AB61055

Anti-Hck (phospho Y410) antibody

Name: Anti-Hck (phospho Y410) antibody (ab61055) | Abcam
Brand: Abcam Limited
SKU: AB61055
Rating: 5 (1 reviews)

(1 Review)

(8 Publications)

Rabbit Polyclonal HCK phospho Y410 antibody. Suitable for ELISA, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human Tyrosine-protein kinase HCK phospho Y410 aa 350-450.

View Alternative Names

Tyrosine-protein kinase HCK, Hematopoietic cell kinase, Hemopoietic cell kinase, p59-HCK/p60-HCK, p59Hck, p61Hck, HCK

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-Hck (phospho Y410) antibody (AB61055)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Hck (phospho Y410) antibody (AB61055)

1/500-1/1000 ab61055 staining phosphorylated Hck on HeLa cells in the absence (left) or presence (right) of the immunizing phosphopeptide.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

ICC/IF, ELISA, IHC-P

applications

Immunogen

Synthetic Peptide within Human Tyrosine-protein kinase HCK phospho Y410 aa 350-450. The exact immunogen used to generate this antibody is proprietary information.

P08631

Specificity

Detects target only when phosphorylated at tyrosine 410.

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hck also known as Hemopoietic cell kinase is a protein with a mass of approximately 59-61 kDa. Hck belongs to the Src family of tyrosine kinases. It is expressed mainly in cells of the myeloid and B-lymphoid lineages such as macrophages neutrophils and certain B-cells. This protein functions as a non-receptor tyrosine kinase which means it's involved in adding phosphate groups to other proteins to modulate their activity. Such phosphorylation events play significant roles in signal transduction impacting cellular responses.

Biological function summary

Hck is involved in immune system regulation and cell signaling processes. It operates within complexes that facilitate its kinase activity and substrate specificity. Hck influences pathways that regulate immune cell responses like phagocytosis and cell migration. It helps in cellular adhesion and the production of reactive oxygen species both of which are vital for the proper functioning of myeloid cells in the immune response.

Pathways

Several signaling processes use Hck for transmitting signals from the outside of the cell to the inside. Hck participates in the Fc gamma receptor-mediated phagocytosis pathway and the chemokine signaling pathway. In these pathways Hck collaborates with proteins such as Src and Lyn enhancing the immune response and cellular communication. These pathways control important immune functions and cellular responses.

Hck has been implicated in certain cancers including myeloid leukemias where its overactivity can lead to uncontrolled cell proliferation. Additionally Hck plays a role in chronic inflammation seen in disorders like rheumatoid arthritis. In these conditions Hck interacts with other proteins such as Fgr and Yes exacerbating inflammation and disease progression. Understanding Hck's function offers insights into potential therapeutic targets for these health issues.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Non-receptor tyrosine-protein kinase found in hematopoietic cells that transmits signals from cell surface receptors and plays an important role in the regulation of innate immune responses, including neutrophil, monocyte, macrophage and mast cell functions, phagocytosis, cell survival and proliferation, cell adhesion and migration. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as FCGR1A and FCGR2A, but also CSF3R, PLAUR, the receptors for IFNG, IL2, IL6 and IL8, and integrins, such as ITGB1 and ITGB2. During the phagocytic process, mediates mobilization of secretory lysosomes, degranulation, and activation of NADPH oxidase to bring about the respiratory burst. Plays a role in the release of inflammatory molecules. Promotes reorganization of the actin cytoskeleton and actin polymerization, formation of podosomes and cell protrusions. Inhibits TP73-mediated transcription activation and TP73-mediated apoptosis. Phosphorylates CBL in response to activation of immunoglobulin gamma Fc region receptors. Phosphorylates ADAM15, BCR, ELMO1, FCGR2A, GAB1, GAB2, RAPGEF1, STAT5B, TP73, VAV1 and WAS.

