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Rabbit Polyclonal HCCS antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human HCCS aa 50-250.

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Images

Western blot - Anti-HCCS antibody (AB224321), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-HCCS antibody (AB224321), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCCS antibody (AB224321), expandable thumbnail
  • Western blot - Anti-HCCS antibody (AB224321), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human HCCS aa 50-250. The exact immunogen used to generate this antibody is proprietary information. Database link P53701

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Rat
Expected
Tested
Expected
Cow
Predicted
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/20.00000 - 1/50.00000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Cow

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

0.04000-0.40000 µg/mL

Notes

-

Species

Rat

Dilution info

0.04000-0.40000 µg/mL

Notes

-

Species

Human

Dilution info

0.04000-0.40000 µg/mL

Notes

-

Predicted
Predicted

Species

Cow

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

0.25000-2.00000 µg/mL

Notes

Fixation/Permeabilization: PFA/Triton X-100.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Cow

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Lyase that catalyzes the covalent linking of the heme group to the cytochrome C apoprotein to produce the mature functional cytochrome.

Alternative names

Recommended products

Rabbit Polyclonal HCCS antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human HCCS aa 50-250.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human HCCS aa 50-250. The exact immunogen used to generate this antibody is proprietary information. Database link P53701
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The holocytochrome c-type synthase (HCCS) also known as cytochrome c heme lyase attaches to the inner mitochondrial membrane. It catalyzes the covalent attachment of heme to apocytochrome c providing an essential step in cytochrome c maturation. HCCS with a mass around 30 kDa expresses predominantly in tissues with high energy demand such as heart and muscle tissue. The protein’s location suggests its significant role in aerobic energy production processes.

Biological function summary

Holocytochrome c-type synthase facilitates proper electron transport chain function by enabling cytochrome c activity. It operates as part of a larger mitochondrial complex critical for oxidative phosphorylation. Without HCCS the conversion of apocytochrome c to its holo form fails impacting the electron transport chain's efficiency and leading to impaired energy production.

Pathways

Holocytochrome c-type synthase plays an important role in the mitochondrial electron transport chain and apoptotic pathways. Through its function HCCS influences both the generation of ATP and regulation of programmed cell death. It interacts closely with cytochrome c an essential component in these pathways emphasizing its integrative role in cellular respiration and apoptosis regulation.

Associated diseases and disorders

Disturbances in HCCS activity link to disorders such as microphthalmia with linear skin defects syndrome (MLS) and certain mitochondrial abnormalities. These involve alterations in the mitochondrial functionalities where HCCS interacts with cytochrome c and related proteins. Such relationships highlight the critical impact of HCCS on cellular health and stress the importance of its proper functioning in preventing disease.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-HCCS antibody (ab224321), expandable thumbnail

    Western blot - Anti-HCCS antibody (ab224321)

    All lanes: Western blot - Anti-HCCS antibody (ab224321) at 1/100 dilution

    All lanes: RT4 (human urinary bladder cancer cell line) cell lysate

    Developed using the ECL technique.

    Predicted band size: 31 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-HCCS antibody (ab224321), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-HCCS antibody (ab224321)

    PFA-fixed/Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for HCCS (green) using ab224321 at 4 μg/ml in ICC/IF.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCCS antibody (ab224321), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCCS antibody (ab224321)

    Paraffin-embedded human oral mucosa tissue stained for HCCS using ab224321 at 1/20 dilution in immunohistochemical analysis.

  • Western blot - Anti-HCCS antibody (ab224321), expandable thumbnail

    Western blot - Anti-HCCS antibody (ab224321)

    All lanes: Western blot - Anti-HCCS antibody (ab224321) at 1/100 dilution

    Lane 1: NIH/3T3 (mouse embryonic fibroblast cell line) cell lysate

    Lane 2: NBT-II cell lysate

    Developed using the ECL technique.

    Predicted band size: 31 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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