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AB247504

Anti-HB9/HLXB9/MNX1 antibody [EPR3342] - BSA and Azide free

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Rabbit Recombinant Monoclonal HB9/HLXB9/MNX1 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

HLXB9, MNX1, Motor neuron and pancreas homeobox protein 1, Homeobox protein HB9

1 Images
Western blot - Anti-HB9/HLXB9/MNX1 antibody [EPR3342] - BSA and Azide free (AB247504)
  • WB

Unknown

Western blot - Anti-HB9/HLXB9/MNX1 antibody [EPR3342] - BSA and Azide free (AB247504)

This data was developed using ab79541, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-HB9/HLXB9/MNX1 antibody [EPR3342] (<a href='/en-us/products/primary-antibodies/hb9-hlxb9-mnx1-antibody-epr3342-ab79541'>ab79541</a>) at 1/200000 dilution

Lane 1:

Molt-4 cell lysate at 10 µg

Lane 2:

Raji cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 40 kDa

Observed band size: 48 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3342

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

There have been conflicting results with this antibody in IHC-P. PMID 21484430 reported specific labeling in IHC-P, but internally we were unable to reproduce these results. Thus, we have removed IHC-P as a guaranteed application, and we welcome any further feedback from customers using this antibody in IHC-P.

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Secondary Antibodies

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Reactivity data

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Product details

ab247504 is the carrier-free version of ab79541.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HB9 also known as HLXB9 and MNX1 plays a mechanical role as a transcription factor in spinal motor neurons and pancreatic islets. With a molecular mass of approximately 40 kDa HB9 expression occurs mainly in the developing central nervous system and pancreas. This protein regulates the expression of genes involved in motor neuron identity and pancreatic beta-cell development. Experts often use HB9 as a marker in developmental biology due to its specific expression pattern in the spinal cord and pancreas.
Biological function summary

HB9 regulates the development and maintenance of motor neurons and pancreatic beta-cells being essential for normal function. It does not form part of a larger protein complex but interacts with various DNA sequences to influence gene transcription. Its activity plays a critical role in ensuring the proper differentiation of cell types during embryonic development.

Pathways

HB9 is integral to both the motor neuron differentiation pathway and pancreas morphogenesis. The Sonic Hedgehog (SHH) signaling pathway interacts with HB9 during the specification of spinal motor neurons. In the pancreas HB9 regulates pathways involving insulin-producing beta-cell formation alongside other transcription factors like PDX1 which closely interact with HB9 functions in pancreas development.

HB9 mutations or misregulation connect with conditions such as Currarino syndrome and type 2 diabetes. Currarino syndrome results from mutations affecting HLXB9 function leading to malformations and neuropathologies. In diabetes impaired HB9 function can disturb beta-cell formation or function exacerbating glucose metabolism disorders. HB9's link with PDX1 further highlights its relevance in diabetes as both proteins play roles in pancreatic development and function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor (By similarity). Recognizes and binds to the regulatory elements of target genes, such as visual system homeobox CHX10, negatively modulating transcription (By similarity). Plays a role in establishing motor neuron identity, in concert with LIM domain transcription factor LMO4 (By similarity). Involved in negatively modulating transcription of interneuron genes in motor neurons, acting, at least in part, by blocking regulatory sequence interactions of the ISL1-LHX3 complex (By similarity). Involved in pancreas development and function; may play a role in pancreatic cell fate specification (By similarity).
See full target information MNX1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com