AB229340

Anti-HCN1 antibody - C-terminal

Name: Anti-HCN1 antibody - C-terminal (ab229340) | Abcam
Brand: Abcam Limited
SKU: AB229340
Price: 515 USD
Availability: InStock

Be the first to review this product! Submit a review

(6 Publications)

Rabbit Polyclonal HCN1 antibody. C-terminal. Suitable for WB, IHC-P, ICC/IF, IHC-Fr and reacts with Mouse, Rat, Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HCN1.

View Alternative Names

BCNG1, HCN1, Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1, Brain cyclic nucleotide-gated channel 1, BCNG-1

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody - C-terminal (AB229340)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody - C-terminal (AB229340)

Paraffin-embedded mouse brain tissue stained for HCN1 using ab229340 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody - C-terminal (AB229340)

Paraffin-embedded rat brain tissue stained for HCN1 using ab229340 at 1/500 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-HCN1 antibody - C-terminal (AB229340)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HCN1 antibody - C-terminal (AB229340)

4% paraformaldehyde-fixed DIV9 rat E18 primary cortical neurons cells stained for HCN1 (green) using ab229340 at 1/500 dilution in ICC/IF.

Beta Tubulin 3/ Tuj1 (red).

Nuclear counterstain : Fluoroshield with DAPI (blue).

Immunohistochemistry (Frozen sections) - Anti-HCN1 antibody - C-terminal (AB229340)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-HCN1 antibody - C-terminal (AB229340)

Frozen-sectioned adult mouse cerebellum tissue stained for HCN1 (green) using ab229340 at 1/250 dilution in immunohistochemical analysis.

beta Tubulin 3/ TUJ1 (red).

Nuclear counterstain : Fluoroshield with DAPI (blue).

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-HCN1 antibody - C-terminal (AB229340)

Frozen-sectioned adult mouse hippocampus tissue stained for HCN1 (green) using ab229340 at 1/250 dilution in immunohistochemical analysis.

Nuclear counterstain : Fluoroshield with DAPI (blue)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody - C-terminal (AB229340)

Paraffin-embedded mouse retina tissue stained for HCN1 using ab229340 at 1/800 dilution in immunohistochemical abalysis.

Supplier Data

Western blot - Anti-HCN1 antibody - C-terminal (AB229340)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-HCN1 antibody - C-terminal (ab229340) at 1/1000 dilution

All lanes:

SK-N-AS (human neuroblastoma cell line) whole cell extract at 30 µg

Predicted band size: 99 kDa

false

Supplier Data

Western blot - Anti-HCN1 antibody - C-terminal (AB229340)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-HCN1 antibody - C-terminal (ab229340) at 1/1000 dilution

Lane 1:

Mouse brain tissue extract at 50 µg

Lane 2:

Rat brain tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 99 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB, IHC-Fr, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human HCN1. The exact immunogen used to generate this antibody is proprietary information.

O60741

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100 - 1/1000", "IHCFr-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The HCN1 protein also known as hyperpolarization-activated cyclic nucleotide-gated channel 1 or HCN1 receptor is an important component in cellular electrical activity. It has a molecular mass of approximately 91 kDa. HCN1 channels are highly expressed in the heart and nervous system particularly in regions like the cortex and hippocampus. Their principal role is serving as pacemaker channels that regulate rhythmic activity in both neurons and cardiac cells.

Biological function summary

HCN1 channels contribute to the generation and modulation of electrical impulses. They belong to a family of ion channels activated by membrane hyperpolarization and modulated by cyclic nucleotides. These channels are part of a larger molecular complex that includes other HCN family members such as HCN2 and HCN4 which together generate coordinated ionic currents essential for maintaining the rhythmic activity.

Pathways

HCN1 channels significantly affect the pacing of cardiac and neuronal circuits. Within the cardiac tissue they partake in regulating heart rate through the If current or pacemaker current together with related proteins like HCN4. In the central nervous system their role extends to neuronal excitability and synaptic transmission interacting with signaling pathways that involve other ion channels and second messengers contributing to complex neuronal network activities.

Alterations in HCN1 function or expression levels can lead to various conditions. In the heart dysregulation of HCN1 is associated with arrhythmias potentially involving interactions with proteins like the HCN4 channel. In the nervous system abnormal HCN1 activity has been linked to epilepsy with potential involvement of other ion channel dysfunctions. Understanding these associations provides insight into treatment strategies targeting these specific ion channel pathways.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Hyperpolarization-activated ion channel that are permeable to sodium and potassium ions (PubMed : 15351778, PubMed : 28086084). Displays lower selectivity for K(+) over Na(+) ions (PubMed : 28086084). Contributes to the native pacemaker currents in heart (If) and in the generation of the I(h) current which controls neuron excitability (PubMed : 29936235, PubMed : 30351409). Participates in cerebellar mechanisms of motor learning (By similarity). May mediate responses to sour stimuli (By similarity).

See full target information HCN1

style

left-column

Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Gastroenterology research and practice 2025:5790162 PubMed40463822

2025

Changrun Formula Relieves Functional Constipation by Improving Intestinal Motility in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Qiuping Xiao,Yanqiu Hong,Xuesi Geng

Scientific reports 12:7226 PubMed35508511

2022

The transcription factor complex LMO2/TAL1 regulates branching and endothelial cell migration in sprouting angiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshihiro Yamada,Yi Zhong,Shiho Miki,Akiko Taura,Terence H Rabbitts

Frontiers in medicine 9:783931 PubMed35372451

2022

The Effect of SIRT3/Ac-SOD2 Mediated Oxidative Stress and HCN1 Channel Activity on Anesthesia/Surgery Induced Anxiety-Like Behavior in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hui-Hui Miao,Qiang Liu,Ning Wang,Yan-Ping Liu,Chen Chen,Hai-Bi Wang,Hui Huang,Wei-Feng Wu,Jia-Tao Lin,Yong-Kang Qiu,Chuan-Wu Zhang,Cheng-Hua Zhou,Yu-Qing Wu

Frontiers in pharmacology 12:647021 PubMed33995059

2021

The HCN Channel Blocker ZD7288 Induces Emesis in the Least Shrew ().

Applications

Unspecified application

Species

Unspecified reactive species

W Zhong,N A Darmani

American journal of clinical and experimental urol 8:163-176 PubMed33235894

2020

Constitutively active HCN channels constrain detrusor excitability and modulate evoked contractions of human bladder.

Applications

Unspecified application

Species

Unspecified reactive species

Mahendra Kashyap,Nishant Singh,Naoki Yoshimura,Christopher Chermansky,Pradeep Tyagi

Neuropharmacology 162:107805 PubMed31589884

2019

Chronic ethanol exposure alters prelimbic prefrontal cortical Fast-Spiking and Martinotti interneuron function with differential sex specificity in rat brain.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin A Hughes,Elizabeth J Crofton,Todd K O'Buckley,Melissa A Herman,A Leslie Morrow

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HCN1 antibody - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody - C-terminal (AB229340)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody - C-terminal (AB229340)

Immunocytochemistry/ Immunofluorescence - Anti-HCN1 antibody - C-terminal (AB229340)

Immunohistochemistry (Frozen sections) - Anti-HCN1 antibody - C-terminal (AB229340)

Immunohistochemistry (Frozen sections) - Anti-HCN1 antibody - C-terminal (AB229340)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody - C-terminal (AB229340)

Western blot - Anti-HCN1 antibody - C-terminal (AB229340)

Western blot - Anti-HCN1 antibody - C-terminal (AB229340)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise