Rabbit Polyclonal hCAP-D3 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human NCAPD3.
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Publications
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- Nature communications 13:14742022A point mutation in HIV-1 integrase redirects proviral integration into centromeric repeats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShelby Winans,Hyun Jae Yu,Kenia de Los Santos,Gary Z Wang,Vineet N KewalRamani,Stephen P GoffPubMed 35304442
- Genome biology 23:702022Meiosis-specific cohesin complexes display essential and distinct roles in mitotic embryonic stem cell chromosomes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEui-Hwan Choi,Seobin Yoon,Young Eun Koh,Tae Kyung Hong,Jeong Tae Do,Bum-Kyu Lee,Yoonsoo Hahn,Keun P KimPubMed 35241136
- The Journal of cell biology 219:2019Aurora A promotes chromosome congression by activating the condensin-dependent pool of KIF4A.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElena Poser,Renaud Caous,Ulrike Gruneberg,Francis A BarrPubMed 31881080
- Nature cell biology 21:1393-14022019A chromosome folding intermediate at the condensin-to-cohesin transition during telophase.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKristin Abramo et. al.PubMed 31685986
- Cancers 11:2019Diminished Condensin Gene Expression Drives Chromosome Instability That May Contribute to Colorectal Cancer Pathogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAllison K Baergen et. al.PubMed 31357676
- Journal of cellular physiology 234:20755-207682019Condensin II subunit NCAPH2 associates with shelterin protein TRF1 and is required for telomere stability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHeather A Wallace et. al.PubMed 31026066
- Cell cycle (Georgetown, Tex.) 14:2160-702015A novel role for the condensin II complex in cellular senescence.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuhki Yokoyama,Hengrui Zhu,Rugang Zhang,Ken-ichi NomaPubMed 26017022
- Molecular cell 40:22-332010CRL4(Cdt2)-mediated destruction of the histone methyltransferase Set8 prevents premature chromatin compaction in S phase.Applications:WBReactive species:HumanRichard C Centore,Courtney G Havens,Amity L Manning,Ju-Mei Li,Rachel Litman Flynn,Alice Tse,Jianping Jin,Nicholas J Dyson,Johannes C Walter,Lee ZouPubMed 20932472
- Nature 466:508-122010PHF8 mediates histone H4 lysine 20 demethylation events involved in cell cycle progression.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human NCAPD3. The exact immunogen used to generate this antibody is proprietary information. Database link P42695
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Chimpanzee | Predicted | Predicted |
| Gorilla | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee, Rhesus monkey, Gorilla | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee, Rhesus monkey, Gorilla | Dilution info - | Notes - |
Target data
Function
Regulatory subunit of the condensin-2 complex, a complex which establishes mitotic chromosome architecture and is involved in physical rigidity of the chromatid axis (PubMed:14532007). May promote the resolution of double-strand DNA catenanes (intertwines) between sister chromatids. Condensin-mediated compaction likely increases tension in catenated sister chromatids, providing directionality for type II topoisomerase-mediated strand exchanges toward chromatid decatenation. Specifically required for decatenation of centromeric ultrafine DNA bridges during anaphase. Early in neurogenesis, may play an essential role to ensure accurate mitotic chromosome condensation in neuron stem cells, ultimately affecting neuron pool and cortex size (PubMed:27737959).
Alternative names
CAPD3, KIAA0056, NCAPD3, Condensin-2 complex subunit D3, Non-SMC condensin II complex subunit D3, hCAP-D3
Recommended products
Rabbit Polyclonal hCAP-D3 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human NCAPD3.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human NCAPD3. The exact immunogen used to generate this antibody is proprietary information. Database link P42695
- Purification technique
- Affinity purification Immunogen
- Specificity
We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
HCAP-D3 also known as chromosome-associated protein D3 or CAP-D3 plays a role in the regulation of chromatid structure during cell division. With a molecular mass of approximately 160 kDa this protein is a component of the condensin II complex. hCAP-D3 is expressed in various tissues throughout the body prominently in cells that are dividing. Its expression changes can influence chromosomal segregation which is essential for proper cell cycle progression.
- Biological function summary
HCAP-D3 influences the stabilization of chromosome architecture. It is a part of the condensin II complex working with other proteins like hCAP-H2 and hCAP-G2 to facilitate mitotic chromatid condensation. The correct function of hCAP-D3 during mitosis ensures proper separation of sister chromatids maintaining genomic integrity. The protein's mechanic role is important in preventing aneuploidy which can lead to various cellular malfunctions.
- Pathways
HCAP-D3 participates in the mitotic cell cycle pathway significantly affecting chromosomal condensation processes. Its interaction with proteins like SMC2 and SMC4 is critical for the optimal function of the condensin II complex. Additionally hCAP-D3 engages in pathways associated with DNA repair and replication highlighting its importance in cell division and genomic stability. These interactions demonstrate its integration within cellular processes that preserve cell health.
- Associated diseases and disorders
HCAP-D3 associates with certain cancers due to its role in genomic stability. Aberrations in hCAP-D3 expression or function can lead to improper chromosomal segregation contributing to oncogenesis. Specifically research links hCAP-D3 to colorectal cancer where its altered activity may drive tumor progression. The protein also has connections to proteins involved in the control of mitotic checkpoints such as Aurora B highlighting its relevance in maintaining cell cycle fidelity and preventing disease.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunoprecipitation - Anti-hCAP-D3 antibody (ab70349)
All lanes:200µg Hela whole cell lysate and ab70349 at 1µg/ml.
Lane 1: ab70349 at 3 µg/mg lysate for IP.
Lane 2: IgG control.All lanes: Immunoprecipitation - Anti-hCAP-D3 antibody (ab70349)
Predicted band size: 169 kDa
Western blot - Anti-hCAP-D3 antibody (ab70349)
All lanes: Western blot - Anti-hCAP-D3 antibody (ab70349) at 0.04 µg/mL
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: HeLa whole cell lysate at 15 µg
Lane 3: HeLa whole cell lysate at 5 µg
Lane 4: 293T whole cell lysate at 50 µg
Predicted band size: 169 kDa
Observed band size: 174 kDa
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