AB70349

Anti-hCAP-D3 antibody

Name: Anti-hCAP-D3 antibody (ab70349) | Abcam
Brand: Abcam Limited
SKU: AB70349
Price: 535 USD
Availability: InStock

(1 Review)

(9 Publications)

Rabbit Polyclonal hCAP-D3 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human NCAPD3.

View Alternative Names

CAPD3, KIAA0056, NCAPD3, Condensin-2 complex subunit D3, Non-SMC condensin II complex subunit D3, hCAP-D3

2 Images

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Immunoprecipitation - Anti-hCAP-D3 antibody (AB70349)

All lanes : 200µg Hela whole cell lysate and ab70349 at 1µg/ml. Lane 1 : ab70349 at 3 µg/mg lysate for IP. Lane 2 : IgG control.

All lanes:

Immunoprecipitation - Anti-hCAP-D3 antibody (ab70349)

Predicted band size: 169 kDa

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Western blot - Anti-hCAP-D3 antibody (AB70349)

All lanes:

Western blot - Anti-hCAP-D3 antibody (ab70349) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 169 kDa

Observed band size: 174 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human NCAPD3. The exact immunogen used to generate this antibody is proprietary information.

P42695

Specificity

We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HCAP-D3 also known as chromosome-associated protein D3 or CAP-D3 plays a role in the regulation of chromatid structure during cell division. With a molecular mass of approximately 160 kDa this protein is a component of the condensin II complex. hCAP-D3 is expressed in various tissues throughout the body prominently in cells that are dividing. Its expression changes can influence chromosomal segregation which is essential for proper cell cycle progression.

Biological function summary

HCAP-D3 influences the stabilization of chromosome architecture. It is a part of the condensin II complex working with other proteins like hCAP-H2 and hCAP-G2 to facilitate mitotic chromatid condensation. The correct function of hCAP-D3 during mitosis ensures proper separation of sister chromatids maintaining genomic integrity. The protein's mechanic role is important in preventing aneuploidy which can lead to various cellular malfunctions.

Pathways

HCAP-D3 participates in the mitotic cell cycle pathway significantly affecting chromosomal condensation processes. Its interaction with proteins like SMC2 and SMC4 is critical for the optimal function of the condensin II complex. Additionally hCAP-D3 engages in pathways associated with DNA repair and replication highlighting its importance in cell division and genomic stability. These interactions demonstrate its integration within cellular processes that preserve cell health.

HCAP-D3 associates with certain cancers due to its role in genomic stability. Aberrations in hCAP-D3 expression or function can lead to improper chromosomal segregation contributing to oncogenesis. Specifically research links hCAP-D3 to colorectal cancer where its altered activity may drive tumor progression. The protein also has connections to proteins involved in the control of mitotic checkpoints such as Aurora B highlighting its relevance in maintaining cell cycle fidelity and preventing disease.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Regulatory subunit of the condensin-2 complex, a complex which establishes mitotic chromosome architecture and is involved in physical rigidity of the chromatid axis (PubMed : 14532007). May promote the resolution of double-strand DNA catenanes (intertwines) between sister chromatids. Condensin-mediated compaction likely increases tension in catenated sister chromatids, providing directionality for type II topoisomerase-mediated strand exchanges toward chromatid decatenation. Specifically required for decatenation of centromeric ultrafine DNA bridges during anaphase. Early in neurogenesis, may play an essential role to ensure accurate mitotic chromosome condensation in neuron stem cells, ultimately affecting neuron pool and cortex size (PubMed : 27737959).

See full target information NCAPD3

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:1474 PubMed35304442

2022

A point mutation in HIV-1 integrase redirects proviral integration into centromeric repeats.

Applications

Unspecified application

Species

Unspecified reactive species

Shelby Winans,Hyun Jae Yu,Kenia de Los Santos,Gary Z Wang,Vineet N KewalRamani,Stephen P Goff

Genome biology 23:70 PubMed35241136

2022

Meiosis-specific cohesin complexes display essential and distinct roles in mitotic embryonic stem cell chromosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Eui-Hwan Choi,Seobin Yoon,Young Eun Koh,Tae Kyung Hong,Jeong Tae Do,Bum-Kyu Lee,Yoonsoo Hahn,Keun P Kim

The Journal of cell biology 219: PubMed31881080

2019

Aurora A promotes chromosome congression by activating the condensin-dependent pool of KIF4A.

Applications

Unspecified application

Species

Unspecified reactive species

Elena Poser,Renaud Caous,Ulrike Gruneberg,Francis A Barr

Nature cell biology 21:1393-1402 PubMed31685986

2019

A chromosome folding intermediate at the condensin-to-cohesin transition during telophase.

Applications

Unspecified application

Species

Unspecified reactive species

Kristin Abramo,Anne-Laure Valton,Sergey V Venev,Hakan Ozadam,A Nicole Fox,Job Dekker

Cancers 11: PubMed31357676

2019

Diminished Condensin Gene Expression Drives Chromosome Instability That May Contribute to Colorectal Cancer Pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Allison K Baergen,Lucile M Jeusset,Zelda Lichtensztejn,Kirk J McManus

Journal of cellular physiology 234:20755-20768 PubMed31026066

2019

Condensin II subunit NCAPH2 associates with shelterin protein TRF1 and is required for telomere stability.

Applications

Unspecified application

Species

Unspecified reactive species

Heather A Wallace,Vibhuti Rana,Huy Q Nguyen,Giovanni Bosco

Cell cycle (Georgetown, Tex.) 14:2160-70 PubMed26017022

2015

A novel role for the condensin II complex in cellular senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Yuhki Yokoyama,Hengrui Zhu,Rugang Zhang,Ken-ichi Noma

Molecular cell 40:22-33 PubMed20932472

2010

CRL4(Cdt2)-mediated destruction of the histone methyltransferase Set8 prevents premature chromatin compaction in S phase.

Applications

Species

Human

Richard C Centore,Courtney G Havens,Amity L Manning,Ju-Mei Li,Rachel Litman Flynn,Alice Tse,Jianping Jin,Nicholas J Dyson,Johannes C Walter,Lee Zou

Nature 466:508-12 PubMed20622854

2010

PHF8 mediates histone H4 lysine 20 demethylation events involved in cell cycle progression.

Applications

Species

Human

Wen Liu,Bogdan Tanasa,Oksana V Tyurina,Tian Yuan Zhou,Reto Gassmann,Wei Ting Liu,Kenneth A Ohgi,Chris Benner,Ivan Garcia-Bassets,Aneel K Aggarwal,Arshad Desai,Pieter C Dorrestein,Christopher K Glass,Michael G Rosenfeld

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-hCAP-D3 antibody

Immunoprecipitation - Anti-hCAP-D3 antibody (AB70349)

Western blot - Anti-hCAP-D3 antibody (AB70349)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

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