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AB224321

Anti-HCCS antibody

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(1 Publication)

Rabbit Polyclonal HCCS antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human HCCS aa 50-250.

View Alternative Names

CCHL, HCCS, Holocytochrome c-type synthase, Cytochrome c-type heme lyase

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCCS antibody (AB224321)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCCS antibody (AB224321)

Paraffin-embedded human oral mucosa tissue stained for HCCS using ab224321 at 1/20 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-HCCS antibody (AB224321)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HCCS antibody (AB224321)

PFA-fixed/Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for HCCS (green) using ab224321 at 4 μg/ml in ICC/IF.

Western blot - Anti-HCCS antibody (AB224321)
  • WB

Supplier Data

Western blot - Anti-HCCS antibody (AB224321)

All lanes:

Western blot - Anti-HCCS antibody (ab224321) at 1/100 dilution

Lane 1:

NIH/3T3 (mouse embryonic fibroblast cell line) cell lysate

Lane 2:

NBT-II cell lysate

Predicted band size: 31 kDa

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Western blot - Anti-HCCS antibody (AB224321)
  • WB

Supplier Data

Western blot - Anti-HCCS antibody (AB224321)

All lanes:

Western blot - Anti-HCCS antibody (ab224321) at 1/100 dilution

All lanes:

RT4 (human urinary bladder cancer cell line) cell lysate

Predicted band size: 31 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human HCCS aa 50-250. The exact immunogen used to generate this antibody is proprietary information.

P53701

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The holocytochrome c-type synthase (HCCS) also known as cytochrome c heme lyase attaches to the inner mitochondrial membrane. It catalyzes the covalent attachment of heme to apocytochrome c providing an essential step in cytochrome c maturation. HCCS with a mass around 30 kDa expresses predominantly in tissues with high energy demand such as heart and muscle tissue. The protein’s location suggests its significant role in aerobic energy production processes.
Biological function summary

Holocytochrome c-type synthase facilitates proper electron transport chain function by enabling cytochrome c activity. It operates as part of a larger mitochondrial complex critical for oxidative phosphorylation. Without HCCS the conversion of apocytochrome c to its holo form fails impacting the electron transport chain's efficiency and leading to impaired energy production.

Pathways

Holocytochrome c-type synthase plays an important role in the mitochondrial electron transport chain and apoptotic pathways. Through its function HCCS influences both the generation of ATP and regulation of programmed cell death. It interacts closely with cytochrome c an essential component in these pathways emphasizing its integrative role in cellular respiration and apoptosis regulation.

Disturbances in HCCS activity link to disorders such as microphthalmia with linear skin defects syndrome (MLS) and certain mitochondrial abnormalities. These involve alterations in the mitochondrial functionalities where HCCS interacts with cytochrome c and related proteins. Such relationships highlight the critical impact of HCCS on cellular health and stress the importance of its proper functioning in preventing disease.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Lyase that catalyzes the covalent linking of the heme group to the cytochrome C apoprotein to produce the mature functional cytochrome.
See full target information HCCS
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Theranostics 12:910-928 PubMed34976220

2022

Dysregulation of interaction between LOX fibroblast and smooth muscle cells contributes to the pathogenesis of aortic dissection.

Applications

Unspecified application

Species

Unspecified reactive species

Yinan Chen,Tao Zhang,Fang Yao,Xiang Gao,Dandan Li,Shufang Fu,Lin Mao,Fei Liu,Xuelin Zhang,Yongle Xu,Jianqing Deng,Weihao Li,Guangpu Fan,Cangsong Xiao,Yu Chen,Li Wang,Wei Guo,Bingying Zhou
View all publications
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Product promise

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