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AB9582

Anti-hCG beta antibody [5H4-E2]

3

(2 Reviews)

|

(37 Publications)

Anti-hCG beta antibody [5H4-E2] (ab9582) is a mouse monoclonal antibody detecting hCG beta in IHC-P. Suitable for Human.

- Over 20 publications
- Trusted since 2002

View Alternative Names

CGB, CGB5, CGB8, CGB3, Choriogonadotropin subunit beta 3, Choriogonadotropin subunit beta, Chorionic gonadotropin chain beta, CG-beta

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hCG beta antibody [5H4-E2] (AB9582)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hCG beta antibody [5H4-E2] (AB9582)

Formalin-fixed, paraffin-embedded human seminoma tissue stained for hCG beta with ab9582 at a 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hCG beta antibody [5H4-E2] (AB9582)
  • IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hCG beta antibody [5H4-E2] (AB9582)

ab9582 staining hCG beta in human fetal chorionic villus by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Samples were fixed in 4% paraformaldehyde and then incubated with ab9582 in PBT [3% bovine serum albumin (BSA) and 0.5% Triton X-100 in phosphate-buffered saline (PBS)] overnight at 4°C. After extensive washing with PBT, samples were incubated with a secondary antibody conjugated to Alexa-Fluor® 488 for 1 hour at room temperature in the dark. After nuclear staining with Hoechst slides were mounted with PBS/ glycerol.

Image from Spitalieri P et al, Cloning Stem Cells. 2009 Dec;11(4):535-56, Fig 1, DOI: 10.1089/clo.2009.0046.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

5H4-E2

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Native Full Length Protein corresponding to Human CGB3.

P0DN86

Specificity

The antibody recognizes the whole hCG beta and the C-terminal end part of hCG beta, as well as the hCG dimer molecule. It does not react with hCG alpha, FSH, LH, TSH and albumins (evaluated by simple-binding ELISA using 1.00 µg/well purified antigens (WHO standards) and 1.00 µg/ml primary antibody concentrations). This antibody labels the cytoplasm of hCG beta producing (trophoblastic) cells in placenta, mola hydatidosa, choriocarcinoma, embryonal carcinoma and some undifferenciated lung cancers, as well as, the JAR choriocarcinoma cell line. It does not react with cells of normal endometrial, testicular tissues and lung (tested by immunohistochemistry on formalin-fixed, paraffin-embedded tissue sections using the Streptavidin-biotinylated peroxidase method).

Reactivity data

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Product details

What is this antibody validated in?
Anti-hCG beta antibody [5H4-E2] (ab9582) is a mouse monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P) in Human samples.

Trusted by the scientific community
Anti-hCG beta [5H4-E2] (ab9582) was first used in a scientific publication in 2002 and has been cited over 20 times in peer-reviewed journals.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.097% Sodium azide Constituents: Tris HCl, 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HCG beta also known as beta hCG or hCGΒ is a subunit of the human chorionic gonadotropin (hCG) protein. This protein plays critical roles in human reproduction. hCG is a glycoprotein hormone composed of alpha and beta subunits with the beta subunit being unique to hCG and conferring its biological specificity. The mass of the beta hCG is approximately 22.2 kDa. hCG beta is expressed mainly in placental trophoblastic cells during pregnancy where it stimulates the corpus luteum to produce progesterone. This progesterone production is essential to maintain the uterine lining for embryo implantation and growth.
Biological function summary

HCG beta plays a significant role in gestational development. It is not a standalone protein but part of the larger hCG complex conferring specific hormonal activity when paired with the common alpha subunit. This activity involves supporting fetal-placental development and enabling maternal immune tolerance to the embryo. As a marker hCG beta levels are measured in pregnancy tests to confirm conception. In addition to pregnancy-related functions hCG beta has been detected in certain cancers making it useful as a tumor marker. It sometimes gets used in clinical settings to monitor trophoblastic disease and germ cell tumors.

Pathways

HCG beta is intricately involved in reproductive signaling pathways. It plays an important role in the cAMP-PKA pathway by acting through the luteinizing hormone/choriogonadotropin receptor which regulates steroidogenesis in the reproductive system. hCG beta’s activity leads to the stimulation of downstream proteins such as progesterone which modulates various reproductive and systemic effects. The involvement of hCG beta in the Wnt signaling pathway has also been suggested indicating its broader role in cellular growth and differentiation making it an influential factor in both pregnancy and certain oncogenic processes.

HCG beta holds significant importance in oncology and reproductive health. Elevated levels of hCG beta are associated with gestational trophoblastic diseases such as choriocarcinoma a fast-growing cancer originating in the uterus. The protein’s abnormal presence is often an indicator in testicular cancer specifically germ cell tumors where it serves as a marker for diagnosis and treatment monitoring. These connections make hCG beta a valuable target for understanding tumor biology and developing diagnostic strategies. Further interactions with related proteins such as the luteinizing hormone receptor highlight its function in these pathogenic conditions.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Beta subunit of the human chorionic gonadotropin (hCG). hCG is a complex glycoprotein composed of two glycosylated subunits alpha and beta which are non-covalently associated. The alpha subunit is identical to those in the pituitary gonadotropin hormones (LH, FSH and TSH). The beta subunits are distinct in each of the hormones and confer receptor and biological specificity. Has an essential role in pregnancy and maternal adaptation. Stimulates the ovaries to synthesize the steroids that are essential for the maintenance of pregnancy.
See full target information CGB3

Publications (37)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eadr5199 PubMed40815643

2025

Traction force and mechanosensitivity mediate species-specific implantation patterns in human and mouse embryos.

Applications

Unspecified application

Species

Unspecified reactive species

Amélie Luise Godeau,Anna Seriola,Oren Tchaicheeyan,Marc Casals,Denitza Denkova,Ester Aroca,Ot Massafret,Albert Parra,Maria Demestre,Anna Ferrer-Vaquer,Shahar Goren,Anna Veiga,Miquel Solé,Montse Boada,Jordi Comelles,Elena Martínez,Julien Colombelli,Ayelet Lesman,Samuel Ojosnegros

Cells 14: PubMed40643542

2025

Human Extravillous Trophoblasts Require SRC-2 for Sustained Viability, Migration, and Invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Vineet K Maurya,Pooja Popli,Bryan C Nikolai,David M Lonard,Ramakrishna Kommagani,Bert W O'Malley,John P Lydon

eLife 13: PubMed40424181

2025

Comparative analysis of the syncytiotrophoblast in placenta tissue and trophoblast organoids using snRNA sequencing.

Applications

Unspecified application

Species

Unspecified reactive species

Madeline M Keenen,Liheng Yang,Huan Liang,Veronica J Farmer,Rizban E Worota,Rohit Singh,Amy S Gladfelter,Carolyn B Coyne

iScience 28:112099 PubMed40129708

2025

Development of apical out trophoblast stem cell derived organoids to model early human pregnancy.

Applications

Unspecified application

Species

Unspecified reactive species

J Zhou,M A Sheridan,Y Tian,K J Dahlgren,M Messler,T Peng,A Zhao,T Ezashi,L C Schulz,B D Ulery,R M Roberts,D J Schust

Cell 187:6566-6583.e22 PubMed39332412

2024

mTOR activity paces human blastocyst stage developmental progression.

Applications

Unspecified application

Species

Unspecified reactive species

Dhanur P Iyer,Heidar Heidari Khoei,Vera A van der Weijden,Harunobu Kagawa,Saurabh J Pradhan,Maria Novatchkova,Afshan McCarthy,Teresa Rayon,Claire S Simon,Ilona Dunkel,Sissy E Wamaitha,Kay Elder,Phil Snell,Leila Christie,Edda G Schulz,Kathy K Niakan,Nicolas Rivron,Aydan Bulut-Karslioğlu

Cell proliferation 57:e13729 PubMed39161168

2024

The impact of dynamic caudal type homeobox 2 expression on the differentiation of human trophoblast lineage during implantation.

Applications

Unspecified application

Species

Unspecified reactive species

Lujuan Rong,Lifeng Xiang,Zongyong Ai,Baohua Niu,Yaqing Wang,Yu Yin,Chun Feng,Gaohui Shi,Tingwei Chen,Jie Yang,Xi Luo,Yun Bai,Xiaoting Zhou,Xiaoping Liu,Haishan Zheng,Yang Ke,Tianqing Li,Ze Wu

Cell death and differentiation 31:1439-1451 PubMed38965447

2024

TFEB controls syncytiotrophoblast formation and hormone production in placenta.

Applications

Unspecified application

Species

Unspecified reactive species

Marcella Cesana,Gennaro Tufano,Francesco Panariello,Nicolina Zampelli,Chiara Soldati,Margherita Mutarelli,Sandro Montefusco,Giuseppina Grieco,Lucia Vittoria Sepe,Barbara Rossi,Edoardo Nusco,Giada Rossignoli,Giorgia Panebianco,Fabrizio Merciai,Emanuela Salviati,Eduardo Maria Sommella,Pietro Campiglia,Graziano Martello,Davide Cacchiarelli,Diego Luis Medina,Andrea Ballabio

Nature communications 15:5602 PubMed38961108

2024

KAT8-mediated H4K16ac is essential for sustaining trophoblast self-renewal and proliferation via regulating CDX2.

Applications

Unspecified application

Species

Unspecified reactive species

Shilei Bi,Lijun Huang,Yongjie Chen,Zhenhua Hu,Shanze Li,Yifan Wang,Baoying Huang,Lizi Zhang,Yuanyuan Huang,Beibei Dai,Lili Du,Zhaowei Tu,Yijing Wang,Dan Xu,Xiaotong Xu,Wen Sun,Julia Kzhyshkowska,Haibin Wang,Dunjin Chen,Fengchao Wang,Shuang Zhang

Biology of reproduction 110:300-309 PubMed37930227

2023

Shear stress in the intervillous space promotes syncytial formation of iPS cells-derived trophoblasts†.

Applications

Unspecified application

Species

Unspecified reactive species

Asako Inohaya,Yoshitsugu Chigusa,Masahito Takakura,Shingo Io,Min-A Kim,Yu Matsuzaka,Eriko Yasuda,Yusuke Ueda,Yosuke Kawamura,Shiro Takamatsu,Haruta Mogami,Yasuhiro Takashima,Masaki Mandai,Eiji Kondoh

Nature 622:562-573 PubMed37673118

2023

Complete human day 14 post-implantation embryo models from naive ES cells.

Applications

Unspecified application

Species

Unspecified reactive species

Bernardo Oldak,Emilie Wildschutz,Vladyslav Bondarenko,Mehmet-Yunus Comar,Cheng Zhao,Alejandro Aguilera-Castrejon,Shadi Tarazi,Sergey Viukov,Thi Xuan Ai Pham,Shahd Ashouokhi,Dmitry Lokshtanov,Francesco Roncato,Eitan Ariel,Max Rose,Nir Livnat,Tom Shani,Carine Joubran,Roni Cohen,Yoseph Addadi,Muriel Chemla,Merav Kedmi,Hadas Keren-Shaul,Vincent Pasque,Sophie Petropoulos,Fredrik Lanner,Noa Novershtern,Jacob H Hanna
View all publications

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