AB290000

Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] - BSA and Azide free

BOND RX™ Validated
RabMAb
Recombinant
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Rabbit Recombinant Monoclonal HCF-1 / Host Cell Factor C1 antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra), IHC-P and reacts with Mouse, Human, Rat samples.

View Alternative Names

HCF1, HFC1, HCFC1, Host cell factor 1, HCF, HCF-1, C1 factor, CFF, VCAF, VP16 accessory protein

9 Images

Flow Cytometry (Intracellular) - Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] - BSA and Azide free (AB290000)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] - BSA and Azide free (AB290000)

This data was developed using ab289975, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling HCF-1 / Host Cell Factor C1 with ab289975 at 1/500 dilution (0.1μg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor^® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] - BSA and Azide free (AB290000)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] - BSA and Azide free (AB290000)

This data was developed using ab289975, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeling HCF-1 / Host Cell Factor C1 with ab289975 at 1/2000 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in HeLa cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubμle Marker (Alexa Flu^or® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] - BSA and Azide free (AB290000)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] - BSA and Azide free (AB290000)

This data was developed using ab289975, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling HCF-1 / Host Cell Factor C1 with ab289975 at 1/5000 followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human liver. The section was incubated with ab289975 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] - BSA and Azide free (AB290000)

This data was developed using ab289975, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labelling HCF-1 / Host Cell Factor C1 with ab289975 at 1/10000 followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse lung. The section was incubated with ab289975 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] - BSA and Azide free (AB290000)

This data was developed using ab289975, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labelling HCF-1 / Host Cell Factor C1 with ab289975 at 1/500 dilution (0.1μg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor^® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] - BSA and Azide free (AB290000)

This data was developed using ab289975, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 cells labeling HCF-1 / Host Cell Factor C1 with ab289975 at 1/2000 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in NIH/3T3 cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution(Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] - BSA and Azide free (AB290000)

This data was developed using ab289975, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labelling HCF-1 / Host Cell Factor C1 with ab289975 at 1/10000 dilution followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on rat liver. The section was incubated with ab289975 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Lab

Western blot - Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] - BSA and Azide free (AB290000)

This data was developed using ab289975, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : Â 31693902).

Exposure time : 26 seconds.

All lanes:

Western blot - Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] (<a href='/en-us/products/primary-antibodies/hcf-1-host-cell-factor-c1-antibody-epr25290-18-ab289975'>ab289975</a>) at 1/5000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control, whole cell lysate at 10 µg

Lane 2:

HeLa transfected with HCF-1/Host Cell Factor C1 siRNA, whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 208 kDa

Observed band size: 100-150 kDa,300 kDa

false

Lab

Western blot - Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] - BSA and Azide free (AB290000)

This data was developed using ab289975, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 31693902)

Exposure times : Lanes 1-4 : 6 seconds Lanes 5-6 : 26 seconds Lanes 7-8 : 8 seconds

All lanes:

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 20 µg

Lane 3:

NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg

Lane 4:

C2C12 (mouse myoblasts), whole cell lysate at 20 µg

Lane 5:

C6 (rat glial tumor glial cell), whole cell lysate at 20 µg

Lane 6:

PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 20 µg

Lane 7:

Mouse lung tissue lysate at 20 µg

Lane 8:

Mouse testis tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 208 kDa

Observed band size: 100-150 kDa,300 kDa

false

Unconjugated

Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25290-18

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Mouse, Human

Applications

ICC/IF, IHC-P, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Product details

ab290000 is the carrier-free version of ab289975

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Host Cell Factor C1 commonly known as HCF-1 is a large protein with a mass of approximately 203 kDa. It is widely expressed in many tissues and it plays a significant role in cellular processes such as transcription regulation. HCF-1 can be commonly found in the nucleus and cytoplasm and through various alternative names it is sometimes referred to as HCF-1 HCF-1 stores or HCP48BCF. Its mechanical action involves the regulation of transcription through interactions with other transcription factors and cofactors.

Biological function summary

In multiple cellular frameworks HCF-1 operates as a part of the Set1/COMPASS complex which is pivotal for transcriptional coactivation. It engages in the regulation of cell cycle progression acting as a co-regulator with transcription factors associated with cell proliferation. HCF-1 influences chromatin structure permitting changes in gene expression. This dynamic regulation facilitates processes like DNA replication and repair emphasizing its role in maintaining genomic stability.

Pathways

Several cellular pathways involve HCF-1 including the herpes simplex virus (HSV) replication pathway and the E2F transcriptional pathway. In the HSV pathway HCF-1 interacts with viral components to regulate gene expression. In the context of E2F pathways HCF-1 links with E2F transcription factors to control cell cycle entry and progression. Such interactions highlight its central role in coordinating cellular responses to both internal and external stimuli.

HCF-1 connections arise prominently in cancer and neurodevelopmental disorders. Its deregulation is often seen in cancer where it can interact with oncoproteins such as c-Myc modulating oncogenic transcription. HCF-1's role in transcriptional regulation and chromatin remodeling ties it to neurodevelopmental disorders where associated proteins like SETD1A are critical. Understanding these connections highlights the therapeutic potential of targeting HCF-1 in disease contexts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional coregulator (By similarity). Serves as a scaffold protein, bridging interactions between transcription factors, including THAP11 and ZNF143, and transcriptional coregulators (PubMed : 26416877). Involved in control of the cell cycle (PubMed : 10629049, PubMed : 10779346, PubMed : 15190068, PubMed : 16624878, PubMed : 23629655). Also antagonizes transactivation by ZBTB17 and GABP2; represses ZBTB17 activation of the p15(INK4b) promoter and inhibits its ability to recruit p300 (PubMed : 10675337, PubMed : 12244100). Coactivator for EGR2 and GABP2 (PubMed : 12244100, PubMed : 14532282). Tethers the chromatin modifying Set1/Ash2 histone H3 'Lys-4' methyltransferase (H3K4me) and Sin3 histone deacetylase (HDAC) complexes (involved in the activation and repression of transcription, respectively) together (PubMed : 12670868). Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1 (PubMed : 20200153). As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues (PubMed : 20018852). Recruits KMT2E/MLL5 to E2F1 responsive promoters promoting transcriptional activation and thereby facilitates G1 to S phase transition (PubMed : 23629655). Modulates expression of homeobox protein PDX1, perhaps acting in concert with transcription factor E2F1, thereby regulating pancreatic beta-cell growth and glucose-stimulated insulin secretion (By similarity). May negatively modulate transcriptional activity of FOXO3 (By similarity).. (Microbial infection) In case of human herpes simplex virus (HSV) infection, HCFC1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and POU2F1 thereby enabling the transcription of the viral immediate early genes.

See full target information HCFC1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] - BSA and Azide free (AB290000)

Immunocytochemistry/ Immunofluorescence - Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] - BSA and Azide free (AB290000)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] - BSA and Azide free (AB290000)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] - BSA and Azide free (AB290000)

Flow Cytometry (Intracellular) - Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] - BSA and Azide free (AB290000)

Immunocytochemistry/ Immunofluorescence - Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] - BSA and Azide free (AB290000)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] - BSA and Azide free (AB290000)

Western blot - Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] - BSA and Azide free (AB290000)

Western blot - Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] - BSA and Azide free (AB290000)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise