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Rabbit Recombinant Monoclonal HC-II antibody. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples.

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Images

Immunoprecipitation - Anti-HC-II antibody [EPR25618-1] (AB289971), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-HC-II antibody [EPR25618-1] (AB289971), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-HC-II antibody [EPR25618-1] (AB289971), expandable thumbnail
  • Western blot - Anti-HC-II antibody [EPR25618-1] (AB289971), expandable thumbnail
  • Western blot - Anti-HC-II antibody [EPR25618-1] (AB289971), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBIHC-PFlow Cyt (Intra)
Human
Tested
Tested
Tested
Not recommended
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

1/50

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/30

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Target data

Function

Thrombin inhibitor activated by the glycosaminoglycans, heparin or dermatan sulfate. In the presence of the latter, HC-II becomes the predominant thrombin inhibitor in place of antithrombin III (AT-III). Also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner.Peptides at the N-terminal of HC-II have chemotactic activity for both monocytes and neutrophils.

Alternative names

Heparin cofactor 2, Heparin cofactor II, Protease inhibitor leuserpin-2, Serpin D1, HC-II, HLS2, HCF2, SERPIND1

Recommended products

Rabbit Recombinant Monoclonal HC-II antibody. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR25618-1

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

HC-II also known as heparin cofactor II is an enzyme that acts as a serine protease inhibitor. It mainly inhibits thrombin playing a significant role in the regulation of coagulation. HC-II has an approximate molecular mass of 65 kDa and is primarily expressed in the liver and circulates in plasma. The HC protein is similar to another anticoagulant protein antithrombin but is specifically activated by dermatan sulfate.

Biological function summary

Heparin cofactor II plays an essential role in maintaining hemostatic balance. It does not function as part of a larger complex but individually regulates thrombin activity. HC-II inhibits fibrin formation and platelet aggregation controlling thrombin’s role in the coagulation cascade. The activity of HC-II is important for keeping blood clot formation under control preventing excessive clot formation which can lead to vascular diseases.

Pathways

Heparin cofactor II works in the coagulation pathway where it closely interacts with thrombin. Dermatan sulfate enhances its inhibitory effects on thrombin distinct from the action of heparin on antithrombin. Its regulation of thrombin makes it an important component in the hemostatic pathway maintaining normal circulation by balancing blood clotting and bleeding.

Associated diseases and disorders

Heparin cofactor II is associated with thrombotic disorders including venous thromboembolism. Deficiency or malfunctioning of HC-II can lead to an increased risk of thrombosis due to inadequate inhibition of thrombin. Besides HC-II is associated with atherosclerosis where its interaction with dermatan sulfate could influence the progression of vascular lesions. The protein's role in modulating thrombin activity further connects it to coagulation factor V which can also impact thrombotic disease processes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunoprecipitation - Anti-HC-II antibody [EPR25618-1] (ab289971), expandable thumbnail

    Immunoprecipitation - Anti-HC-II antibody [EPR25618-1] (ab289971)

    HC-II was immunoprecipitated from HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate with ab289971 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab289971 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate 10 ug

    Lane 2: ab289971 IP in HepG2 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab289971 in HepG2 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds

    All lanes: Immunoprecipitation - Anti-HC-II antibody [EPR25618-1] (ab289971)

    Predicted band size: 57 kDa

    Observed band size: 66-75 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-HC-II antibody [EPR25618-1] (ab289971), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-HC-II antibody [EPR25618-1] (ab289971)

    Immunofluorescent analysis of 100% Methanol-fixed, 0.1% TritonX-100 permebilized HepG2 cells labelling HC-II with ab289971 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in HepG2 cell line. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

  • Flow Cytometry (Intracellular) - Anti-HC-II antibody [EPR25618-1] (ab289971), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-HC-II antibody [EPR25618-1] (ab289971)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HepG2 (Human hepatocellular carcinoma epithelial cell) cells labelling HC-II with ab289971 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-HC-II antibody [EPR25618-1] (ab289971), expandable thumbnail

    Western blot - Anti-HC-II antibody [EPR25618-1] (ab289971)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 15 seconds

    All lanes: Western blot - Anti-HC-II antibody [EPR25618-1] (ab289971) at 1/1000 dilution

    Lane 1: Human liver tissue lysate(RIPA) at 20 µg

    Lane 2: Human liver tissue lysate(HOT) at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 57 kDa

    Observed band size: 66-75 kDa

  • Western blot - Anti-HC-II antibody [EPR25618-1] (ab289971), expandable thumbnail

    Western blot - Anti-HC-II antibody [EPR25618-1] (ab289971)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    All these lysates are RIPA buffer.

    Exposure time: 59 seconds

    All lanes: Western blot - Anti-HC-II antibody [EPR25618-1] (ab289971) at 1/1000 dilution

    Lane 1: HepG2 (human hepatocellar carcinoma epithelial cell) transfected with scrambled siRNA control, whole cell lysate at 20 µg

    Lane 2: HepG2 transfected with HC-II siRNA, whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 57 kDa

    Observed band size: 66-75 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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