriam Ceron-Codorniu,Marta Santacreu-Vilaseca,David Seoane-Miraz,Yahya Jad,Victòria Ayala,Guillermo Mariño,Maria Beltran,Maria P Miralles,Pol Andrés-Benito,Joaquin Fernandez-Irigoyen,Enrique Santamaria,Carlos López-Otín,Rosa M Soler,Monica Povedano,Isidro Ferrer,Reinald Pamplona,Matthew J A Wood,Miguel A Varela,Manuel Portero-Otin

Journal of Cancer 14:2739-2750 PubMed37779874

2023

A Positive Feedback Loop of E2F4-Mediated Activation of MNX1 Regulates Tumour Progression in Colorectal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jia-Ke Li,Hai Liu,Hui-Wen Zhang,Jun Li,Zhuo-Tao Liang

Cell death discovery 9:113 PubMed37019880

2023

ERK MAPK signaling pathway inhibition as a potential target to prevent autophagy alterations in Spinal Muscular Atrophy motoneurons.

Applications

Unspecified application

Species

Unspecified reactive species

Alba Sansa,Maria P Miralles,Maria Beltran,Ferran Celma-Nos,Jordi Calderó,Ana Garcera,Rosa M Soler

Frontiers in cellular neuroscience 16:1054270 PubMed36619669

2022

Survival motor neuron protein and neurite degeneration are regulated by Gemin3 in spinal muscular atrophy motoneurons.

Applications

Unspecified application

Species

Unspecified reactive species

Maria P Miralles,Alba Sansa,Maria Beltran,Rosa M Soler,Ana Garcera

Acta neuropathologica communications 9:122 PubMed34217376

2021

Spinal Muscular Atrophy autophagy profile is tissue-dependent: differential regulation between muscle and motoneurons.

Applications

Unspecified application

Species

Unspecified reactive species

Alba Sansa,Ivan Hidalgo,Maria P Miralles,Sandra de la Fuente,M Jose Perez-Garcia,Francina Munell,Rosa M Soler,Ana Garcera

Neurobiology of disease 155:105366 PubMed33845129

2021

Intracellular pathways involved in cell survival are deregulated in mouse and human spinal muscular atrophy motoneurons.

Applications

Unspecified application

Species

Unspecified reactive species

Alba Sansa,Sandra de la Fuente,Joan X Comella,Ana Garcera,Rosa M Soler

Frontiers in cell and developmental biology 8:593106 PubMed33490061

2021

Modeling Poliovirus Infection Using Human Engineered Neural Tissue Enriched With Motor Neuron Derived From Embryonic Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Érika Cosset,Youssef Hibaoui,Sten Ilmjärv,Pierre-Yves Dietrich,Caroline Tapparel,Karl-Heinz Krause

Cell death & disease 11:487 PubMed32587237

2020

Calpain system is altered in survival motor neuron-reduced cells from in vitro and in vivo spinal muscular atrophy models.

Applications

Unspecified application

Species

Unspecified reactive species

Sandra de la Fuente,Alba Sansa,Iván Hidalgo,Nuria Vivancos,Ricardo Romero-Guevara,Ana Garcera,Rosa M Soler

Nature protocols 15:421-449 PubMed31932771

2020

On-chip 3D neuromuscular model for drug screening and precision medicine in neuromuscular disease.

Applications

Unspecified application

Species

Unspecified reactive species

Tatsuya Osaki,Sebastien G M Uzel,Roger D Kamm

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-HB9/HLXB9/MNX1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HB9/HLXB9/MNX1 antibody (AB92606)

Western blot - Anti-HB9/HLXB9/MNX1 antibody (AB92606)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

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