AB54410

Anti-hCG antibody

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(9 Publications)

Rabbit Polyclonal GLHA antibody. Suitable for WB and reacts with Recombinant full length protein - Human samples. Cited in 9 publications.

View Alternative Names

Glycoprotein hormones alpha chain, Anterior pituitary glycoprotein hormones common subunit alpha, Choriogonadotropin alpha chain, Chorionic gonadotrophin subunit alpha, Follicle-stimulating hormone alpha chain, Follitropin alpha chain, Luteinizing hormone alpha chain, Lutropin alpha chain, Thyroid-stimulating hormone alpha chain, Thyrotropin alpha chain, CG-alpha, FSH-alpha, LSH-alpha, TSH-alpha, CGA

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Supplier Data

Western blot - Anti-hCG antibody (AB54410)

This information is only intended as a guide. The optimal dilutions must be determined by the user.

All lanes:

Western blot - Anti-hCG antibody (ab54410)

Lane 1:

Recombinant Human Chorionic Gonadotropin at 0.01 µg

Lane 2:

Recombinant Human Chorionic Gonadotropin at 0.05 µg

Lane 3:

Recombinant Human Chorionic Gonadotropin at 0.25 µg

Lane 4:

Recombinant Human Chorionic Gonadotropin at 1 µg

Predicted band size: 18 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Specificity

This antibody recognises both alpha and beta subunits of hCG.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-4 µg/mL", "WB-species-notes": "<p></p>" } } }

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Product details

This product is manufactured by BioVision, an Abcam company and was previously called 5778 Chorionic Gonadotropin (CG) Antibody. 5778-100 is the same size as the 100 µg size of ab54410.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Human chorionic gonadotropin (hCG) is a glycoprotein hormone composed of two subunits alpha and beta. The beta subunit confers its specific biological activity. Its molecular weight ranges around 36.7 kDa. hCG is produced in the placenta during pregnancy and is also expressed in pituitary cells. Known alternatively as the pregnancy hormone hCG plays a critical role in maintaining the luteal function during the early stages of pregnancy.

Biological function summary

HCG regulates several functions related to reproduction. It supports the corpus luteum in ovaries to secrete progesterone during early pregnancy contributing to the uterine lining's maintenance. Moreover hCG can interact with the luteinizing hormone/chorionic gonadotropin receptor (LHCGR) indicating its involvement in broader reproductive functions. Not as well known hCG may exist in various forms such as hCG intact influencing certain biological pathways differently.

Pathways

Scientists study hCG's role in reproductive signaling and gonadotropin pathways. It interacts closely with luteinizing hormone (LH) with substantial overlap in their pathway functions impacting reproductive health and fertility cycles. hCG's activation of specific receptors like LHCGR plays a role in these pathways connecting it to various fertility-related responses in the human body.

HCG levels can be an indicative marker for certain conditions. Elevated hCG levels may signal trophoblastic diseases such as molar pregnancy or gestational trophoblastic neoplasia. Its abnormal expression might also indicate testicular cancer where it acts as a tumor marker. Both conditions relate to hCG through its shared glycoprotein structure with other hormones like thyroid-stimulating hormone (TSH) which links the hormone to broader endocrine disorder research.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Shared alpha chain of the active heterodimeric glycoprotein hormones thyrotropin/thyroid stimulating hormone/TSH, lutropin/luteinizing hormone/LH, follitropin/follicle stimulating hormone/FSH and choriogonadotropin/CG. These hormones bind specific receptors on target cells that in turn activate downstream signaling pathways.

See full target information CGA

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 13:7387 PubMed37149697

2023

Mitochondrial citrate metabolism and efflux regulate BeWo differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Renee M Mahr,Snehalata Jena,Sereen K Nashif,Alisa B Nelson,Adam J Rauckhorst,Ferrol I Rome,Ryan D Sheldon,Curtis C Hughey,Patrycja Puchalska,Micah D Gearhart,Eric B Taylor,Peter A Crawford,Sarah A Wernimont

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34:9419-9432 PubMed32501590

2020

NPFF increases fusogenic proteins syncytin 1 and syncytin 2 via GCM1 in first trimester primary human cytotrophoblast cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hua Zhu,Bo Peng,Christian Klausen,Yuyin Yi,Yan Li,Siyuan Xiong,Peter von Dadelszen,Peter C K Leung

Analytical and bioanalytical chemistry 411:6837-6845 PubMed31471682

2019

New photothermal immunoassay of human chorionic gonadotropin using Prussian blue nanoparticle-based photothermal conversion.

Applications

Unspecified application

Species

Unspecified reactive species

Guolin Hong,Dongdong Zhang,Yinghao He,Yuanyuan Yang,Ping Chen,Huijing Yang,Zhiyang Zhou,Yinhuan Liu,Yan Wang

Placenta 39:45-54 PubMed26992674

2016

Twist1 is involved in trophoblast syncytialization by regulating GCM1.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyin Lu,Yuxia He,Cheng Zhu,Hongmei Wang,Shiling Chen,Hai-Yan Lin

Reproduction (Cambridge, England) 149:339-46 PubMed25614620

2015

Involvement of nephrin in human placental trophoblast syncytialization.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Li,Ru Zheng,Rui Wang,Xiaoyin Lu,Cheng Zhu,Hai-Yan Lin,Hongmei Wang,Xiaoguang Yu,Jiejun Fu

Placenta 35:907-18 PubMed25175744

2014

The cAMP-responsive element binding protein (CREB) transcription factor regulates furin expression during human trophoblast syncytialization.

Applications

Unspecified application

Species

Unspecified reactive species

Z Zhou,R Wang,X Yang,X-Y Lu,Q Zhang,Y-L Wang,H Wang,C Zhu,H-Y Lin,H Wang

Biology of reproduction 90:117 PubMed24740598

2014

Live cell imaging of in vitro human trophoblast syncytialization.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Wang,Yan-Li Dang,Ru Zheng,Yue Li,Weiwei Li,Xiaoyin Lu,Li-Juan Wang,Cheng Zhu,Hai-Yan Lin,Hongmei Wang

Cell death & disease 4:e593 PubMed23598405

2013

The proprotein convertase furin is required for trophoblast syncytialization.

Applications

Species

Human

Z Zhou,Q Zhang,X Lu,R Wang,H Wang,Y-L Wang,C Zhu,H-Y Lin,H Wang

PloS one 7:e52567 PubMed23285091

2012

Chorionic gonadotropin and its receptor are both expressed in human retina, possible implications in normal and pathological conditions.

Applications

Species

Human

Sladjana Dukic-Stefanovic,Jan Walther,Sebastian Wosch,Gerolf Zimmermann,Peter Wiedemann,Henry Alexander,Thomas Claudepierre

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-hCG antibody

Western blot - Anti-hCG antibody (AB54410)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

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