AB228709

Anti-HBE antibody - N-terminal

Name: Anti-HBE antibody - N-terminal (ab228709) | Abcam
Brand: Abcam Limited
SKU: AB228709
Price: 510 USD
Availability: InStock

Be the first to review this product! Submit a review

(2 Publications)

Rabbit Polyclonal HBE antibody. N-terminal. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human HBE1.

View Alternative Names

HBE, HBE1, Hemoglobin subunit epsilon, Epsilon-globin, Hemoglobin epsilon chain

4 Images

Supplier Data

Western blot - Anti-HBE antibody - N-terminal (AB228709)

Samples were separated by 15% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-HBE antibody - N-terminal (ab228709) at 1/1000 dilution

Lane 1:

K562 whole cell extracts at 30 µg

Lane 2:

239T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 16 kDa

false

Supplier Data

Western blot - Anti-HBE antibody - N-terminal (AB228709)

15% SDS-PAGE gel.

CFA : Complete Freunds adjuvant

All lanes:

Western blot - Anti-HBE antibody - N-terminal (ab228709) at 1/500 dilution

Lane 1:

Untreated Mouse leukocyte tissue extract at 30 µg

Lane 2:

CFA-treated mouse leukocyte tissue extract at 30 µg

Predicted band size: 16 kDa

true

Supplier Data

Western blot - Anti-HBE antibody - N-terminal (AB228709)

Samples were separated by 15% SDS PAGE.

All lanes:

Western blot - Anti-HBE antibody - N-terminal (ab228709) at 1/1000 dilution

All lanes:

Mouse spleeen tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 16 kDa

false

Supplier Data

Western blot - Anti-HBE antibody - N-terminal (AB228709)

Samples were separated by 15% SDS PAGE.

All lanes:

Western blot - Anti-HBE antibody - N-terminal (ab228709) at 1/1000 dilution

All lanes:

Rat spleen tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 16 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

Synthetic Peptide within Human HBE1. The exact immunogen used to generate this antibody is proprietary information.

P02100

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The HBE protein also known as hepatitis B virus e antigen (HBeAg) displays significant mechanical functions in the viral life cycle. It has a mass of approximately 17–25 kDa and expresses mainly in the cells of the liver during hepatitis B virus replication. HBE gets produced during the conversion of the precore protein into its mature form which allows it to function effectively as a viral antigen. 'Anti-HBE' and 'anti-HBeAg' antibodies target this antigen. The protein plays a role in immune tolerance mechanisms by modulating host immune responses against the hepatitis B virus.

Biological function summary

The HBE protein contributes to the persistence of hepatitis B virus infection. It's part of the viral replication complex and helps maintain viral chronicity by repressing immune activation. The presence of HBE in the host leads to a reduction in the recognition of infected hepatocytes by the immune system allowing the virus to persist longer. Identifying the presence of 'HBE antigen' and 'anti-HBE' antibodies helps monitor the progression of hepatitis B infection. This antigen is a marker for active viral replication and infectiousness.

Pathways

HBE integrates into the hepatitis B virus life cycle and immune response pathways. It involves the regulation of host immune responses particularly the adaptive immune system's recognition and clearance of infected hepatocytes. HBeAg influences the pathways by interacting with proteins related to immune checkpoints ensuring viral persistence. These interactions alter the balance of pro-inflammatory and anti-inflammatory signals affecting the body's ability to mount an effective immune response against the infected liver cells.

HBE plays an important role in chronic hepatitis B infection and liver disease progression. It associates with reduced inflammation in chronic hepatitis B patients often correlating with lower liver damage. However persistent expression of HBE can contribute to the development of hepatitis B-related liver cirrhosis due to prolonged immune suppression. The production of 'anti-HBE' and 'anti-HBeAg' antibodies against HBE indicates the host's immune status and helps in determining the phase of infection. Understanding the interactions between HBE and other hepatic proteins assists clinicians in making decisions regarding hepatitis B treatment strategies.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

The epsilon chain is a beta-type chain of early mammalian embryonic hemoglobin.

See full target information HBE1

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of immunology (Baltimore, Md. : 1950) 208:672-684 PubMed35022275

2022

Hepatitis B and Hepatitis C Virus Infection Promote Liver Fibrogenesis through a TGF-β1-Induced OCT4/Nanog Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wenting Li,Xiaoqiong Duan,Chuanlong Zhu,Xiao Liu,Andre J Jeyarajan,Min Xu,Zeng Tu,Qiuju Sheng,Dong Chen,Chuanwu Zhu,Tuo Shao,Zhimeng Cheng,Shadi Salloum,Esperance A Schaefer,Annie J Kruger,Jacinta A Holmes,Raymond T Chung,Wenyu Lin

Molecular cell 81:3128-3144.e7 PubMed34216544

2021

Replication gaps are a key determinant of PARP inhibitor synthetic lethality with BRCA deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Cong,Min Peng,Arne Nedergaard Kousholt,Wei Ting C Lee,Silviana Lee,Sumeet Nayak,John Krais,Pamela S VanderVere-Carozza,Katherine S Pawelczak,Jennifer Calvo,Nicholas J Panzarino,John J Turchi,Neil Johnson,Jos Jonkers,Eli Rothenberg,Sharon B Cantor

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HBE antibody - N-terminal

Western blot - Anti-HBE antibody - N-terminal (AB228709)

Western blot - Anti-HBE antibody - N-terminal (AB228709)

Western blot - Anti-HBE antibody - N-terminal (AB228709)

Western blot - Anti-HBE antibody - N-terminal (AB228709)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise