Anti-hCG beta antibody [5H4-E2]
3
(2 Reviews)
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(37 Publications)
Anti-hCG beta antibody [5H4-E2] (ab9582) is a mouse monoclonal antibody detecting hCG beta in IHC-P. Suitable for Human.
- Over 20 publications
- Trusted since 2002
View Alternative Names
CGB, CGB5, CGB8, CGB3, Choriogonadotropin subunit beta 3, Choriogonadotropin subunit beta, Chorionic gonadotropin chain beta, CG-beta
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hCG beta antibody [5H4-E2] (AB9582)
Formalin-fixed, paraffin-embedded human seminoma tissue stained for hCG beta with ab9582 at a 1/50 dilution in immunohistochemical analysis.
- IHC-P
PubMed
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hCG beta antibody [5H4-E2] (AB9582)
ab9582 staining hCG beta in human fetal chorionic villus by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Samples were fixed in 4% paraformaldehyde and then incubated with ab9582 in PBT [3% bovine serum albumin (BSA) and 0.5% Triton X-100 in phosphate-buffered saline (PBS)] overnight at 4°C. After extensive washing with PBT, samples were incubated with a secondary antibody conjugated to Alexa-Fluor® 488 for 1 hour at room temperature in the dark. After nuclear staining with Hoechst slides were mounted with PBS/ glycerol.
Image from Spitalieri P et al, Cloning Stem Cells. 2009 Dec;11(4):535-56, Fig 1, DOI: 10.1089/clo.2009.0046.
Reactivity data
Product details
Anti-hCG beta antibody [5H4-E2] (ab9582) is a mouse monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P) in Human samples.
Trusted by the scientific community
Anti-hCG beta [5H4-E2] (ab9582) was first used in a scientific publication in 2002 and has been cited over 20 times in peer-reviewed journals.
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Supplementary information
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Biological function summary
HCG beta plays a significant role in gestational development. It is not a standalone protein but part of the larger hCG complex conferring specific hormonal activity when paired with the common alpha subunit. This activity involves supporting fetal-placental development and enabling maternal immune tolerance to the embryo. As a marker hCG beta levels are measured in pregnancy tests to confirm conception. In addition to pregnancy-related functions hCG beta has been detected in certain cancers making it useful as a tumor marker. It sometimes gets used in clinical settings to monitor trophoblastic disease and germ cell tumors.
Pathways
HCG beta is intricately involved in reproductive signaling pathways. It plays an important role in the cAMP-PKA pathway by acting through the luteinizing hormone/choriogonadotropin receptor which regulates steroidogenesis in the reproductive system. hCG beta’s activity leads to the stimulation of downstream proteins such as progesterone which modulates various reproductive and systemic effects. The involvement of hCG beta in the Wnt signaling pathway has also been suggested indicating its broader role in cellular growth and differentiation making it an influential factor in both pregnancy and certain oncogenic processes.
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Target data
Publications (37)
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Science advances 11:eadr5199 PubMed40815643
2025
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Cells 14: PubMed40643542
2025
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eLife 13: PubMed40424181
2025
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iScience 28:112099 PubMed40129708
2025
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Cell 187:6566-6583.e22 PubMed39332412
2024
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Cell proliferation 57:e13729 PubMed39161168
2024
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Cell death and differentiation 31:1439-1451 PubMed38965447
2024
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Nature communications 15:5602 PubMed38961108
2024
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Biology of reproduction 110:300-309 PubMed37930227
2023
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Nature 622:562-573 PubMed37673118
2023
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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