Rabbit Polyclonal HCF-1 / Host Cell Factor C1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human HCFC1 aa 1-300.
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Publications
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- Journal of Cancer 14:1381-13972023Upregulation of HCFC1 expression promoted hepatocellular carcinoma progression through inhibiting cell cycle arrest and correlated with immune infiltration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuaxiang Wang,Meng Yu,Chengkai Yang,Qingsong LiPubMed 37283799
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human HCFC1 aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link P51610
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
| Rat | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Target data
Function
Transcriptional coregulator (By similarity). Involved in control of the cell cycle (PubMed:10629049, PubMed:10779346, PubMed:15190068, PubMed:16624878, PubMed:23629655). Also antagonizes transactivation by ZBTB17 and GABP2; represses ZBTB17 activation of the p15(INK4b) promoter and inhibits its ability to recruit p300 (PubMed:10675337, PubMed:12244100). Coactivator for EGR2 and GABP2 (PubMed:12244100, PubMed:14532282). Tethers the chromatin modifying Set1/Ash2 histone H3 'Lys-4' methyltransferase (H3K4me) and Sin3 histone deacetylase (HDAC) complexes (involved in the activation and repression of transcription, respectively) together (PubMed:12670868). Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1 (PubMed:20200153). As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues (PubMed:20018852). Recruits KMT2E/MLL5 to E2F1 responsive promoters promoting transcriptional activation and thereby facilitates G1 to S phase transition (PubMed:23629655). Modulates expression of homeobox protein PDX1, perhaps acting in concert with transcription factor E2F1, thereby regulating pancreatic beta-cell growth and glucose-stimulated insulin secretion (By similarity). May negatively modulate transcriptional activity of FOXO3 (By similarity). (Microbial infection) In case of human herpes simplex virus (HSV) infection, HCFC1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and POU2F1 thereby enabling the transcription of the viral immediate early genes.
Alternative names
HCF1, HFC1, HCFC1, Host cell factor 1, HCF, HCF-1, C1 factor, CFF, VCAF, VP16 accessory protein
Recommended products
Rabbit Polyclonal HCF-1 / Host Cell Factor C1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human HCFC1 aa 1-300.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human HCFC1 aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link P51610
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Host Cell Factor C1 commonly known as HCF-1 is a large protein with a mass of approximately 203 kDa. It is widely expressed in many tissues and it plays a significant role in cellular processes such as transcription regulation. HCF-1 can be commonly found in the nucleus and cytoplasm and through various alternative names it is sometimes referred to as HCF-1 HCF-1 stores or HCP48BCF. Its mechanical action involves the regulation of transcription through interactions with other transcription factors and cofactors.
- Biological function summary
In multiple cellular frameworks HCF-1 operates as a part of the Set1/COMPASS complex which is pivotal for transcriptional coactivation. It engages in the regulation of cell cycle progression acting as a co-regulator with transcription factors associated with cell proliferation. HCF-1 influences chromatin structure permitting changes in gene expression. This dynamic regulation facilitates processes like DNA replication and repair emphasizing its role in maintaining genomic stability.
- Pathways
Several cellular pathways involve HCF-1 including the herpes simplex virus (HSV) replication pathway and the E2F transcriptional pathway. In the HSV pathway HCF-1 interacts with viral components to regulate gene expression. In the context of E2F pathways HCF-1 links with E2F transcription factors to control cell cycle entry and progression. Such interactions highlight its central role in coordinating cellular responses to both internal and external stimuli.
- Associated diseases and disorders
HCF-1 connections arise prominently in cancer and neurodevelopmental disorders. Its deregulation is often seen in cancer where it can interact with oncoproteins such as c-Myc modulating oncogenic transcription. HCF-1's role in transcriptional regulation and chromatin remodeling ties it to neurodevelopmental disorders where associated proteins like SETD1A are critical. Understanding these connections highlights the therapeutic potential of targeting HCF-1 in disease contexts.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-HCF-1 / Host Cell Factor C1 antibody - N-terminal (ab137618)
5% SDS PAGE
All lanes: Western blot - Anti-HCF-1 / Host Cell Factor C1 antibody - N-terminal (ab137618) at 1/500 dilution
All lanes: HeLa whole cell lysate at 30 µg
Predicted band size: 208 kDa
Immunocytochemistry/ Immunofluorescence - Anti-HCF-1 / Host Cell Factor C1 antibody - N-terminal (ab137618)
ab137618 at 1/200 dilution staining HCF-1 / Host Cell Factor C1 in methanol fixed HeLa cells by Immunofluorescence. Lower image costained with Hoerchst 33342.
Western blot - Anti-HCF-1 / Host Cell Factor C1 antibody - N-terminal (ab137618)
5% SDS PAGE
All lanes: Western blot - Anti-HCF-1 / Host Cell Factor C1 antibody - N-terminal (ab137618) at 1/500 dilution
All lanes: Mouse brain whole cell lysate at 50 µg
Predicted band size: 208 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCF-1 / Host Cell Factor C1 antibody - N-terminal (ab137618)
ab137618 at 1/100 dilution, staining HCF-1 / Host Cell Factor C1 in paraffin embedded BT474 xenograft tissue by Immunohistochemistry.
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