See full target information Tyrosine-protein kinase HCK phospho Y410

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Cells 13: PubMed38920637

2024

CD3ζ-Mediated Signaling Protects Retinal Ganglion Cells in Glutamate Excitotoxicity of the Retina.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Du,Ping Wang,Ning Tian

Molecular cancer therapeutics 23:35-46 PubMed37735104

2023

Bruton's Tyrosine Kinase Inhibitors with Distinct Binding Modes Reveal Differential Functional Impact on B-Cell Receptor Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Li,Renata Sano,Mutiah Apatira,Felix DeAnda,Tarikere Gururaja,Muhua Yang,Greta Lundgaard,Chin Pan,Jing Liu,Yongjiao Zhai,Woo Hyun Yoon,Longcheng Wang,Chris Tse,Andrew J Souers,Chih-Hung Lee

Nature communications 14:4297 PubMed37463911

2023

HCK induces macrophage activation to promote renal inflammation and fibrosis via suppression of autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Man Chen,Madhav C Menon,Wenlin Wang,Jia Fu,Zhengzi Yi,Zeguo Sun,Jessica Liu,Zhengzhe Li,Lingyun Mou,Khadija Banu,Sui-Wan Lee,Ying Dai,Nanditha Anandakrishnan,Evren U Azeloglu,Kyung Lee,Weijia Zhang,Bhaskar Das,John Cijiang He,Chengguo Wei

Bone research 11:17 PubMed36928396

2023

IgSF11-mediated phosphorylation of pyruvate kinase M2 regulates osteoclast differentiation and prevents pathological bone loss.

Applications

Unspecified application

Species

Unspecified reactive species

Hyunsoo Kim,Noriko Takegahara,Yongwon Choi

Cancer immunology, immunotherapy : CII 71:1909-1921 PubMed35039904

2022

Novel inhibitor of hematopoietic cell kinase as a potential therapeutic agent for acute myeloid leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Fernanda Marconi Roversi,Maura Lima Pereira Bueno,Juliete Aparecida Francisco da Silva,Guilherme Rossi Assis-Mendonça,Cristiane Okuda Torello,Rodrigo Nato Shiraishi,Fernando Viera Pericole,Karla Priscila Ferro,Adriana Santos Silva Duarte,Eduardo Magalhães Rego,Sara Teresinha Olalla Saad

British journal of pharmacology 178:4069-4084 PubMed34131920

2021

Bletinib ameliorates neutrophilic inflammation and lung injury by inhibiting Src family kinase phosphorylation and activity.

Applications

Unspecified application

Species

Unspecified reactive species

Ting-I Kao,Po-Jen Chen,Yi-Hsuan Wang,Hsin-Hui Tseng,Shih-Hsin Chang,Tian-Shung Wu,Sien-Hung Yang,Yen-Tung Lee,Tsong-Long Hwang

Frontiers in cell and developmental biology 9:634044 PubMed33842460

2021

Hematopoietic Cell Kinase (HCK) Is a Player of the Crosstalk Between Hematopoietic Cells and Bone Marrow Niche Through CXCL12/CXCR4 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Fernanda Marconi Roversi,Maura Lima Pereira Bueno,Fernando Viera Pericole,Sara Teresinha Olalla Saad

Cancer cell 31:563-575.e5 PubMed28399411

2017

Inhibition of Hematopoietic Cell Kinase Activity Suppresses Myeloid Cell-Mediated Colon Cancer Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Ashleigh R Poh,Christopher G Love,Frederick Masson,Adele Preaudet,Cary Tsui,Lachlan Whitehead,Simon Monard,Yelena Khakham,Lotta Burstroem,Guillaume Lessene,Oliver Sieber,Clifford Lowell,Tracy L Putoczki,Robert J J O'Donoghue,Matthias Ernst

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Hck (phospho Y410) antibody

Immunocytochemistry/ Immunofluorescence - Anti-Hck (phospho Y410) antibody (AB61055)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

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