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AB157480

Anti-HBXIP antibody [EPR11531(B)]

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(6 Publications )

Rabbit Recombinant Monoclonal HBXIP antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 6 publications.
4 Images
Flow Cytometry (Intracellular) - Anti-HBXIP antibody [EPR11531(B)] (AB157480)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-HBXIP antibody [EPR11531(B)] (AB157480)

Intracellular flow cytometric analysis of permeabilized BxPC-3 cells labeling HBXIP with ab157480 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).

Western blot - Anti-HBXIP antibody [EPR11531(B)] (AB157480)
  • WB

Lab

Western blot - Anti-HBXIP antibody [EPR11531(B)] (AB157480)

Western blot : Anti-HBXIP antibody [EPR11531(B)] ab157480 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 9 kDa in Wild-type A549 cell lysates with no signal observed at this size in LAMTOR5 knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-HBXIP antibody [EPR11531(B)] (ab157480) at 1/1000 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

LAMTOR5 knockout A549 at 20 µg

Lane 3:

PC-3 at 20 µg

Lane 4:

MCF7 at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 10 kDa

Observed band size: 9 kDa,37 kDa

false

Western blot - Anti-HBXIP antibody [EPR11531(B)] (AB157480)
  • WB

Unknown

Western blot - Anti-HBXIP antibody [EPR11531(B)] (AB157480)

All lanes:

Western blot - Anti-HBXIP antibody [EPR11531(B)] (ab157480) at 1/1000 dilution

Lane 1:

BxPC-3 cell lysate at 10 µg

Lane 2:

MCF7 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 10 kDa

false

Western blot - Anti-HBXIP antibody [EPR11531(B)] (AB157480)
  • WB

Unknown

Western blot - Anti-HBXIP antibody [EPR11531(B)] (AB157480)

All lanes:

Western blot - Anti-HBXIP antibody [EPR11531(B)] (ab157480) at 1/1000 dilution

All lanes:

immunoprecipitation pellet from BxPC-3 cell lysate

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 10 kDa

false

  • Carrier free

    Anti-HBXIP antibody [EPR11531(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11531(B)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/500", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HBXIP also known as Hepatitis B X-interacting protein or LAMTOR5 is a protein with a mass of approximately 18 kDa. HBXIP binds to the HIV-1 protease and is involved in the activation of diverse cellular processes. It predominantly expresses in heart kidney liver and skeletal muscle tissues. The protein's mechanical role involves interacting with the hepatitis B virus X protein (HBx) enhancing the replication of hepatitis B virus particles.
Biological function summary

HBXIP impacts multiple cellular functions in a complex manner. It participates in the regulation of centrosome dynamics and cell division. HBXIP interacts with proteins such as survivin in a complex that plays a role in mitosis. HBXIP also influences gene expression by modulating estrogen receptor signaling. These functions make HBXIP a significant component in cellular growth control.

Pathways

HBXIP engages in cell cycle regulation and apoptosis. It affects the mTOR signaling pathway important for cell growth and metabolism. Within this context HBXIP acts alongside proteins like mTOR and RHEB influencing nutrient and energy status in the cell. Furthermore its interaction with the p53 pathway highlights its role in controlling cell survival and the response to DNA damage.

HBXIP displays connections to cancer and viral infections. In cancer HBXIP overexpression correlates with tumor progression and poor patient prognosis commonly seen in breast cancer. Through its influence on nuclear factor-kB (NF-kB) HBXIP associates with inflammatory responses tied to cancer progression. Additionally its relationship with hepatitis B viral oncogenesis highlights a link between HBXIP and liver disorders signaling interaction with the viral HBx protein as a point of mechanism.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

As part of the Ragulator complex it is involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids (PubMed : 22980980, PubMed : 29158492, PubMed : 30181260). Activated by amino acids through a mechanism involving the lysosomal V-ATPase, the Ragulator plays a dual role for the small GTPases Rag (RagA/RRAGA, RagB/RRAGB, RagC/RRAGC and/or RagD/RRAGD) : it (1) acts as a guanine nucleotide exchange factor (GEF), activating the small GTPases Rag and (2) mediates recruitment of Rag GTPases to the lysosome membrane (PubMed : 22980980, PubMed : 28935770, PubMed : 29107538, PubMed : 29158492, PubMed : 30181260). Activated Ragulator and Rag GTPases function as a scaffold recruiting mTORC1 to lysosomes where it is in turn activated (PubMed : 22980980, PubMed : 29158492, PubMed : 30181260). When complexed to BIRC5, interferes with apoptosome assembly, preventing recruitment of pro-caspase-9 to oligomerized APAF1, thereby selectively suppressing apoptosis initiated via the mitochondrial/cytochrome c pathway (PubMed : 12773388).
See full target information LAMTOR5

Alternative Names

HBXIP, XIP, LAMTOR5, Ragulator complex protein LAMTOR5, Hepatitis B virus X-interacting protein, Late endosomal/lysosomal adaptor and MAPK and MTOR activator 5, HBV X-interacting protein, HBX-interacting protein
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 25:186 PubMed37065787

2023

HBXIP knockdown inhibits FHL2 to promote cycle arrest and suppress cervical cancer cell proliferation, invasion and migration.

Applications

Unspecified application

Species

Unspecified reactive species

Xia Gao,Lina Yang

Journal of cellular physiology 236:3863-3880 PubMed33305825

2020

HBXIP drives metabolic reprogramming in hepatocellular carcinoma cells via METTL3-mediated m6A modification of HIF-1α.

Applications

Unspecified application

Species

Unspecified reactive species

Nanmu Yang,Tao Wang,Qingjun Li,Feng Han,Zhengzheng Wang,Ruili Zhu,Jinxue Zhou

Aging 12:24967-24982 PubMed33048840

2020

HBXIP promotes gastric cancer METTL3-mediated MYC mRNA m6A modification.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi Yang,Xiaodi Jiang,Deming Li,Xiaofeng Jiang

Cellular & molecular immunology 17:1063-1076 PubMed31467416

2019

The metabolic regulator Lamtor5 suppresses inflammatory signaling via regulating mTOR-mediated TLR4 degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zhang,Ningtong Zhuang,Xiaoyi Liu,Long He,Yan He,Paween Mahinthichaichan,Hang Zhang,Yanhua Kang,Yin Lu,Qinan Wu,Dakang Xu,Liyun Shi

Experimental and therapeutic medicine 17:4223-4229 PubMed31007753

2019

Hepatitis B virus X protein decreases nephrin expression and induces podocyte apoptosis via activating STAT3.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Yan Lei,Xing-Xing Chen,Yong-Hong Sun,Ming-Dong Gao,Xiao-Xia Hu,Yan-Hong Suo

Carcinogenesis 35:1144-53 PubMed24464787

2014

The oncoprotein HBXIP enhances angiogenesis and growth of breast cancer through modulating FGF8 and VEGF.

Applications

WB

Species

Human

Fabao Liu,Xiaona You,Yue Wang,Qian Liu,Yunxia Liu,Shuqin Zhang,Lingyi Chen,Xiaodong Zhang,Lihong Ye
View all publications
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Product promise

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