AB79541

Anti-HB9/HLXB9/MNX1 antibody [EPR3342]

RabMAb
Recombinant
What is this?

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(7 Publications)

Rabbit Recombinant Monoclonal HB9/HLXB9/MNX1 antibody. Suitable for WB and reacts with Human samples. Cited in 7 publications.

View Alternative Names

HLXB9, MNX1, Motor neuron and pancreas homeobox protein 1, Homeobox protein HB9

1 Images

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Western blot - Anti-HB9/HLXB9/MNX1 antibody [EPR3342] (AB79541)

All lanes:

Western blot - Anti-HB9/HLXB9/MNX1 antibody [EPR3342] (ab79541) at 1/200000 dilution

Lane 1:

Molt-4 cell lysate at 10 µg

Lane 2:

Raji cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 40 kDa

Observed band size: 48 kDa

false

Carrier free

Anti-HB9/HLXB9/MNX1 antibody [EPR3342] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3342

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

There have been conflicting results with this antibody in IHC-P. PMID 21484430 reported specific labeling in IHC-P, but internally we were unable to reproduce these results. Thus, we have removed IHC-P as a guaranteed application, and we welcome any further feedback from customers using this antibody in IHC-P.

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Reactivity data

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HB9 also known as HLXB9 and MNX1 plays a mechanical role as a transcription factor in spinal motor neurons and pancreatic islets. With a molecular mass of approximately 40 kDa HB9 expression occurs mainly in the developing central nervous system and pancreas. This protein regulates the expression of genes involved in motor neuron identity and pancreatic beta-cell development. Experts often use HB9 as a marker in developmental biology due to its specific expression pattern in the spinal cord and pancreas.

Biological function summary

HB9 regulates the development and maintenance of motor neurons and pancreatic beta-cells being essential for normal function. It does not form part of a larger protein complex but interacts with various DNA sequences to influence gene transcription. Its activity plays a critical role in ensuring the proper differentiation of cell types during embryonic development.

Pathways

HB9 is integral to both the motor neuron differentiation pathway and pancreas morphogenesis. The Sonic Hedgehog (SHH) signaling pathway interacts with HB9 during the specification of spinal motor neurons. In the pancreas HB9 regulates pathways involving insulin-producing beta-cell formation alongside other transcription factors like PDX1 which closely interact with HB9 functions in pancreas development.

HB9 mutations or misregulation connect with conditions such as Currarino syndrome and type 2 diabetes. Currarino syndrome results from mutations affecting HLXB9 function leading to malformations and neuropathologies. In diabetes impaired HB9 function can disturb beta-cell formation or function exacerbating glucose metabolism disorders. HB9's link with PDX1 further highlights its relevance in diabetes as both proteins play roles in pancreatic development and function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor (By similarity). Recognizes and binds to the regulatory elements of target genes, such as visual system homeobox CHX10, negatively modulating transcription (By similarity). Plays a role in establishing motor neuron identity, in concert with LIM domain transcription factor LMO4 (By similarity). Involved in negatively modulating transcription of interneuron genes in motor neurons, acting, at least in part, by blocking regulatory sequence interactions of the ISL1-LHX3 complex (By similarity). Involved in pancreas development and function; may play a role in pancreatic cell fate specification (By similarity).

See full target information MNX1

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Stem cells international 2022:1320950 PubMed36530489

2022

The Efficiency of Direct Maturation: the Comparison of Two hiPSC Differentiation Approaches into Motor Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Catherine Schaefers,Simone Rothmiller,Horst Thiermann,Theo Rein,Annette Schmidt

Frontiers in oncology 10:1307 PubMed32850410

2020

MNX1 Promotes Malignant Progression of Cervical Cancer via Repressing the Transcription of p21.

Applications

Unspecified application

Species

Unspecified reactive species

Biqing Zhu,Yaqin Wu,Jing Luo,Quanli Zhang,Jian Huang,Qian Li,Lin Xu,Emei Lu,Binhui Ren

International journal of molecular sciences 20: PubMed31159418

2019

Differentiation of Motor Neuron-Like Cells from Tonsil-Derived Mesenchymal Stem Cells and Their Possible Application to Neuromuscular Junction Formation.

Applications

Unspecified application

Species

Unspecified reactive species

Saeyoung Park,Ji Yeon Kim,Seoha Myung,Namhee Jung,Yeonzi Choi,Sung-Chul Jung

Science translational medicine 4:145ra104 PubMed22855461

2012

Drug screening for ALS using patient-specific induced pluripotent stem cells.

Applications

Unspecified application

Species

Human

Naohiro Egawa,Shiho Kitaoka,Kayoko Tsukita,Motoko Naitoh,Kazutoshi Takahashi,Takuya Yamamoto,Fumihiko Adachi,Takayuki Kondo,Keisuke Okita,Isao Asaka,Takashi Aoi,Akira Watanabe,Yasuhiro Yamada,Asuka Morizane,Jun Takahashi,Takashi Ayaki,Hidefumi Ito,Katsuhiro Yoshikawa,Satoko Yamawaki,Shigehiko Suzuki,Dai Watanabe,Hiroyuki Hioki,Takeshi Kaneko,Kouki Makioka,Koichi Okamoto,Hiroshi Takuma,Akira Tamaoka,Kazuko Hasegawa,Takashi Nonaka,Masato Hasegawa,Akihiro Kawata,Minoru Yoshida,Tatsutoshi Nakahata,Ryosuke Takahashi,Maria C N Marchetto,Fred H Gage,Shinya Yamanaka,Haruhisa Inoue

Molecular and cellular biology 32:126-38 PubMed22037760

2011

A role for SMN exon 7 splicing in the selective vulnerability of motor neurons in spinal muscular atrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Matteo Ruggiu,Vicki L McGovern,Francesco Lotti,Luciano Saieva,Darrick K Li,Shingo Kariya,Umrao R Monani,Arthur H M Burghes,Livio Pellizzoni

Virchows Archiv : an international journal of path 458:697-708 PubMed21484430

2011

The homeobox gene HLXB9 is upregulated in a morphological subset of poorly differentiated hepatocellular carcinoma.

Applications

IHC-P, WB

Species

Human, Human

Ludwig Wilkens,Rolf Jaggi,Caroline Hammer,Daniel Inderbitzin,Olivier Giger,Nils von Neuhoff

BMC neuroscience 12:25 PubMed21385431

2011

Proteomic assessment of a cell model of spinal muscular atrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Chia-Yen Wu,Dosh Whye,Lisa Glazewski,Leila Choe,Douglas Kerr,Kelvin H Lee,Robert W Mason,Wenlan Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HB9/HLXB9/MNX1 antibody [EPR3342]

Western blot - Anti-HB9/HLXB9/MNX1 antibody [EPR3342] (AB79541)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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