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Rat Recombinant Monoclonal HCN1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody [7C3] (AB252835), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody [7C3] (AB252835), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody [7C3] (AB252835), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody [7C3] (AB252835), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody [7C3] (AB252835), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Rat
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIHC-P
Human
Tested
Not recommended
Tested
Mouse
Tested
Not recommended
Tested
Rat
Tested
Not recommended
Tested

Tested
Tested

Species
Mouse
Dilution info
0.326 µg/mL
Notes

Human: 99 kDa, Mouse, Rat:110 kDa

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
0.326 µg/mL
Notes

Human: 99 kDa, Mouse, Rat:110 kDa

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
0.326 µg/mL
Notes

Human: 99 kDa, Mouse, Rat:110 kDa

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
0.065 mg/mL
Notes

Use at 1:500 dilution for human and 1:5000 dilution for mouse and rat.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
0.065 mg/mL
Notes

Use at 1:500 dilution for human and 1:5000 dilution for mouse and rat.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
0.065 mg/mL
Notes

Use at 1:500 dilution for human and 1:5000 dilution for mouse and rat.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Hyperpolarization-activated ion channel that are permeable to sodium and potassium ions (PubMed:15351778, PubMed:28086084). Displays lower selectivity for K(+) over Na(+) ions (PubMed:28086084). Contributes to the native pacemaker currents in heart (If) and in the generation of the I(h) current which controls neuron excitability (PubMed:29936235, PubMed:30351409). Participates in cerebellar mechanisms of motor learning (By similarity). May mediate responses to sour stimuli (By similarity).

Alternative names

BCNG1, HCN1, Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1, Brain cyclic nucleotide-gated channel 1, BCNG-1

Recommended products

Rat Recombinant Monoclonal HCN1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
7C3
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The HCN1 protein also known as hyperpolarization-activated cyclic nucleotide-gated channel 1 or HCN1 receptor is an important component in cellular electrical activity. It has a molecular mass of approximately 91 kDa. HCN1 channels are highly expressed in the heart and nervous system particularly in regions like the cortex and hippocampus. Their principal role is serving as pacemaker channels that regulate rhythmic activity in both neurons and cardiac cells.

Biological function summary

HCN1 channels contribute to the generation and modulation of electrical impulses. They belong to a family of ion channels activated by membrane hyperpolarization and modulated by cyclic nucleotides. These channels are part of a larger molecular complex that includes other HCN family members such as HCN2 and HCN4 which together generate coordinated ionic currents essential for maintaining the rhythmic activity.

Pathways

HCN1 channels significantly affect the pacing of cardiac and neuronal circuits. Within the cardiac tissue they partake in regulating heart rate through the If current or pacemaker current together with related proteins like HCN4. In the central nervous system their role extends to neuronal excitability and synaptic transmission interacting with signaling pathways that involve other ion channels and second messengers contributing to complex neuronal network activities.

Associated diseases and disorders

Alterations in HCN1 function or expression levels can lead to various conditions. In the heart dysregulation of HCN1 is associated with arrhythmias potentially involving interactions with proteins like the HCN4 channel. In the nervous system abnormal HCN1 activity has been linked to epilepsy with potential involvement of other ion channel dysfunctions. Understanding these associations provides insight into treatment strategies targeting these specific ion channel pathways.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody [7C3] (ab252835), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody [7C3] (ab252835)

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling HCN1 with ab252835 at 0.065, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Positive staining on rat cerebrum tissue is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody [7C3] (ab252835), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody [7C3] (ab252835)

    Immunohistochemical analysis of paraffin-embedded rat retina tissue labeling HCN1 with ab252835 at 0.065μg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Positive staining on rat retina tissue is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody [7C3] (ab252835), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody [7C3] (ab252835)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling HCN1 with ab252835 at 0.065μg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Positive staining on mouse cerebrum tissue is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody [7C3] (ab252835), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody [7C3] (ab252835)

    Immunohistochemical analysis of paraffin-embedded mouse retina tissue labeling HCN1 with ab252835 at 0.065μg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Positive staining on mouse retina tissue is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody [7C3] (ab252835), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody [7C3] (ab252835)

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling HCN1 with ab252835 at 0.065μg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Positive staining on human esophagus tissue is observed. The section was incubated with ab252835 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Western blot - Anti-HCN1 antibody [7C3] (ab252835), expandable thumbnail

    Western blot - Anti-HCN1 antibody [7C3] (ab252835)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 secs.

    All lanes: Western blot - Anti-HCN1 antibody [7C3] (ab252835) at 0.326 µg/mL

    Lane 1: Mouse brain tissue lysate at 20 µg

    Lane 2: Rat brain tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (Goat Anti-Rat IgG H&L (HRP) ab205720) at 1/10000 dilution

    Predicted band size: 99 kDa

    Exposure time: 3s

  • Western blot - Anti-HCN1 antibody [7C3] (ab252835), expandable thumbnail

    Western blot - Anti-HCN1 antibody [7C3] (ab252835)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 5 secs.

    The negative control and the molecular weight of HCN1 observed is consistent with what has been described in the literature (PMID: 12786975).

    All lanes: Western blot - Anti-HCN1 antibody [7C3] (ab252835) at 0.326 µg/mL

    Lane 1: Human brain tissue lysate at 20 µg

    Lane 2: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (Goat Anti-Rat IgG H&L (HRP) ab205720) at 1/10000 dilution

    Predicted band size: 99 kDa

    Exposure time: 5s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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