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AB316348

Anti-hCAP-D3 antibody [2B5]

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Mouse Monoclonal hCAP-D3 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human NCAPD3.

View Alternative Names

CAPD3, KIAA0056, NCAPD3, Condensin-2 complex subunit D3, Non-SMC condensin II complex subunit D3, hCAP-D3

1 Images
Western blot - Anti-hCAP-D3 Antibody [2B5] (AB316348)
  • WB

Supplier Data

Western blot - Anti-hCAP-D3 Antibody [2B5] (AB316348)

Western blot of HeLa cell lysate with ab316348. Antibody used at 2.5ug/ml, detection with anti-mouse Fc HRP conjugate, with 90 second ECL exposure. A protein band at approximately 170-180kD is seen, consistent with the expected MW of NCAPD3.

All lanes:

Western blot - Anti-hCAP-D3 Antibody [2B5] (ab316348) at 2.5 µg/mL

All lanes:

HeLa cell lysate

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2B5

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human NCAPD3.

P42695

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/400", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Storage buffer
Preservative: 0.1% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1 month
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HCAP-D3 also known as chromosome-associated protein D3 or CAP-D3 plays a role in the regulation of chromatid structure during cell division. With a molecular mass of approximately 160 kDa this protein is a component of the condensin II complex. hCAP-D3 is expressed in various tissues throughout the body prominently in cells that are dividing. Its expression changes can influence chromosomal segregation which is essential for proper cell cycle progression.
Biological function summary

HCAP-D3 influences the stabilization of chromosome architecture. It is a part of the condensin II complex working with other proteins like hCAP-H2 and hCAP-G2 to facilitate mitotic chromatid condensation. The correct function of hCAP-D3 during mitosis ensures proper separation of sister chromatids maintaining genomic integrity. The protein's mechanic role is important in preventing aneuploidy which can lead to various cellular malfunctions.

Pathways

HCAP-D3 participates in the mitotic cell cycle pathway significantly affecting chromosomal condensation processes. Its interaction with proteins like SMC2 and SMC4 is critical for the optimal function of the condensin II complex. Additionally hCAP-D3 engages in pathways associated with DNA repair and replication highlighting its importance in cell division and genomic stability. These interactions demonstrate its integration within cellular processes that preserve cell health.

HCAP-D3 associates with certain cancers due to its role in genomic stability. Aberrations in hCAP-D3 expression or function can lead to improper chromosomal segregation contributing to oncogenesis. Specifically research links hCAP-D3 to colorectal cancer where its altered activity may drive tumor progression. The protein also has connections to proteins involved in the control of mitotic checkpoints such as Aurora B highlighting its relevance in maintaining cell cycle fidelity and preventing disease.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulatory subunit of the condensin-2 complex, a complex which establishes mitotic chromosome architecture and is involved in physical rigidity of the chromatid axis (PubMed : 14532007). May promote the resolution of double-strand DNA catenanes (intertwines) between sister chromatids. Condensin-mediated compaction likely increases tension in catenated sister chromatids, providing directionality for type II topoisomerase-mediated strand exchanges toward chromatid decatenation. Specifically required for decatenation of centromeric ultrafine DNA bridges during anaphase. Early in neurogenesis, may play an essential role to ensure accurate mitotic chromosome condensation in neuron stem cells, ultimately affecting neuron pool and cortex size (PubMed : 27737959).
See full target information NCAPD3
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Product promise

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