JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB251388

Anti-HCLS1 antibody [EPR16540] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal HCLS1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.

View Alternative Names

HS1, HCLS1, Hematopoietic lineage cell-specific protein, Hematopoietic cell-specific LYN substrate 1, LckBP1, p75

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCLS1 antibody [EPR16540] - BSA and Azide free (AB251388)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCLS1 antibody [EPR16540] - BSA and Azide free (AB251388)

This data was developed using ab202900, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling HCLS1 with ab202900 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic and weakly nuclear staining on Human tonsil tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCLS1 antibody [EPR16540] - BSA and Azide free (AB251388)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCLS1 antibody [EPR16540] - BSA and Azide free (AB251388)

This data was developed using ab202900, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling HCLS1 with ab202900 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic and nuclear staining on Kupffer cells, while no staining on Human liver tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCLS1 antibody [EPR16540] - BSA and Azide free (AB251388)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCLS1 antibody [EPR16540] - BSA and Azide free (AB251388)

This data was developed using ab202900, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling HCLS1 with ab202900 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. No staining on Human cerebral cortex tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Western blot - Anti-HCLS1 antibody [EPR16540] - BSA and Azide free (AB251388)
  • WB

Supplier Data

Western blot - Anti-HCLS1 antibody [EPR16540] - BSA and Azide free (AB251388)

This data was developed using ab202900, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-HCLS1 antibody [EPR16540] (<a href='/en-us/products/primary-antibodies/hcls1-antibody-epr16540-ab202900'>ab202900</a>) at 1/5000 dilution

Lane 1:

Ramos (Human Burkitt's lymphoma cell line) cell lysate at 20 µg

Lane 2:

Daudi (Human Burkitt's lymphoma cell line) cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 54 kDa

Observed band size: 75 kDa

false

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16540

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
style
left-column

Product details

ab251388 is the carrier-free version of ab202900.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The hematopoietic cell-specific Lyn substrate 1 commonly known as HCLS1 or HS1 is a cytoskeletal protein involved in cell signaling. It has a molecular mass of approximately 75 kDa and is expressed predominantly in hematopoietic cells such as lymphocytes and myeloid cells. HCLS1 functions as an adaptor protein playing an important role in the reorganization of the actin cytoskeleton which is critical for cell motility and morphology.
Biological function summary

The protein is important for signal transduction processes in immune cells particularly during the immune response. HCLS1 becomes phosphorylated upon cell activation allowing it to associate with other signaling proteins. It forms complexes with various partners like Lyn kinase and other SH2-containing proteins facilitating cytoskeletal rearrangement and cellular interactions necessary for effective immune function.

Pathways

HCLS1 is involved in key signaling pathways such as the B-cell receptor (BCR) and T-cell receptor (TCR) signaling pathways. In these pathways it works alongside proteins like SLP-76 and Vav to propagate signals that lead to cellular activation and differentiation. HCLS1's ability to bind and interact with proteins in these pathways highlights its role in orchestrating cellular immune responses.

HCLS1 has been implicated in certain autoimmune conditions and cancers including chronic lymphocytic leukemia (CLL) and systemic lupus erythematosus (SLE). In these contexts aberrant expression or activity of HCLS1 can disrupt normal immune signaling leading to pathological conditions. Additionally its interaction with proteins such as Bcl-2 in CLL suggests a role in apoptosis regulation further linking HCLS1 to disease progression and treatment response.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression.
See full target information HCLS1
style
left-column
style
left-column
style
right-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
  • 104
Primary Antibodies

AB202418

Anti-LRMP antibody [EPR17852]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRMP antibody [EPR17852] (AB202418)

  • 0
  • 0
Primary Antibodies

AB251462

Anti-CXCL7/PBP antibody [EPR19780] - BSA and Azide free

RabMAb|Recombinant

Immunoprecipitation - Anti-CXCL7/PBP antibody [EPR19780] - BSA and Azide free (AB251462)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com