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AB104519

Anti-HBS1L antibody

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(1 Publication)

Rabbit Polyclonal HBS1L antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human HBS1L.

View Alternative Names

HBS1, KIAA1038, HBS1L, HBS1-like protein, ERFS

1 Images
Western blot - Anti-HBS1L antibody (AB104519)
  • WB

Supplier Data

Western blot - Anti-HBS1L antibody (AB104519)

Exposure : Unpurify 35 s. Purify 80 s.

Lane 2:

Unpurify Polysera at 1/1000 dilution

Lanes 3 - 4:

Purified Polysera at 1 µg/mL

Lane 1:

Human liver lysate at 25 µg/mL

Lanes 2 and 4:

pCD lysate at 15 µL

Lane 3:

293T lysate at 20 µL

Secondary

All lanes:

Goat anti-rabbit IgG HRP at 1/5000 dilution

Predicted band size: 75 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human HBS1L.

Q9Y450

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HBS1L known also as HBS1-like protein functions as a GTPase associated with translation regulation. It contains motifs essential for GTP binding and hydrolysis. The molecular mass of HBS1L is approximately 73 kDa. Expression of HBS1L can be observed in various tissues with more significance in hematopoietic tissues. It acts as part of cellular machinery involved in maintaining ribosomal integrity and function during translation.
Biological function summary

HBS1L plays a critical role in retaining the fidelity of mRNA translation by participating in ribosome rescue mechanisms. It forms the SURF complex in association with other proteins like eRF1 and Pelota to release stalled ribosomes and continue protein synthesis. This action helps prevent errors during translation ensuring proper polypeptide formation.

Pathways

HBS1L participates significantly in the ribosome recovery and quality control pathways. It becomes an essential player in the non-stop decay pathway wherein it cooperates with Pelota and ABCE1 proteins. This collaboration reflects its importance in the maintenance of cellular homeostasis by managing defective mRNA decay and ribosomal recycling ensuring effective translation termination when regular processes are interrupted.

HBS1L connects notably with beta-thalassemia and sickle cell disease. Its genetic variations often show association with altered fetal hemoglobin levels influencing these conditions. Additionally HBS1L interplays with the BCL11A protein which acts as a repressor of fetal hemoglobin production. This interaction provides insights into potential therapeutic targets for managing hemoglobinopathies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

GTPase component of the Pelota-HBS1L complex, a complex that recognizes stalled ribosomes and triggers the No-Go Decay (NGD) pathway (PubMed : 21448132, PubMed : 23667253, PubMed : 27863242). The Pelota-HBS1L complex recognizes ribosomes stalled at the 3' end of an mRNA and engages stalled ribosomes by destabilizing mRNA in the mRNA channel (PubMed : 27863242). Following mRNA extraction from stalled ribosomes by the SKI complex, the Pelota-HBS1L complex promotes recruitment of ABCE1, which drives the disassembly of stalled ribosomes, followed by degradation of damaged mRNAs as part of the NGD pathway (PubMed : 21448132, PubMed : 32006463).
See full target information HBS1L
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 12:4808 PubMed34376683

2021

A microRNA program regulates the balance between cardiomyocyte hyperplasia and hypertrophy and stimulates cardiac regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Raso,Ellen Dirkx,Vasco Sampaio-Pinto,Hamid El Azzouzi,Ryan J Cubero,Daniel W Sorensen,Lara Ottaviani,Servé Olieslagers,Manon M Huibers,Roel de Weger,Sailay Siddiqi,Silvia Moimas,Consuelo Torrini,Lorena Zentillin,Luca Braga,Diana S Nascimento,Paula A da Costa Martins,Jop H van Berlo,Serena Zacchigna,Mauro Giacca,Leon J De Windt
View all publications
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