AB244254

Anti-HCC1 antibody

Name: Anti-HCC1 antibody (ab244254) | Abcam
Brand: Abcam Limited
SKU: AB244254
Price: 630 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal HCC1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RBM39 aa 400 to C-terminus.

View Alternative Names

HCC1, RNPC2, RBM39, RNA-binding protein 39, CAPER alpha, Hepatocellular carcinoma protein 1, RNA-binding motif protein 39, RNA-binding region-containing protein 2, Splicing factor HCC1, CAPERalpha

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCC1 antibody (AB244254)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCC1 antibody (AB244254)

Formalin-fixed, paraffin-embedded human cervix tissue stained for HCC1 with ab244254 at a 1/50 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCC1 antibody (AB244254)

Formalin-fixed, paraffin-embedded human testis tissue stained for HCC1 with ab244254 at a 1/50 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCC1 antibody (AB244254)

Formalin-fixed, paraffin-embedded human cerebral cortex tissue stained for HCC1 with ab244254 at a 1/50 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCC1 antibody (AB244254)

Formalin-fixed, paraffin-embedded human colon tissue stained for HCC1 with ab244254 at a 1/50 dilution in immunohistochemical analysis.

Unknown

Western blot - Anti-HCC1 antibody (AB244254)

All lanes:

Western blot - Anti-HCC1 antibody (ab244254) at 0.4 µg/mL

Lane 1:

Caco-2 (Human colorectal adenocarcinoma cell line) cells transfected with control siRNA

Lane 2:

Caco-2 cells transfected with siRNA#1

Predicted band size: 59 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human RBM39 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q14498

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target HCC1 also known as HkCdc4 is a protein with a molecular mass of approximately 44 kDa. It plays a role in cell cycle regulation by acting as a regulator of cyclin levels. HCC1 is widely expressed in numerous tissues with notable expression in testis colon and leukocytes. It functions primarily within the nucleus where it impacts various cellular processes including transcription and mitosis.

Biological function summary

HCC1 influences cell division and growth regulation as part of a larger protein complex. It interacts with components such as cyclins and CDKs to ensure proper progression through the cell cycle. The protein modulates the stability and degradation of specific cyclins therefore contributing to orderly cell cycle transitions. These activities position HCC1 as an important player in maintaining chromosomal stability further integrating it with mitotic checkpoint machinery.

Pathways

The interaction of HCC1 integrates into pivotal pathways like the cell cycle regulatory pathway and the Wnt signaling pathway. The cell cycle pathway involves interactions with CDKs and cyclins affecting checkpoints and transitions. In the Wnt signaling context HCC1 interacts with proteins like β-catenin helping to harmonize cellular proliferation signals and the cell cycle's momentum. These interactions indicate the adaptability of HCC1 within complex pathway networks essential for cellular proliferation and signaling.

Aberrant HCC1 activity links to various types of cancer and reproductive disorders. HCC1 dysfunction often associates with hepatocellular carcinoma due to its role in cell cycle misregulation. This dysfunction also relates to disorders such as testicular dysgenesis syndrome where abnormal expression of HCC1 may contribute to improper developmental signaling. In these disorders HCC1 may work in conjunction with proteins like p53 further justifying its influence in processes that regulate cell growth and apoptosis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

RNA-binding protein that acts as a pre-mRNA splicing factor (PubMed : 15694343, PubMed : 24795046, PubMed : 28302793, PubMed : 28437394, PubMed : 31271494). Acts by promoting exon inclusion via regulation of exon cassette splicing (PubMed : 31271494). Also acts as a transcriptional coactivator for steroid nuclear receptors ESR1/ER-alpha and ESR2/ER-beta, and JUN/AP-1, independently of the pre-mRNA splicing factor activity (By similarity).

See full target information RBM39

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2304164 PubMed37715297

2023

A Smart Intracellular Self-Assembling Bioorthogonal Raman Active Nanoprobe for Targeted Tumor Imaging.

Applications

Unspecified application

Species

Unspecified reactive species

Swati Tanwar,Behnaz Ghaemi,Piyush Raj,Aruna Singh,Lintong Wu,Yue Yuan,Dian R Arifin,Michael T McMahon,Jeff W M Bulte,Ishan Barman

American journal of cancer research 13:2922-2937 PubMed37559979

2023

Indisulam exerts anticancer effects via modulation of transcription, translation and alternative splicing on human cervical cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhihui Dou,Xuetian Zhang,Wei Su,Taotao Zhang,Fei Ye,Dapeng Zhao,Xiaohua Chen,Qiang Li,Hong Zhang,Cuixia Di

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HCC1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCC1 antibody (AB244254)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCC1 antibody (AB244254)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCC1 antibody (AB244254)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCC1 antibody (AB244254)

Western blot - Anti-HCC1 antibody (AB244254)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise