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AB249343

Anti-HBXIP antibody [EPR11531(B)] - BSA and Azide free

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Rabbit Recombinant Monoclonal HBXIP antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

HBXIP, XIP, LAMTOR5, Ragulator complex protein LAMTOR5, Hepatitis B virus X-interacting protein, Late endosomal/lysosomal adaptor and MAPK and MTOR activator 5, HBV X-interacting protein, HBX-interacting protein

4 Images
Flow Cytometry (Intracellular) - Anti-HBXIP antibody [EPR11531(B)] - BSA and Azide free (AB249343)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-HBXIP antibody [EPR11531(B)] - BSA and Azide free (AB249343)

This data was developed using ab157480, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized BxPC-3 cells labeling HBXIP with ab157480 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).

Western blot - Anti-HBXIP antibody [EPR11531(B)] - BSA and Azide free (AB249343)
  • WB

Lab

Western blot - Anti-HBXIP antibody [EPR11531(B)] - BSA and Azide free (AB249343)

This data was developed using ab157480, the same antibody clone in a different buffer formulation.

Western blot : Anti-HBXIP antibody [EPR11531(B)] ab157480 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 9 kDa in Wild-type A549 cell lysates with no signal observed at this size in LAMTOR5 knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-HBXIP antibody [EPR11531(B)] (<a href='/en-us/products/primary-antibodies/hbxip-antibody-epr11531b-ab157480'>ab157480</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

LAMTOR5 knockout A549 at 20 µg

Lane 3:

PC-3 at 20 µg

Lane 4:

MCF7 at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 10 kDa

Observed band size: 9 kDa,37 kDa

false

Western blot - Anti-HBXIP antibody [EPR11531(B)] - BSA and Azide free (AB249343)
  • WB

Unknown

Western blot - Anti-HBXIP antibody [EPR11531(B)] - BSA and Azide free (AB249343)

This data was developed using ab157480, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-HBXIP antibody [EPR11531(B)] (<a href='/en-us/products/primary-antibodies/hbxip-antibody-epr11531b-ab157480'>ab157480</a>) at 1/1000 dilution

Lane 1:

BxPC-3 cell lysate at 10 µg

Lane 2:

MCF7 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 10 kDa

false

Western blot - Anti-HBXIP antibody [EPR11531(B)] - BSA and Azide free (AB249343)
  • WB

Unknown

Western blot - Anti-HBXIP antibody [EPR11531(B)] - BSA and Azide free (AB249343)

This data was developed using ab157480, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-HBXIP antibody [EPR11531(B)] (<a href='/en-us/products/primary-antibodies/hbxip-antibody-epr11531b-ab157480'>ab157480</a>) at 1/1000 dilution

All lanes:

immunoprecipitation pellet from BxPC-3 cell lysate

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 10 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11531(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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  • 4
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab249343 is the carrier-free version of ab157480.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HBXIP also known as Hepatitis B X-interacting protein or LAMTOR5 is a protein with a mass of approximately 18 kDa. HBXIP binds to the HIV-1 protease and is involved in the activation of diverse cellular processes. It predominantly expresses in heart kidney liver and skeletal muscle tissues. The protein's mechanical role involves interacting with the hepatitis B virus X protein (HBx) enhancing the replication of hepatitis B virus particles.
Biological function summary

HBXIP impacts multiple cellular functions in a complex manner. It participates in the regulation of centrosome dynamics and cell division. HBXIP interacts with proteins such as survivin in a complex that plays a role in mitosis. HBXIP also influences gene expression by modulating estrogen receptor signaling. These functions make HBXIP a significant component in cellular growth control.

Pathways

HBXIP engages in cell cycle regulation and apoptosis. It affects the mTOR signaling pathway important for cell growth and metabolism. Within this context HBXIP acts alongside proteins like mTOR and RHEB influencing nutrient and energy status in the cell. Furthermore its interaction with the p53 pathway highlights its role in controlling cell survival and the response to DNA damage.

HBXIP displays connections to cancer and viral infections. In cancer HBXIP overexpression correlates with tumor progression and poor patient prognosis commonly seen in breast cancer. Through its influence on nuclear factor-kB (NF-kB) HBXIP associates with inflammatory responses tied to cancer progression. Additionally its relationship with hepatitis B viral oncogenesis highlights a link between HBXIP and liver disorders signaling interaction with the viral HBx protein as a point of mechanism.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

As part of the Ragulator complex it is involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids (PubMed : 22980980, PubMed : 29158492, PubMed : 30181260). Activated by amino acids through a mechanism involving the lysosomal V-ATPase, the Ragulator plays a dual role for the small GTPases Rag (RagA/RRAGA, RagB/RRAGB, RagC/RRAGC and/or RagD/RRAGD) : it (1) acts as a guanine nucleotide exchange factor (GEF), activating the small GTPases Rag and (2) mediates recruitment of Rag GTPases to the lysosome membrane (PubMed : 22980980, PubMed : 28935770, PubMed : 29107538, PubMed : 29158492, PubMed : 30181260). Activated Ragulator and Rag GTPases function as a scaffold recruiting mTORC1 to lysosomes where it is in turn activated (PubMed : 22980980, PubMed : 29158492, PubMed : 30181260). When complexed to BIRC5, interferes with apoptosome assembly, preventing recruitment of pro-caspase-9 to oligomerized APAF1, thereby selectively suppressing apoptosis initiated via the mitochondrial/cytochrome c pathway (PubMed : 12773388).
See full target information LAMTOR5
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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