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AB224476

Anti-HBS1L antibody

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(1 Publication)

Rabbit Polyclonal HBS1L antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human HBS1L aa 1-100.

View Alternative Names

HBS1, KIAA1038, HBS1L, HBS1-like protein, ERFS

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-HBS1L antibody (AB224476)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HBS1L antibody (AB224476)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for HBS1L (green) using ab224476 at 4 μg/ml in ICC/IF.

Western blot - Anti-HBS1L antibody (AB224476)
  • WB

Supplier Data

Western blot - Anti-HBS1L antibody (AB224476)

All lanes:

Western blot - Anti-HBS1L antibody (ab224476) at 1/100 dilution

Lane 1:

RT4 (human urinary bladder cancer cell line) cell lysate

Lane 2:

U-251 MG sp (human brain glioma cell line) cell lysate

Predicted band size: 75 kDa

true

Western blot - Anti-HBS1L antibody (AB224476)
  • WB

Supplier Data

Western blot - Anti-HBS1L antibody (AB224476)

All lanes:

Western blot - Anti-HBS1L antibody (ab224476) at 1/100 dilution

Lane 1:

NIH/3T3 (mouse embryonic fibroblast cell line) cell lysate

Lane 2:

NBT-II cell lysate

Predicted band size: 75 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human HBS1L aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q9Y450

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HBS1L known also as HBS1-like protein functions as a GTPase associated with translation regulation. It contains motifs essential for GTP binding and hydrolysis. The molecular mass of HBS1L is approximately 73 kDa. Expression of HBS1L can be observed in various tissues with more significance in hematopoietic tissues. It acts as part of cellular machinery involved in maintaining ribosomal integrity and function during translation.
Biological function summary

HBS1L plays a critical role in retaining the fidelity of mRNA translation by participating in ribosome rescue mechanisms. It forms the SURF complex in association with other proteins like eRF1 and Pelota to release stalled ribosomes and continue protein synthesis. This action helps prevent errors during translation ensuring proper polypeptide formation.

Pathways

HBS1L participates significantly in the ribosome recovery and quality control pathways. It becomes an essential player in the non-stop decay pathway wherein it cooperates with Pelota and ABCE1 proteins. This collaboration reflects its importance in the maintenance of cellular homeostasis by managing defective mRNA decay and ribosomal recycling ensuring effective translation termination when regular processes are interrupted.

HBS1L connects notably with beta-thalassemia and sickle cell disease. Its genetic variations often show association with altered fetal hemoglobin levels influencing these conditions. Additionally HBS1L interplays with the BCL11A protein which acts as a repressor of fetal hemoglobin production. This interaction provides insights into potential therapeutic targets for managing hemoglobinopathies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

GTPase component of the Pelota-HBS1L complex, a complex that recognizes stalled ribosomes and triggers the No-Go Decay (NGD) pathway (PubMed : 21448132, PubMed : 23667253, PubMed : 27863242). The Pelota-HBS1L complex recognizes ribosomes stalled at the 3' end of an mRNA and engages stalled ribosomes by destabilizing mRNA in the mRNA channel (PubMed : 27863242). Following mRNA extraction from stalled ribosomes by the SKI complex, the Pelota-HBS1L complex promotes recruitment of ABCE1, which drives the disassembly of stalled ribosomes, followed by degradation of damaged mRNAs as part of the NGD pathway (PubMed : 21448132, PubMed : 32006463).
See full target information HBS1L
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of Cancer 12:467-473 PubMed33391443

2021

MUC 15 Promotes Osteosarcoma Cell Proliferation, Migration and Invasion through Livin, MMP-2/MMP-9 and Wnt/β-Catenin Signal Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Tonglei Chen,Zhenshi Chen,Xiaoning Lian,Weidong Wu,Lei Chu,Shaoru Zhang,Lihui Wang
View all publications
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Product promise

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