AB204950

Anti-hCG receptor/LHR antibody [8G9A2]

Name: Anti-hCG receptor/LHR antibody [8G9A2] (ab204950) | Abcam
Brand: Abcam Limited
SKU: AB204950
Price: 535 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(4 Publications)

Mouse Monoclonal hCG receptor/LHR antibody. Suitable for Flow Cyt, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Lhcgr aa 1-400.

View Alternative Names

LCGR, LGR2, LHRHR, LHCGR, Lutropin-choriogonadotropic hormone receptor, LH/CG-R, Luteinizing hormone receptor, LHR, LSH-R

2 Images

Flow Cytometry - Anti-hCG receptor/LHR antibody [8G9A2] (AB204950)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-hCG receptor/LHR antibody [8G9A2] (AB204950)

Flow cytometric analysis of Jurkat cells labeling hCG receptor/LHR with ab204950 at 1/200 dilution (green) compared with a negative control (red).

Supplier Data

Western blot - Anti-hCG receptor/LHR antibody [8G9A2] (AB204950)

All lanes:

Western blot - Anti-hCG receptor/LHR antibody [8G9A2] (ab204950) at 1/500 dilution

Lane 1:

HepG2 cell lysate

Lane 2:

Jurkat cell lysate

Lane 3:

SMMC-7721 cell lysate

Predicted band size: 79 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

8G9A2

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

Flow Cyt, WB

applications

Immunogen

Recombinant Fragment Protein within Mouse Lhcgr aa 1-400. The exact immunogen used to generate this antibody is proprietary information.

P30730

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

This product was changed from ascites to supernatant. Lot no's high than GR273983-8 are from Tissue Culture Supernatant.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from tissue culture supernatant.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The hCG receptor also known as LHR LHCGR or LH/CG receptor belongs to the G-protein coupled receptor family and is essential for reproductive processes. It has a mass of around 85 kDa and it is expressed mostly in the ovaries and testes. The receptor binds to the hCG protein also referred to as human chorionic gonadotropin and LH luteinizing hormone. Both the hCG protein and the hCG peptide stimulate the receptor leading to various reproductive functions. The anti-LHR antibodies target this receptor specifically allowing for detailed study and potential clinical applications.

Biological function summary

The hCG receptor plays a significant role in mediating reproductive hormone signaling. As part of a complex with the hCG protein it facilitates the regulation of steroidogenesis and gametogenesis. In females the activation of LHR by the hCG hormone results in the maintenance of the corpus luteum important for progesterone production in early pregnancy. Meanwhile in males LHR influences testosterone production by Leydig cells impacting spermatogenesis significantly.

Pathways

The hCG receptor significantly affects reproductive endocrine pathways that include the cAMP/PKA signaling pathway. Through this pathway the receptor interacts with several related proteins such as adenylate cyclase which further activates cAMP production. The activation of protein kinase A (PKA) facilitates downstream signaling events essential in the ovulation and spermatogenesis processes. The synergy between the hCG receptor and these pathways underlines its biological significance.

The hCG receptor is associated with reproductive health issues like infertility and polycystic ovary syndrome (PCOS). Dysregulation or mutations in LHR can disrupt normal reproductive functions and lead to these disorders. The receptor's interaction with proteins such as FSH receptor (FSHR) further influences the manifestation of these conditions making it a target for therapeutic interventions in reproductive medicine both in clinics and private labs in various places including hCG-focused research in Mexico.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for lutropin-choriogonadotropic hormone (PubMed : 11847099). The activity of this receptor is mediated by G proteins which activate adenylate cyclase (PubMed : 11847099).

See full target information LHCGR

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Tissue engineering and regenerative medicine 18:651-662 PubMed34165777

2021

Rapid Differentiation of Human Embryonic Stem Cells into Testosterone-Producing Leydig Cell-Like Cells In vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Eun-Young Shin,Seah Park,Won Yun Choi,Dong Ryul Lee

Aging 13:1001-1016 PubMed33291079

2020

Conditional deletion of in granulosa cells causes impaired corpora lutea formation and subfertility.

Applications

Unspecified application

Species

Unspecified reactive species

Jinmei Cheng,Yinchuan Li,Yan Zhang,Xiuxia Wang,Fei Sun,Yixun Liu

Oncology letters 16:4271-4278 PubMed30197669

2018

Human chorionic gonadotropin promotes cell proliferation through the activation of c-Met in gastric cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Zhao,Tongtong Zhang,Weidong Xi,Xiaobin Sun,Lingxiao Zhou,Yuanbiao Guo,Cong Zhao,Yu Bao

Cell reports 22:2690-2701 PubMed29514097

2018

Estrogen Deficiency Promotes Hepatic Steatosis via a Glucocorticoid Receptor-Dependent Mechanism in Mice.

Applications

Species

Mouse

Matthew A Quinn,Xiaojiang Xu,Melania Ronfani,John A Cidlowski

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-hCG receptor/LHR antibody [8G9A2]

Flow Cytometry - Anti-hCG receptor/LHR antibody [8G9A2] (AB204950)

Western blot - Anti-hCG receptor/LHR antibody [8G9A2] (AB204950)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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