AB217067

Anti-HBS1L antibody

Name: Anti-HBS1L antibody (ab217067) | Abcam
Brand: Abcam Limited
SKU: AB217067
Price: 525 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal HBS1L antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human HBS1L aa 150-200.

View Alternative Names

HBS1, KIAA1038, HBS1L, HBS1-like protein, ERFS

2 Images

Supplier Data

Immunoprecipitation - Anti-HBS1L antibody (AB217067)

Detection of HBS1L in immunoprecipitates of Jurkat whole cell lysate prepared using NETN lysis buffer (0.5 or 1 mg for IP, 20% of IP loaded) using ab217067 at 6 μg/mg lysate for IP (Lane 1), and at 0.4 μg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-HBS1L antibody (ab217067)

Predicted band size: 75 kDa

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Supplier Data

Western blot - Anti-HBS1L antibody (AB217067)

All lanes:

Western blot - Anti-HBS1L antibody (ab217067) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysates at 50 µg

Lane 2:

293T whole cell lysates at 50 µg

Lane 3:

Jurkat whole cell lysates at 50 µg

Predicted band size: 75 kDa

true

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human HBS1L aa 150-200. The exact immunogen used to generate this antibody is proprietary information.

Q9Y450

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab217067 was affinity purified using an epitope specific to HBS1L immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HBS1L known also as HBS1-like protein functions as a GTPase associated with translation regulation. It contains motifs essential for GTP binding and hydrolysis. The molecular mass of HBS1L is approximately 73 kDa. Expression of HBS1L can be observed in various tissues with more significance in hematopoietic tissues. It acts as part of cellular machinery involved in maintaining ribosomal integrity and function during translation.

Biological function summary

HBS1L plays a critical role in retaining the fidelity of mRNA translation by participating in ribosome rescue mechanisms. It forms the SURF complex in association with other proteins like eRF1 and Pelota to release stalled ribosomes and continue protein synthesis. This action helps prevent errors during translation ensuring proper polypeptide formation.

Pathways

HBS1L participates significantly in the ribosome recovery and quality control pathways. It becomes an essential player in the non-stop decay pathway wherein it cooperates with Pelota and ABCE1 proteins. This collaboration reflects its importance in the maintenance of cellular homeostasis by managing defective mRNA decay and ribosomal recycling ensuring effective translation termination when regular processes are interrupted.

HBS1L connects notably with beta-thalassemia and sickle cell disease. Its genetic variations often show association with altered fetal hemoglobin levels influencing these conditions. Additionally HBS1L interplays with the BCL11A protein which acts as a repressor of fetal hemoglobin production. This interaction provides insights into potential therapeutic targets for managing hemoglobinopathies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

GTPase component of the Pelota-HBS1L complex, a complex that recognizes stalled ribosomes and triggers the No-Go Decay (NGD) pathway (PubMed : 21448132, PubMed : 23667253, PubMed : 27863242). The Pelota-HBS1L complex recognizes ribosomes stalled at the 3' end of an mRNA and engages stalled ribosomes by destabilizing mRNA in the mRNA channel (PubMed : 27863242). Following mRNA extraction from stalled ribosomes by the SKI complex, the Pelota-HBS1L complex promotes recruitment of ABCE1, which drives the disassembly of stalled ribosomes, followed by degradation of damaged mRNAs as part of the NGD pathway (PubMed : 21448132, PubMed : 32006463).

See full target information HBS1L

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:4491 PubMed40368919

2025

Inherited deficiency of DIAPH1 identifies a DNA double strand break repair pathway regulated by γ-actin.

Applications

Unspecified application

Species

Unspecified reactive species

Beth L Woodward,Sudipta Lahiri,Anoop S Chauhan,Marcos Rios Garcia,Lucy E Goodley,Thomas L Clarke,Mohinder Pal,Angelo Agathanggelou,Satpal S Jhujh,Anil N Ganesh,Fay M Hollins,Valentina Galassi Deforie,Reza Maroofian,Stephanie Efthymiou,Andrea Meinhardt,Christopher G Mathew,Michael A Simpson,Heather C Mefford,Eissa A Faqeih,Sergio D Rosenzweig,Stefano Volpi,Gigliola Di Matteo,Caterina Cancrini,Annarita Scardamaglia,Fiona Shackley,E Graham Davies,Shahnaz Ibrahim,Peter D Arkwright,Maha S Zaki,Tatjana Stankovic,A Malcolm R Taylor,Antonina J Mazur,Nataliya Di Donato,Henry Houlden,Eli Rothenberg,Grant S Stewart

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HBS1L antibody

Immunoprecipitation - Anti-HBS1L antibody (AB217067)

Western blot - Anti-HBS1L antibody (AB217067)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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