AB232859

Anti-HC-II antibody

Name: Anti-HC-II antibody (ab232859) | Abcam
Brand: Abcam Limited
SKU: AB232859

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(2 Publications)

Rabbit Polyclonal HC-II antibody. Suitable for WB, IHC-P and reacts with Human, Pig samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SERPIND1 aa 250 to C-terminus.

View Alternative Names

HCF2, SERPIND1, Heparin cofactor 2, Heparin cofactor II, Protease inhibitor leuserpin-2, Serpin D1, HC-II, HLS2

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HC-II antibody (AB232859)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HC-II antibody (AB232859)

Formalin-fixed, paraffin-embedded human liver tissue stained for HC-II with ab232859 at 30 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HC-II antibody (AB232859)

Formalin-fixed, paraffin-embedded human liver tissue stained for HC-II with ab232859 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-HC-II antibody (AB232859)

All lanes:

Western blot - Anti-HC-II antibody (ab232859) at 1 µg/mL

Lane 1:

Human serum

Lane 2:

Pig lung tissue lysate

Lane 3:

Pig liver tissue lysate

Lane 4:

Pig kidney tissue lysate

Secondary

All lanes:

HRP-linked Guinea pig anti-Rabbit at 1/1000 dilution

Predicted band size: 57 kDa

false

Supplier Data

Western blot - Anti-HC-II antibody (AB232859)

All lanes:

Western blot - Anti-HC-II antibody (ab232859) at 2 µg/mL

All lanes:

Recombinant human HC-II protein

Predicted band size: 57 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Pig

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human SERPIND1 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P05546

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HC-II also known as heparin cofactor II is an enzyme that acts as a serine protease inhibitor. It mainly inhibits thrombin playing a significant role in the regulation of coagulation. HC-II has an approximate molecular mass of 65 kDa and is primarily expressed in the liver and circulates in plasma. The HC protein is similar to another anticoagulant protein antithrombin but is specifically activated by dermatan sulfate.

Biological function summary

Heparin cofactor II plays an essential role in maintaining hemostatic balance. It does not function as part of a larger complex but individually regulates thrombin activity. HC-II inhibits fibrin formation and platelet aggregation controlling thrombin’s role in the coagulation cascade. The activity of HC-II is important for keeping blood clot formation under control preventing excessive clot formation which can lead to vascular diseases.

Pathways

Heparin cofactor II works in the coagulation pathway where it closely interacts with thrombin. Dermatan sulfate enhances its inhibitory effects on thrombin distinct from the action of heparin on antithrombin. Its regulation of thrombin makes it an important component in the hemostatic pathway maintaining normal circulation by balancing blood clotting and bleeding.

Heparin cofactor II is associated with thrombotic disorders including venous thromboembolism. Deficiency or malfunctioning of HC-II can lead to an increased risk of thrombosis due to inadequate inhibition of thrombin. Besides HC-II is associated with atherosclerosis where its interaction with dermatan sulfate could influence the progression of vascular lesions. The protein's role in modulating thrombin activity further connects it to coagulation factor V which can also impact thrombotic disease processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Thrombin inhibitor activated by the glycosaminoglycans, heparin or dermatan sulfate. In the presence of the latter, HC-II becomes the predominant thrombin inhibitor in place of antithrombin III (AT-III). Also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner.. Peptides at the N-terminal of HC-II have chemotactic activity for both monocytes and neutrophils.. Isoform 2. Shows negligible inhibition, in vitro, of thrombin and tPA and no inhibition of factor Xa, in vitro.

See full target information SERPIND1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 9:e21616 PubMed38027820

2023

Secreted proteins in plasma and placenta as novel non-invasive biomarkers for intrahepatic cholestasis of pregnancy: A case-control study.

Applications

Unspecified application

Species

Unspecified reactive species

Lingyan Chen,Jingyang Li,Yilan You,Zhiwen Qian,Jiayu Liu,Ying Jiang,Ying Gu,Jianping Xiao,Yan Zhang

Fundamental research 5:1804-1821 PubMed40777809

2023

Stem cell-derived small extracellular vesicles containing miR-27b-3p attenuated osteoarthritis through inhibition of leukaemia inhibitory factor.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoguang Zhang,Wei Chen,Shenghui Lan,Yuxiang Hu,Hongxin Pei,Zhili He,Zhipeng Dai,Yulong Wei,Zhenxing Wang,Qinyu Ma,Fenfei Zhao,Juan Wang,Zengwu Shao,Yong Liu,Shuhua Yang,Hongtao Tian,Wei Tong

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-HC-II antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HC-II antibody (AB232859)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HC-II antibody (AB232859)

Western blot - Anti-HC-II antibody (AB232859)

Western blot - Anti-HC-II antibody (AB232859)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise