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Rabbit Monoclonal HCK antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human HCK.

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Images

Western blot - Anti-HCK antibody [HL1673] (AB308234), expandable thumbnail
  • Western blot - Anti-HCK antibody [HL1673] (AB308234), expandable thumbnail
  • Immunohistochemistry paraffin embedded sections - Anti-HCK antibody [HL1673] (AB308234), expandable thumbnail
  • Immunohistochemistry paraffin embedded sections - Anti-HCK antibody [HL1673] (AB308234), expandable thumbnail
  • Western blot - Anti-HCK antibody [HL1673] (AB308234), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Synthetic Peptide within Human HCK. The exact immunogen used to generate this antibody is proprietary information. Database link P08631

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested
Mouse
Tested
Tested

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target data

Function

Non-receptor tyrosine-protein kinase found in hematopoietic cells that transmits signals from cell surface receptors and plays an important role in the regulation of innate immune responses, including neutrophil, monocyte, macrophage and mast cell functions, phagocytosis, cell survival and proliferation, cell adhesion and migration. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as FCGR1A and FCGR2A, but also CSF3R, PLAUR, the receptors for IFNG, IL2, IL6 and IL8, and integrins, such as ITGB1 and ITGB2. During the phagocytic process, mediates mobilization of secretory lysosomes, degranulation, and activation of NADPH oxidase to bring about the respiratory burst. Plays a role in the release of inflammatory molecules. Promotes reorganization of the actin cytoskeleton and actin polymerization, formation of podosomes and cell protrusions. Inhibits TP73-mediated transcription activation and TP73-mediated apoptosis. Phosphorylates CBL in response to activation of immunoglobulin gamma Fc region receptors. Phosphorylates ADAM15, BCR, ELMO1, FCGR2A, GAB1, GAB2, RAPGEF1, STAT5B, TP73, VAV1 and WAS.

Alternative names

Tyrosine-protein kinase HCK, Hematopoietic cell kinase, Hemopoietic cell kinase, p59-HCK/p60-HCK, p59Hck, p61Hck, HCK

Recommended products

Rabbit Monoclonal HCK antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human HCK.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Synthetic Peptide within Human HCK. The exact immunogen used to generate this antibody is proprietary information. Database link P08631
Carrier free
Yes
Clone number
HL1673
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-HCK antibody [HL1673] (ab308234), expandable thumbnail

    Western blot - Anti-HCK antibody [HL1673] (ab308234)

    Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HCK antibody [HL1673] (ab308234) diluted at 1/1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

    All lanes: Western blot - Anti-HCK antibody [HL1673] (ab308234) at 1/1000 dilution

    Lane 1: HEK293T cell lysate at 30 µg

    Lane 2: A431 cell lysate at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 59.6 kDa

  • Western blot - Anti-HCK antibody [HL1673] (ab308234), expandable thumbnail

    Western blot - Anti-HCK antibody [HL1673] (ab308234)

    Separated by 10% SDS-PAGE.

    All lanes: Western blot - Anti-HCK antibody [HL1673] (ab308234) at 1/1000 dilution

    All lanes: U937 cell lysate at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 59.6 kDa

  • Immunohistochemistry paraffin embedded sections - Anti-HCK antibody [HL1673] (ab308234), expandable thumbnail

    Immunohistochemistry paraffin embedded sections - Anti-HCK antibody [HL1673] (ab308234)

    Immunohistochemical analysis of paraffin-embedded mouse lung (left) and intestine (right) labeling HCK protein with ab308234 at 1/1000 dilution. Heat mediated antigen retrieval was performed with citrate buffer pH 6.0 for 15 mins.

  • Immunohistochemistry paraffin embedded sections - Anti-HCK antibody [HL1673] (ab308234), expandable thumbnail

    Immunohistochemistry paraffin embedded sections - Anti-HCK antibody [HL1673] (ab308234)

    Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma labeling HCK protein with ab308234 at 1/1000 dilution. Heat mediated antigen retrieval was performed with citrate buffer pH 6.0 for 15 mins.

  • Western blot - Anti-HCK antibody [HL1673] (ab308234), expandable thumbnail

    Western blot - Anti-HCK antibody [HL1673] (ab308234)

    Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HCK antibody [HL1673] (ab308234) diluted at 1/1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

    All lanes: Western blot - Anti-HCK antibody [HL1673] (ab308234) at 1/1000 dilution

    Lane 1: Mouse liver lysate at 50 µg

    Lane 2: Mouse spleen lysate at 50 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 59.6 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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