Rabbit Polyclonal HCCS antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human HCCS aa 50-250.
IgG
Rabbit
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
IHC-P | WB | ICC/IF | |
---|---|---|---|
Human | Tested | Tested | Tested |
Mouse | Expected | Tested | Expected |
Rat | Expected | Tested | Expected |
Cow | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/20.00000 - 1/50.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Cow | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species Rat | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Cow | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Cow | Dilution info - | Notes - |
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Lyase that catalyzes the covalent linking of the heme group to the cytochrome C apoprotein to produce the mature functional cytochrome.
CCHL, HCCS, CCHL, Holocytochrome c-type synthase, Cytochrome c-type heme lyase
Rabbit Polyclonal HCCS antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human HCCS aa 50-250.
IgG
Rabbit
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
The holocytochrome c-type synthase (HCCS) also known as cytochrome c heme lyase attaches to the inner mitochondrial membrane. It catalyzes the covalent attachment of heme to apocytochrome c providing an essential step in cytochrome c maturation. HCCS with a mass around 30 kDa expresses predominantly in tissues with high energy demand such as heart and muscle tissue. The protein’s location suggests its significant role in aerobic energy production processes.
Holocytochrome c-type synthase facilitates proper electron transport chain function by enabling cytochrome c activity. It operates as part of a larger mitochondrial complex critical for oxidative phosphorylation. Without HCCS the conversion of apocytochrome c to its holo form fails impacting the electron transport chain's efficiency and leading to impaired energy production.
Holocytochrome c-type synthase plays an important role in the mitochondrial electron transport chain and apoptotic pathways. Through its function HCCS influences both the generation of ATP and regulation of programmed cell death. It interacts closely with cytochrome c an essential component in these pathways emphasizing its integrative role in cellular respiration and apoptosis regulation.
Disturbances in HCCS activity link to disorders such as microphthalmia with linear skin defects syndrome (MLS) and certain mitochondrial abnormalities. These involve alterations in the mitochondrial functionalities where HCCS interacts with cytochrome c and related proteins. Such relationships highlight the critical impact of HCCS on cellular health and stress the importance of its proper functioning in preventing disease.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Terms & Conditions.
All lanes: Western blot - Anti-HCCS antibody (ab224321) at 1/100 dilution
All lanes: RT4 (human urinary bladder cancer cell line) cell lysate
Developed using the ECL technique.
Predicted band size: 31 kDa
PFA-fixed/Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for HCCS (green) using ab224321 at 4 μg/ml in ICC/IF.
Paraffin-embedded human oral mucosa tissue stained for HCCS using ab224321 at 1/20 dilution in immunohistochemical analysis.
All lanes: Western blot - Anti-HCCS antibody (ab224321) at 1/100 dilution
Lane 1: NIH/3T3 (mouse embryonic fibroblast cell line) cell lysate
Lane 2: NBT-II cell lysate
Developed using the ECL technique.
Predicted band size: 31 kDa
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