AB66792

Anti-HBEGF/DTR antibody [4G10]

Name: Anti-HBEGF/DTR antibody [4G10] (ab66792) | Abcam
Brand: Abcam Limited
SKU: AB66792
Price: 430 USD
Availability: InStock

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(6 Publications)

Mouse Monoclonal HBEGF/DTR antibody. Suitable for IP, WB, ICC/IF and reacts with Transfected cell line - Human, Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HBEGF.

View Alternative Names

DTR, DTS, HEGFL, HBEGF, Proheparin-binding EGF-like growth factor

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-HBEGF/DTR antibody [4G10] (AB66792)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-HBEGF/DTR antibody [4G10] (AB66792)

These images show Vero cells carrying human HG-EGF expression vector (left hand image) and Vero cells carrying mouse HBEGF/DTR expression vector (right hand image). Cells treated with ab 66792, fixed with 4% PFA and reacted with Cys3 conjugated 2nd antibody.

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Immunoprecipitation - Anti-HBEGF/DTR antibody [4G10] (AB66792)

Lane 1 : Vero cell extract, 1μg
Lane 2 : Vero cells carrying Human HBEGF/DTR expression vector, 1μg
Lane 3 : Vero Cells carrying mouse HBEGF/DTR expression vector, 1μg

ab66792 immunoprecipitating HBEGF/DTR in Vero Cells. For western blotting, a HRP conjugated streptavidin was used as the secondary antibody; cell surface was biotinylated.

Lane 1:

Immunoprecipitation - Anti-HBEGF/DTR antibody [4G10] (ab66792)

Lane 1:

Immunoprecipitation - Biotin Anti-HBEGF/DTR antibody [4G10] (<a href='/en-us/products/primary-antibodies/biotin-hbegf-dtr-antibody-4g10-ab66793'>ab66793</a>)

Predicted band size: 23 kDa

Observed band size: 20-38 kDa,41 kDa

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Western blot - Anti-HBEGF/DTR antibody [4G10] (AB66792)

Lanes 1 - 3:

Western blot - Biotin Anti-HBEGF/DTR antibody [4G10] (<a href='/en-us/products/primary-antibodies/biotin-hbegf-dtr-antibody-4g10-ab66793'>ab66793</a>) at 1 µg/mL

Lanes 1 - 3:

Western blot - Anti-HBEGF/DTR antibody [4G10] (ab66792) at 1 µg/mL

Lane 1:

Vero (African green monkey kidney epithelial cell) whole cell lysate

Lane 2:

Vero cell extract from cells carrying human HBEGF/DTR expression vector

Lane 3:

Vero cell extract from cells carrying mouse HBEGF/DTR expression vector

Predicted band size: 23 kDa

Observed band size: 20-28 kDa,41 kDa

false

Biotin

Biotin Anti-HBEGF/DTR antibody [4G10]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

4G10

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

ICC/IF, IP, WB

applications

Immunogen

Recombinant Fragment Protein within Human HBEGF. The exact immunogen used to generate this antibody is proprietary information.

Q99075

Epitope

EGF domain

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5-10 µg/mL", "ICCIF-species-notes": "" }, "Transfected cell line - Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "Use at 2μg/ml", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Produced in serum-free medium and purified by combination of chromatography

Storage buffer

pH: 6 - 8.5 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HBEGF also known as Heparin-binding EGF-like growth factor or Diphtheria Toxin Receptor (DTR) is a growth factor that binds to EGF receptors with its mass approximately 20 kilodaltons. It is a member of the EGF family. Cells secrete HBEGF and it is expressed in many tissues including the heart kidney and lungs. Its expression often increases during proliferation and differentiation making it an important player in tissue growth and repair.

Biological function summary

HBEGF functions as a mitogen and chemotactic agent which means it stimulates cell division and attracts cells to migrate. As part of the EGF family HBEGF activates the EGFR (epidermal growth factor receptor) signaling cascade. This signaling is essential for processes such as wound healing and development. HBEGF also forms complexes with other ligands and receptors enhancing its biological activity in tissues.

Pathways

HBEGF plays a role in the MAPK/ERK signaling pathway and the PI3K/AKT pathway. Both pathways are significant for regulating cell growth survival and differentiation. HBEGF interacts with other proteins like Ras and PI3K in these pathways. By activating these pathways HBEGF influences cellular responses critical for normal physiological processes and recovery mechanisms in cells.

HBEGF has connections with cancer and cardiovascular diseases. Aberrant HBEGF signaling can promote oncogenic processes in cancer relating it to proteins such as HER2 which also engage in similar pathways. In cardiovascular diseases HBEGF overexpression can contribute to smooth muscle cell proliferation an important event in atherosclerosis linking it to other growth factors involved in vascular biology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. Required for normal cardiac valve formation and normal heart function. Promotes smooth muscle cell proliferation. May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.

See full target information HBEGF

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell international 25:219 PubMed40542386

2025

Hsa_circ_0059511 promote glioma cell proliferation and migration through hsa-miR-194-5p/HBEGF axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yajun Jing,Huang-Cheng Shang-Guan,Jiawei Cai,Jinye Su,Wei Huang,Qiu He,Xiaoyong Chen,Dezhi Kang,Zanyi Wu

Science advances 6: PubMed32917630

2020

Attenuated diphtheria toxin mediates siRNA delivery.

Applications

Unspecified application

Species

Unspecified reactive species

Amy E Arnold,Laura J Smith,Greg L Beilhartz,Laura C Bahlmann,Emma Jameson,Roman A Melnyk,Molly S Shoichet

Experimental and therapeutic medicine 13:3021-3031 PubMed28587375

2017

C-X-C motif chemokine ligand 8 promotes endothelial cell homing via the Akt-signal transducer and activator of transcription pathway to accelerate healing of ischemic and hypoxic skin ulcers.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Shen,Peng Zhang,Shanqiang Zhang,Liping Xie,Lijie Yao,Weiya Lang,Jie Lian,Wei Qin,Meng Zhang,Liang Ji

Transfusion and apheresis science : official journ 55:44-52 PubMed27470710

2016

Does smoking, age or gender affect the protein phenotype of extracellular vesicles in plasma?

Applications

Unspecified application

Species

Unspecified reactive species

R Bæk,K Varming,M M Jørgensen

Arteriosclerosis, thrombosis, and vascular biology 32:2484-92 PubMed22859491

2012

Modulation of the cochaperone AHA1 regulates heat-shock protein 90 and endothelial NO synthase activation by vascular endothelial growth factor.

Applications

Unspecified application

Species

Unspecified reactive species

Fanny Desjardins,Chantal Delisle,Jean-Philippe Gratton

Biochemical and biophysical research communication 422:676-80 PubMed22609777

2012

The anti-tumor effect of cross-reacting material 197, an inhibitor of heparin-binding EGF-like growth factor, in human resistant ovarian cancer.

Applications

Unspecified application

Species

Human

Xiao-han Tang,Suo Deng,Meng Li,Mei-song Lu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HBEGF/DTR antibody [4G10]

Immunocytochemistry/ Immunofluorescence - Anti-HBEGF/DTR antibody [4G10] (AB66792)

Immunoprecipitation - Anti-HBEGF/DTR antibody [4G10] (AB66792)

Western blot - Anti-HBEGF/DTR antibody [4G10] (AB66792)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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