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AB284722

Anti-Hck antibody [EP2415Y] - BSA and Azide free

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Rabbit Recombinant Monoclonal HCK antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

Tyrosine-protein kinase HCK, Hematopoietic cell kinase, Hemopoietic cell kinase, p59-HCK/p60-HCK, p59Hck, p61Hck, HCK

1 Images
Western blot - Anti-Hck antibody [EP2415Y] - BSA and Azide free (AB284722)
  • WB

Lab

Western blot - Anti-Hck antibody [EP2415Y] - BSA and Azide free (AB284722)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. Exposure time : 1 second (Lane 1); 3 seconds (Lane 2).

All lanes:

Western blot - Anti-Hck antibody [EP2415Y] (<a href='/en-us/products/primary-antibodies/hck-antibody-ep2415y-ab75839'>ab75839</a>) at 1/20000 dilution

Lane 1:

TF-1 (Human bone marrow erythroblast) whole cell lysate at 15 µg

Lane 2:

U-937 (Human histiocytic lymphoma monocyte) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2415Y

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This protein is known to be similar in amino acid sequence to LCK (P06239), FYN (P06241), YES1 (P07947), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request, please contact our Scientific Support for further information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab284722 is the carrier-free version of ab75839.

This product has switched from a hybridoma to recombinant production method on 13th September 2022.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hck also known as Hemopoietic cell kinase is a protein with a mass of approximately 59-61 kDa. Hck belongs to the Src family of tyrosine kinases. It is expressed mainly in cells of the myeloid and B-lymphoid lineages such as macrophages neutrophils and certain B-cells. This protein functions as a non-receptor tyrosine kinase which means it's involved in adding phosphate groups to other proteins to modulate their activity. Such phosphorylation events play significant roles in signal transduction impacting cellular responses.
Biological function summary

Hck is involved in immune system regulation and cell signaling processes. It operates within complexes that facilitate its kinase activity and substrate specificity. Hck influences pathways that regulate immune cell responses like phagocytosis and cell migration. It helps in cellular adhesion and the production of reactive oxygen species both of which are vital for the proper functioning of myeloid cells in the immune response.

Pathways

Several signaling processes use Hck for transmitting signals from the outside of the cell to the inside. Hck participates in the Fc gamma receptor-mediated phagocytosis pathway and the chemokine signaling pathway. In these pathways Hck collaborates with proteins such as Src and Lyn enhancing the immune response and cellular communication. These pathways control important immune functions and cellular responses.

Hck has been implicated in certain cancers including myeloid leukemias where its overactivity can lead to uncontrolled cell proliferation. Additionally Hck plays a role in chronic inflammation seen in disorders like rheumatoid arthritis. In these conditions Hck interacts with other proteins such as Fgr and Yes exacerbating inflammation and disease progression. Understanding Hck's function offers insights into potential therapeutic targets for these health issues.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Non-receptor tyrosine-protein kinase found in hematopoietic cells that transmits signals from cell surface receptors and plays an important role in the regulation of innate immune responses, including neutrophil, monocyte, macrophage and mast cell functions, phagocytosis, cell survival and proliferation, cell adhesion and migration. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as FCGR1A and FCGR2A, but also CSF3R, PLAUR, the receptors for IFNG, IL2, IL6 and IL8, and integrins, such as ITGB1 and ITGB2. During the phagocytic process, mediates mobilization of secretory lysosomes, degranulation, and activation of NADPH oxidase to bring about the respiratory burst. Plays a role in the release of inflammatory molecules. Promotes reorganization of the actin cytoskeleton and actin polymerization, formation of podosomes and cell protrusions. Inhibits TP73-mediated transcription activation and TP73-mediated apoptosis. Phosphorylates CBL in response to activation of immunoglobulin gamma Fc region receptors. Phosphorylates ADAM15, BCR, ELMO1, FCGR2A, GAB1, GAB2, RAPGEF1, STAT5B, TP73, VAV1 and WAS.
See full target information HCK
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