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AB278632

Anti-HCLS1 (phospho Y397) antibody [HS1Y397-F12]

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(1 Publication)

Rabbit Recombinant Monoclonal HCLS1 phospho Y397 antibody. Suitable for Flow Cyt, WB and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human HCLS1 phospho Y397.

View Alternative Names

HS1, HCLS1, Hematopoietic lineage cell-specific protein, Hematopoietic cell-specific LYN substrate 1, LckBP1, p75

4 Images
Flow Cytometry - Anti-HCLS1 (phospho Y397) antibody [HS1Y397-F12] (AB278632)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-HCLS1 (phospho Y397) antibody [HS1Y397-F12] (AB278632)

Flow cytometric analysis of Ramos cells secondary antibody only negative control (blue) or untreated (red) or treated with pervanadate (green) using ab278632 at 0.01µg/mL.

Flow Cytometry - Anti-HCLS1 (phospho Y397) antibody [HS1Y397-F12] (AB278632)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-HCLS1 (phospho Y397) antibody [HS1Y397-F12] (AB278632)

Peptide blocking flow cytometric analysis of Ramos cells secondary antibody only negative control (light blue) or untreated (red) or treated with pervandadate (green) or untreated and blocked with phospho-peptide (black) or treated and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or treated and blocked with non-phospho peptide (purple) using ab278632 at 0.01µg/mL.

Flow Cytometry - Anti-HCLS1 (phospho Y397) antibody [HS1Y397-F12] (AB278632)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-HCLS1 (phospho Y397) antibody [HS1Y397-F12] (AB278632)

Flow cytometric analysis of mouse cells secondary antibody only negative control (blue) or control (red) or stimulated (green) using ab278632 at 0.01µg/mL.

Western blot - Anti-HCLS1 (phospho Y397) antibody [HS1Y397-F12] (AB278632)
  • WB

Supplier Data

Western blot - Anti-HCLS1 (phospho Y397) antibody [HS1Y397-F12] (AB278632)

All lanes:

Western blot - Anti-HCLS1 (phospho Y397) antibody [HS1Y397-F12] (ab278632) at 0.01 µg/mL

Lane 1:

Ramos cell extract, untreated

Lane 2:

Ramos cell extract, treated with 300 nM Thapsigargin for 30 minutes

Predicted band size: 54 kDa

false

  • 578 PE

    PE Anti-HCLS1 (phospho Y397) antibody [HS1Y397-F12]

  • 660 APC

    APC Anti-HCLS1 (phospho Y397) antibody [HS1Y397-F12]

  • 519 FITC

    FITC Anti-HCLS1 (phospho Y397) antibody [HS1Y397-F12]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HS1Y397-F12

Isotype

IgG

Light chain type

kappa

Carrier free

No

Reacts with

Mouse, Human

Applications

Flow Cyt, WB

applications

Immunogen

Synthetic Peptide within Human HCLS1 phospho Y397. The exact immunogen used to generate this antibody is proprietary information.

P14317

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.001-1 µg/mL", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.01 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.001-1 µg/mL", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The hematopoietic cell-specific Lyn substrate 1 commonly known as HCLS1 or HS1 is a cytoskeletal protein involved in cell signaling. It has a molecular mass of approximately 75 kDa and is expressed predominantly in hematopoietic cells such as lymphocytes and myeloid cells. HCLS1 functions as an adaptor protein playing an important role in the reorganization of the actin cytoskeleton which is critical for cell motility and morphology.
Biological function summary

The protein is important for signal transduction processes in immune cells particularly during the immune response. HCLS1 becomes phosphorylated upon cell activation allowing it to associate with other signaling proteins. It forms complexes with various partners like Lyn kinase and other SH2-containing proteins facilitating cytoskeletal rearrangement and cellular interactions necessary for effective immune function.

Pathways

HCLS1 is involved in key signaling pathways such as the B-cell receptor (BCR) and T-cell receptor (TCR) signaling pathways. In these pathways it works alongside proteins like SLP-76 and Vav to propagate signals that lead to cellular activation and differentiation. HCLS1's ability to bind and interact with proteins in these pathways highlights its role in orchestrating cellular immune responses.

HCLS1 has been implicated in certain autoimmune conditions and cancers including chronic lymphocytic leukemia (CLL) and systemic lupus erythematosus (SLE). In these contexts aberrant expression or activity of HCLS1 can disrupt normal immune signaling leading to pathological conditions. Additionally its interaction with proteins such as Bcl-2 in CLL suggests a role in apoptosis regulation further linking HCLS1 to disease progression and treatment response.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression.
See full target information HCLS1 phospho Y397
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:438 PubMed38200007

2024

Disparate macrophage responses are linked to infection outcome of Hantan virus in humans or rodents.

Applications

Unspecified application

Species

Unspecified reactive species

Hongwei Ma,Yongheng Yang,Tiejian Nie,Rong Yan,Yue Si,Jing Wei,Mengyun Li,He Liu,Wei Ye,Hui Zhang,Linfeng Cheng,Liang Zhang,Xin Lv,Limin Luo,Zhikai Xu,Xijing Zhang,Yingfeng Lei,Fanglin Zhang
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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