Rabbit Recombinant Monoclonal HBXIP antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.
Images
![Western blot - Anti-HBXIP antibody [EPR11531(B)] - BSA and Azide free (AB249343), expandable thumbnail](https://content.abcam.com/products/images/hbxip-antibody-epr11531b-bsa-and-azide-free-ab249343--western-blot-img56978.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-HBXIP antibody [EPR11531(B)] - BSA and Azide free (AB249343)")
![Flow Cytometry (Intracellular) - Anti-HBXIP antibody [EPR11531(B)] - BSA and Azide free (AB249343), expandable thumbnail](https://content.abcam.com/products/images/hbxip-antibody-epr11531b-bsa-and-azide-free-ab249343--flow-cytometry-intracellular-img79264.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-HBXIP antibody [EPR11531(B)] - BSA and Azide free (AB249343)")
![Western blot - Anti-HBXIP antibody [EPR11531(B)] - BSA and Azide free (AB249343), expandable thumbnail](https://content.abcam.com/products/images/hbxip-antibody-epr11531b-bsa-and-azide-free-ab249343--western-blot-img94493.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-HBXIP antibody [EPR11531(B)] - BSA and Azide free (AB249343)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | Flow Cyt (Intra) | |
|---|---|---|---|
| Human | Tested | Not recommended | Tested |
| Mouse | Predicted | Not recommended | Predicted |
| Rat | Predicted | Not recommended | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
As part of the Ragulator complex it is involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids (PubMed:22980980, PubMed:29158492, PubMed:30181260). Activated by amino acids through a mechanism involving the lysosomal V-ATPase, the Ragulator plays a dual role for the small GTPases Rag (RagA/RRAGA, RagB/RRAGB, RagC/RRAGC and/or RagD/RRAGD): it (1) acts as a guanine nucleotide exchange factor (GEF), activating the small GTPases Rag and (2) mediates recruitment of Rag GTPases to the lysosome membrane (PubMed:22980980, PubMed:28935770, PubMed:29107538, PubMed:29158492, PubMed:30181260). Activated Ragulator and Rag GTPases function as a scaffold recruiting mTORC1 to lysosomes where it is in turn activated (PubMed:22980980, PubMed:29158492, PubMed:30181260). When complexed to BIRC5, interferes with apoptosome assembly, preventing recruitment of pro-caspase-9 to oligomerized APAF1, thereby selectively suppressing apoptosis initiated via the mitochondrial/cytochrome c pathway (PubMed:12773388).
Alternative names
HBXIP, XIP, LAMTOR5, Ragulator complex protein LAMTOR5, Hepatitis B virus X-interacting protein, Late endosomal/lysosomal adaptor and MAPK and MTOR activator 5, HBV X-interacting protein, HBX-interacting protein
Recommended products
Rabbit Recombinant Monoclonal HBXIP antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR11531(B)
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab249343 is the carrier-free version of Anti-HBXIP antibody [EPR11531(B)] ab157480.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
HBXIP also known as Hepatitis B X-interacting protein or LAMTOR5 is a protein with a mass of approximately 18 kDa. HBXIP binds to the HIV-1 protease and is involved in the activation of diverse cellular processes. It predominantly expresses in heart kidney liver and skeletal muscle tissues. The protein's mechanical role involves interacting with the hepatitis B virus X protein (HBx) enhancing the replication of hepatitis B virus particles.
- Biological function summary
HBXIP impacts multiple cellular functions in a complex manner. It participates in the regulation of centrosome dynamics and cell division. HBXIP interacts with proteins such as survivin in a complex that plays a role in mitosis. HBXIP also influences gene expression by modulating estrogen receptor signaling. These functions make HBXIP a significant component in cellular growth control.
- Pathways
HBXIP engages in cell cycle regulation and apoptosis. It affects the mTOR signaling pathway important for cell growth and metabolism. Within this context HBXIP acts alongside proteins like mTOR and RHEB influencing nutrient and energy status in the cell. Furthermore its interaction with the p53 pathway highlights its role in controlling cell survival and the response to DNA damage.
- Associated diseases and disorders
HBXIP displays connections to cancer and viral infections. In cancer HBXIP overexpression correlates with tumor progression and poor patient prognosis commonly seen in breast cancer. Through its influence on nuclear factor-kB (NF-kB) HBXIP associates with inflammatory responses tied to cancer progression. Additionally its relationship with hepatitis B viral oncogenesis highlights a link between HBXIP and liver disorders signaling interaction with the viral HBx protein as a point of mechanism.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-HBXIP antibody [EPR11531(B)] - BSA and Azide free (ab249343), expandable thumbnail](https://content.abcam.com/products/images/hbxip-antibody-epr11531b-bsa-and-azide-free-ab249343--western-blot-img56978.jpg "Western blot - Anti-HBXIP antibody [EPR11531(B)] - BSA and Azide free (ab249343)")
Western blot - Anti-HBXIP antibody [EPR11531(B)] - BSA and Azide free (ab249343)
This data was developed using Anti-HBXIP antibody [EPR11531(B)] ab157480, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-HBXIP antibody [EPR11531(B)] (Anti-HBXIP antibody [EPR11531(B)] ab157480) at 1/1000 dilution
Lane 1: BxPC-3 cell lysate at 10 µg
Lane 2: MCF7 cell lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 10 kDa
![Flow Cytometry (Intracellular) - Anti-HBXIP antibody [EPR11531(B)] - BSA and Azide free (ab249343), expandable thumbnail](https://content.abcam.com/products/images/hbxip-antibody-epr11531b-bsa-and-azide-free-ab249343--flow-cytometry-intracellular-img79264.jpg "Flow Cytometry (Intracellular) - Anti-HBXIP antibody [EPR11531(B)] - BSA and Azide free (ab249343)")
Flow Cytometry (Intracellular) - Anti-HBXIP antibody [EPR11531(B)] - BSA and Azide free (ab249343)
This data was developed using Anti-HBXIP antibody [EPR11531(B)] ab157480, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of permeabilized BxPC-3 cells labeling HBXIP with Anti-HBXIP antibody [EPR11531(B)] ab157480 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).
![Western blot - Anti-HBXIP antibody [EPR11531(B)] - BSA and Azide free (ab249343), expandable thumbnail](https://content.abcam.com/products/images/hbxip-antibody-epr11531b-bsa-and-azide-free-ab249343--western-blot-img94493.jpg "Western blot - Anti-HBXIP antibody [EPR11531(B)] - BSA and Azide free (ab249343)")
Western blot - Anti-HBXIP antibody [EPR11531(B)] - BSA and Azide free (ab249343)
This data was developed using Anti-HBXIP antibody [EPR11531(B)] ab157480, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-HBXIP antibody [EPR11531(B)] (Anti-HBXIP antibody [EPR11531(B)] ab157480) at 1/1000 dilution
All lanes: immunoprecipitation pellet from BxPC-3 cell lysate
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 10 kDa
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